Sensor Chip SA

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This high-affinity streptavidin (SA) sensor chip immobilizes biotinylated molecules for surface plasmon resonance (SPR) interaction analysis on Biacore systems.

Versatile: immobilizes biotinylated peptides, proteins, nucleic acids, or carbohydrates
Ready-to-use: streptavidin is pre-immobilized, ready for fast, high-affinity ligand capture
Robust: streptavidin-biotin is one of the strongest non-covalent interactions in nature
Non-covalent: a convenient alternative for ligands refractory to covalent immobilization
Standardized ligand orientation: controlled biotinylation enables oriented capture

Streptavidin-biotin capture

Sensor Chip SA provides a surface of streptavidin covalently attached to a carboxymethyldextran matrix. Dissociation of biotinylated ligands from the surface of the chip is generally negligible during the course of a Biacore analysis because the affinity of streptavidin for biotin is extremely high, with an equilibrium dissociation constant of about 10-15 M. High binding capacity, reproducibility, and chemical resistance give excellent performance over a broad range of applications. The surface of Sensor Chip SA is resistant to 1-minute pulses of many agents commonly used in SPR sensor chip washing and regeneration protocols (Table 1).

Agent	Concentration
Acetonitrile	30%
DMSO	10%
DTE	0.1 M
EDTA	0.35 M
Ethanol	70%
Ethanolamine	1 M
Ethynel glycol	100%
Formamide	40%
Formic acid	20%
Glycine pH 1.5 to 3.0	100 mM
HCI	100 mM
Imidazole	300 mM
MgCI2	4 M
NaOH	100 mM
NaCI	5 M
SDS	0.5%
Surfactant P20	5%
Urea	8 M

Table 1. Common agents compatible with Sensor Chip SA (see also Instruction 22-0564-88 AD)

Biacore Sensor Chip SA applications

Biotin has become a popular molecular tag for capture and interaction studies because it is stable and rarely interferes with target activity or structure due to its small size. Biotin can be conveniently conjugated to a wide range of target molecules by chemical or enzymatic means. Applications for Sensor Chip SA are therefore broad. Examples include:

Sensor chip formats for Biacore systems

Biacore sensor chips are available in two formats:

“S series” sensor chips are for use with Biacore 8K+, Biacore 8K, Biacore S200, Biacore T200, and Biacore 4000 SPR systems.
Sensor chips not designated with “S series” are designed for Biacore X100, Biacore 3000 and Biacore C SPR systems.

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Product	Product Name	Includes All 1 sensor chip 3 sensor chips	Price
29104992	Series S Sensor Chip SA, pack of 1	1 sensor chip	464.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
BR100531	Series S Sensor Chip SA, pack of 3	3 sensor chips	924.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
BR100398	Sensor Chip SA, pack of 1	1 sensor chip	261.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock
BR100032	Sensor Chip SA, pack of 3	3 sensor chips	698.00 USD	Add to cart Get Quote In Stock Stock on request Limited stock

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Sensor Chip SA

Sensor Chip SA

Streptavidin-biotin capture

Biacore Sensor Chip SA applications

Sensor chip formats for Biacore systems

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