Protein transfer

Electrotransfer is the most common technique for moving proteins from gels to membranes. Wet and semidry transfer methods take advantage of the same electromobility principles as gel electrophoresis. Find out how these two common methods compare and in which situations each is most appropriate.

Assessing the efficiency of protein transfer can reveal valuable information for the success of an experiment involving Western blotting. Total protein stains provide straightforward methods for checking transfer. Find out what stains can be used at this step and how they differ.