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Dipstick colorimetric assays

Assay development with dry chemistry, providing a colorimetric change that indicates a result

Critical components for dipstick colorimetric assays

Critical components for dipstick colorimetric assays

Dipstick colorimetric assays, also referred to as dry chemistry assays, are a diagnostic test in which a cellulose pad is impregnated with a color reagent, and are widely used in everything from urine testing to environmental assays. The base cellulose is a key part of the system, and the correct choice of absorbency, wicking rate, and wet strength are critical to producing a working assay.

Knowledge Article
Selection guide – cellulose materials for dipstick colorimetric assays
January 01, 2024

Our range of cellulose materials offer highly consistent and inert substrates for absorption of the active chemicals required for development of dipstick tests. The purity of the cellulose base material coupled with our quality manufacturing practices make these papers an excellent choice for large-scale manufacturing.

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Guide
Checklist: 10 essentials for choosing a collaborator for diagnostic assay development
January 01, 2024

There is more pressure than ever to quickly commercialize diagnostic assays, as molecular and immunodiagnostics tests using nucleic acids and proteins are now an essential component in the early detection and evaluation of many diseases.

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Urine analysis using dipsticks

Advantages of dipstick assays

  • Basic, single layer, dipstick assays are simple to use and require no sample prep or optical readout.
  • Total assay response time is within minutes
  • Very sensitive assays with as little as a few hundred microliters of sample liquid
  • Easy to multiplex and commonly done in medical diagnostics

Challenges with dipstick assays

Challenges with dipstick assays

  • Complex, multilayer or multiplexed dipstick assays require skilled users to administer the test and read results
  • Requires a liquid sample such as urine, blood or water and is unlikely to convert solid samples to liquid
  • Developmentally complex – multistep reactions require multiple pads that cascade into each other
  • Stacked pad assays are tricky to read as the results show on the bottom of the pad which is adhered to plastic dipstick.