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Vivaspin protein concentrator spin columns

Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.

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ID	Product Name
28932247	Vivaspin 2 MWCO 10 000
28932258	Vivaspin 2 MWCO 100 000
28932248	Vivaspin 2 MWCO 30 000
28932240	Vivaspin 2 MWCO 3000
28932257	Vivaspin 2 MWCO 50 000
28932245	Vivaspin 2 MWCO 5000
28932360	Vivaspin 20 MWCO 10 000
28932363	Vivaspin 20 MWCO 100 000
28932361	Vivaspin 20 MWCO 30 000
28932358	Vivaspin 20 MWCO 3000
28932362	Vivaspin 20 MWCO 50 000
28932359	Vivaspin 20 MWCO 5000
28932225	Vivaspin 500 MWCO 10 000
28932237	Vivaspin 500 MWCO 100 000
28932235	Vivaspin 500 MWCO 30 000
28932236	Vivaspin 500 MWCO 50 000
28932223	Vivaspin 500 MWCO 5000
28932218	Vivaspin 500 Protein Concentrators, MWCO 3000
28932296	Vivaspin 6 MWCO 10 000
28932319	Vivaspin 6 MWCO 100 000
28932317	Vivaspin 6 MWCO 30 000
28932293	Vivaspin 6 MWCO 3000
28932318	Vivaspin 6 MWCO 50 000
28932294	Vivaspin 6 MWCO 5000

Vivaspin™ ultrafiltration spin columns are designed for fast, non-denaturing concentration and desalting of proteins and other biological samples.

Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
Sample protection: Patented Vivaspin dead-stop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
Ease-of-use: One-step sample concentration in a single tube, for minimal sample handling and reduced sample loss.
High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 μL to 20 mL, with a range of molecular weight cut-off (MWCO) values from Mr 3000 to 100 000.
Download the Vivaspin protein concentrator data file to learn more and to determine the right MWCO (molecular weight cut-off) and volume for your enrichment study.

Spin column protein concentration – how does it work?

Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for one-step biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.

How to select the right spin column for your application

Sample volume and molecular weight of the target biomolecule(s) are the primary factors to consider when choosing a spin column. Vivaspin offers a choice of membranes to cover different ultrafiltration requirements (Table 1).

Tip: For maximum recovery, select a MWCO value at least 50% smaller than the molecular size of the species of interest.

Table 1. Select the appropriate Vivaspin product from the intersection of the molecular weight cutoff value (MWCO value) and the volume range for your sample

		MWCO value
Volume range	Product	3 000	5 000	10 000	30 000	50 000	100 000
strong>100-500 μl	Vivaspin 500	28-9322-18	28-9322-23	28-9322-25	28-9322-35	28-9322-36	28-9322-37
strong>400 μl to 2 ml	Vivaspin 2	28-9322-40	28-9322-45	28-9322-47	28-9322-48	28-9322-57	28-9322-58
strong>2-6 ml	Vivaspin 6	28-9322-93	28-9322-94	28-9322-96	28-9322-17	28-9322-18	28-9322-19
strong>5-20 ml	Vivaspin 20	28-9322-58	28-9322-59	28-9322-60	28-9322-61	28-9322-62	28-9322-63

To learn more, download the Vivaspin protein concentrator data file.

Still not sure what MWCO and volume are right for you? Contact one of our experts today.

Applications for ultrafiltration spin columns

Vivaspin ultrafiltration spin columns are a rapid and flexible solution for protein concentration and small-scale preparation of biological molecules directly from cell lysates and biological fluids, following upstream purification steps. They can also be used prior to downstream analyses, such as gel electrophoresis, liquid chromatography (LC), mass spectrometry (MS), and LC-MS. Examples include:

Concentration of urine prior to electrophoresis for identification and diagnosis of multiple myeloma and related serum protein disorders.
Concentration of recombinant human IFN-g following purification from culture supernatant.

Effective enrichment of low-M(r) components from human plasma for proteomic analysis to identify plasma-based biomarkers in disease

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Product	Product Name	Molecular Weight [MW] Cut-Off All 5000 10000 100000 30000 50000 3000	Volume All 2-6 ml 100-500 µl 400 µl - 2 ml 5-20 ml	Price
28932218	Vivaspin 500 Protein Concentrators, MWCO 3000	3000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932223	Vivaspin 500 MWCO 5000	5000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932225	Vivaspin 500 MWCO 10 000	10000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932235	Vivaspin 500 MWCO 30 000	30000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932236	Vivaspin 500 MWCO 50 000	50000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932237	Vivaspin 500 MWCO 100 000	100000	100-500 µl	111.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932240	Vivaspin 2 MWCO 3000	3000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932245	Vivaspin 2 MWCO 5000	5000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932247	Vivaspin 2 MWCO 10 000	10000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932248	Vivaspin 2 MWCO 30 000	30000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932257	Vivaspin 2 MWCO 50 000	50000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932258	Vivaspin 2 MWCO 100 000	100000	400 µl - 2 ml	138.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932293	Vivaspin 6 MWCO 3000	3000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932294	Vivaspin 6 MWCO 5000	5000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932296	Vivaspin 6 MWCO 10 000	10000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932317	Vivaspin 6 MWCO 30 000	30000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932318	Vivaspin 6 MWCO 50 000	50000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932319	Vivaspin 6 MWCO 100 000	100000	2-6 ml	153.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932358	Vivaspin 20 MWCO 3000	3000	5-20 ml	144.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932359	Vivaspin 20 MWCO 5000	5000	5-20 ml	143.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932360	Vivaspin 20 MWCO 10 000	10000	5-20 ml	143.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932361	Vivaspin 20 MWCO 30 000	30000	5-20 ml	143.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932362	Vivaspin 20 MWCO 50 000	50000	5-20 ml	143.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
28932363	Vivaspin 20 MWCO 100 000	100000	5-20 ml	143.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock

Vivaspin protein concentrator spin columns

Vivaspin protein concentrator spin columns

Spin column protein concentration – how does it work?

How to select the right spin column for your application

Applications for ultrafiltration spin columns

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Vivaspin protein concentrator spin columns

Vivaspin protein concentrator spin columns

Spin column protein concentration – how does it work?

How to select the right spin column for your application

Applications for ultrafiltration spin columns

Select your model

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