Troubleshooting purity issues – top tips

Troubleshooting purity issues – top tips

After protein purification you expect highly pure protein. If your purified protein isn’t making the grade, these troubleshooting tips may help.
How to fix asymmetrical peaks

How to fix asymmetrical peaks

Chromatography books say that chromatogram peaks should be symmetrical. But what about real life in the lab? Many things cause “fronting” or “tailing” peaks. Good news – most are easy to fix.
High pressure stressing you out?

High pressure stressing you out?

Are your chromatography system’s pressure alarms and slow flow rates giving you headaches? Then you’ll want to read this article.
Quick fixes for retention time issues

Quick fixes for retention time issues

Is your protein eluting at the wrong time? Does it elute too early, too late, or maybe not at all? Learn how to quickly identify possible causes of unexpected retention times and how to fix them.

Troubleshooting protein recovery issues

Troubleshooting protein recovery issues

Are you frustrated that you aren’t getting the amount of pure protein that you expected? We’ve got some tips for that.
Inspect your column to ensure you get it right

Inspect your column to ensure you get it right

Have you ever seen bubbles in the resin bed of your chromatography column? Or space between the resin bed and the adaptor? Here are some things to check before your run.