DeltaVision localization microscopy (DLM) is a super-resolution microscopy option for when your biology presents the most challenging of resolution questions. Localization microscopy has its foundations in single molecule imaging techniques which determine the position of fluorophores by statistically localizing their position within an image.

Only one (or a few) fluorophores are allowed to fluoresce at a given time. Control of the fluorescence is achieved using photoactivatable proteins, photoswitchable dyes, or ground state depletion systems combined with appropriate sample buffer solutions. DLM uses the proprietary Dense Stochastic Sampling Imaging (DSSI) algorithm that can determine the location of the fluorophores in overlapping diffraction limited spots. This differs from other localization microscopy techniques which rely on single Gaussian fitting models, which would throw away all events that have overlapping events.

Benefits

  • DLM enables processing of higher density image data making it possible to use denser sample labeling.
  • Provides localization precision down to 20 nm laterally (requires appropriate labeling density and signal-to-noise ratio).
  • Supports all standard approaches to sample labeling for single molecule localization microscopy; photoconversion (dSTORM), photoactivation, and photoswitchable fluorophore biological preparations.


Viral clusters captured with single molecule localization microscopy

Viral clusters on a cell membrane (Kielian Lab, Albert Einstein). DLM shows viral clusters that are 60-80 nm

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