High-quality nylon membranes are suitable for filtering aqueous solutions and most organic solvents. The membranes are suitable for use with a wide range of biological preparations and can be used where other membranes are unsuitable or difficult to use. Nylon membranes are hydrophilic, removing the need for wetting agents that could be extracted when filtering aqueous solutions. The membranes are flexible, durable and tear resistant, and can be autoclaved at 135°C. Applications include: filtration of aqueous and organic mobile phases, vacuum degassing, filtration of tissue culture media, microbiological media, buffers, and solutions.