Unlike confocal microscopy approaches, widefield microscopy illuminates the entire sample at once. The images captured contain both in-focus and out-of-focus light. This out-of-focus light makes raw data appear blurry and can mask small or dim structures. Widefield data can be significantly improved with deconvolution. Deconvolution utilizes a measured point spread function (PSF)—a model of how light spreads through microscope optics—to reassign blurred light back to its original point source. Small, fine, or dim structures that may have been masked by out-of-focus blur in raw data are suddenly detectable, and data at lower signal-to-background levels can be collected, preserving cell health.

The thoughtful widefield imaging mode designed and optimized algorithm ensures that users can focus on their biology and trust the microscope to do the rest.

Benefits

  • Utilizing deconvolution with widefield imaging produces higher quality images due to significantly improved contrast.
  • Configurable up to four cameras, which enables true simultaneous imaging in four wavelengths.
  • Burst mode frame rates for up to four wavelengths simultaneously of:
    • > 375 fps at 512 × 512 (> 1500 images/s)
    • > 735 fps at 256 × 256 (> 2940 images/s)
  • Proprietary deconvolution algorithm is linear and quantitative (no intensity values are added or subtracted) making data quantitation possible.
  • Easily add a photokinetic event within any widefield experiments with just milliseconds between PK event and image acquisition. (requires TIRF or confocal module in configuration).

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