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This is a powerful technique that separates proteins based on the reversible interaction between the target protein and binding substance (ligand).
It separates biomolecules according to differences in their hydrophobicity.
Size exclusion chromatography, or gel filtration, is the simplest and mildest liquid chromatography technique.
Quickly analyze purified antibodies or other proteins using our prepacked Superdex and Superdex Increase columns.
In this video Dr Fabri summarizes his conclusions from using ÄKTA pure to automate a two-step protein purification process.
ÄKTA start is a compact, easy-to-use system that provides an affordable alternative to manual protein purification.
ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes.
Step-by-step instructions on connecting a size exclusion chromatography column drop-to-drop to avoid getting air into your column.
Run and evaluate column performance test to determine efficiency of your packed column.
Isolate IgG and IgG subclasses from cell culture supernatants and serum with HiTrap Protein G HP and HiTrap Protein A HP columns.
HisTrap FF crude columns allow you to purify histidine-tagged (his-tagged) proteins directly from unclarified lysates.
Learn how to adjust the baseline range and peak limits using UNICORN software evaluation module.