Protein purification principles

Affinity chromatography – principles

This is a powerful technique that separates proteins based on the reversible interaction between the target protein and binding substance (ligand).

Hydrophobic interaction chromatography principles

It separates biomolecules according to differences in their hydrophobicity.

Removing affinity tags from tagged proteins

Ion exchange chromatography – principles

Size exclusion chromatography – principles

Size exclusion chromatography, or gel filtration, is the simplest and mildest liquid chromatography technique.

Rapid size exclusion chromatography

Quickly analyze purified antibodies or other proteins using our prepacked Superdex and Superdex Increase columns.

Principles of Multimodal Chromatography (MMC)

Protein purification systems for research

Using ÄKTA pure – a scientist’s perspective

In this video Dr Fabri summarizes his conclusions from using ÄKTA pure to automate a two-step protein purification process.

ÄKTA avant – overview

ÄKTA start protein purification system – overview

ÄKTA start is a compact, easy-to-use system that provides an affordable alternative to manual protein purification.

ÄKTA avant – system features

ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes.

Protein purification how-to videos

ÄKTA tutorial series: How to prevent columns from drying out using a syringe

ÄKTA tutorial series: How to determine column specific pressure limits for SEC columns

ÄKTA system tutorials: Priming system and sample pumps on ÄKTAexplorer, ÄKTApurifier, and ÄKTA FPLC

ÄKTA system tutorials: Calibrating the pressure offset on ÄKTA avant and ÄKTA pure

ÄKTA system tutorials: Calibrating the pressure offset on ÄKTA explorer, ÄKTA purifier and ÄKTA FPLC

ÄKTA system tutorials: Priming system and sample pumps on ÄKTA avant and ÄKTA pure

ÄKTA system tutorials: Replacing the online filter on ÄKTAexplorer, ÄKTApurifier and ÄKTA FPLC

How to pack a HiScale column

ÄKTA system tutorial: Avoid getting air into the column

Step-by-step instructions on connecting a size exclusion chromatography column drop-to-drop to avoid getting air into your column.

ÄKTA system tutorial: Determine column efficiency

Run and evaluate column performance test to determine efficiency of your packed column.

How to prevent column from drying out using an ÄKTA system

HiTrap Protein A & Protein G column - Tips & Tricks

Isolate IgG and IgG subclasses from cell culture supernatants and serum with HiTrap Protein G HP and HiTrap Protein A HP columns.

HisTrap FF crude columns - Tips & Tricks

HisTrap FF crude columns allow you to purify histidine-tagged (his-tagged) proteins directly from unclarified lysates.

Rapid gel filtration - Tips & Tricks

UNICORN 7 tutorial – peak area calculations

Learn how to adjust the baseline range and peak limits using UNICORN software evaluation module.