Next generation sequencing (NGS)
Next Generation Sequencing (NGS) is a massively parallel or deep sequencing method which allows for simultanous reading of the sequence of nucleotides in a section of a genome or the entire genome.
An NGS library is the collection of DNA fragments for sequencing. The NGS libraries requires the ligation of specific adapter oligonucleotide to the ends of the fragmented DNA to create a library which is then sequenced.
NGS data analysis can be done by using bioinformatics tools to align the sample sequence (which is output by the sequencer) to a reference sequence from a database. Depending on the analysis method, confidence intervals and alignment score settings, the bioinformatics software determines how similar the sample sequence is to the reference sequence.