Troubleshooting purity issues – top tips
After protein purification you expect highly pure protein. If your purified protein isn’t making the grade, these troubleshooting tips may help.
How to fix asymmetrical peaks
Chromatography books say that chromatogram peaks should be symmetrical. But what about real life in the lab? Many things cause “fronting” or “tailing” peaks. Good news – most are easy to fix.
Quick fixes for retention time issues
Is your protein eluting at the wrong time? Does it elute too early, too late, or maybe not at all? Learn how to quickly identify possible causes of unexpected retention times and how to fix them.
Troubleshooting protein recovery issues
Are you frustrated that you aren’t getting the amount of pure protein that you expected? We’ve got some tips for that.