FAQ

Accessories

1. Assemble the Normal flow filter (NFF) inlet to the flow kit.
2. Evacuate air by filling NFF with liquid. Use the system to pump liquid  through the system to the NFF.
3. Assemble the NFF to the column inlet. Extra tubing may be needed to prolong the flow kit. Please make sure the tubing is not folded and does not contain any air.

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# Product Name Product Code Price
TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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Item Comment
1 Flow kit for flow rates 7.5 l/h-510 l/h, High Flow Kit 28-9301-83.
2 TC Clamp 25 mm, 28-9381-90 (Stainless steel). 5 clamps are supplied with the column.
3

TC Gasket 25 mm i.d. 15.8 mm, 28-9381-88. 5 gaskets supplied with the column. 

TC Gasket 25 mm i.d. 15 mm, 44-5492-88. 2 gaskets supplied with the flow kit.

4 For appropriate tubing i.d. refer to table below.
5 TC Gasket Kit 25 mm, i.d. 9.4 mm, 28-9381-87.

To isolate the column from the system use Manual Diaphragm Valve, 25 mm TC, i.d. 15,75 mm 28-9382-07 or alternatively only Blind flange 25 mm incl. gasket 18-1001-25 and a TC Clamp 25 mm. Please verify that the pack sizes of clamps and gaskets correspond to your needs.

 

Column tube and flow kit connections

Column Column connection tube material Column tube i.d. Flow kit i.d. TC connection
AxiChrom 300 Stainless steel ASTM 316L 9.4 mm 9.4 mm TC 25 mm
AxiChrom 300 Polypropylene (PP) 10 mm 9.4 mm TC 25 mm

 

Tubing

Tubing i.d. Code No
Tube S-Cor 1/2" (i.d.=12.7), TC25 i.d.= 9.4, 2000 mm 28-9381-25
Tube S-Cor 1/2" (i.d.=12.7), TC25 i.d.= 9.4, 5000 mm 28-9381-26

 

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# Product Name Product Code Price
2 Clamp, 25 mm TC 28938190 132.59 USD Add to cart
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3 Gasket, 25 mm TC, i.d. 15.8 mm 28938188 267.00 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 Tubing, 2 m, i.d. 12.7 mm, 25 mm TC 28938125 2 646.00 USD Add to cart
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4 Tubing, 5 m, i.d. 12.7 mm, 25 mm TC 28938126 4 129.00 USD Add to cart
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5 Gasket, 25 mm TC, i.d. 12.5 mm 28938187 238.00 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Manual Diaphragm Valve, 25 mm TC, i.d.15,75 mm 28938207 1 375.00 USD Add to cart
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29032784 Gradient upgrade ÄKTA ready
If you already own an ÄKTA ready system, this kit will allow you to upgrade your ÄKTA ready to enable gradient elution if the system serial number is 1762375 or higher. Please contact your local Cytiva representative to discuss the possibility of upgrading your ÄKTA ready system.

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Item Comment
1

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

2 For appropriate tubing i.d. refer to table below.
3 TC Gasket 25 mm i.d. 15 mm 44-5492-88, gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect the column to the flow kit.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene). Four clamps needed to connect to system.
5

TC Gasket Kit 25 mm, i.d. 6.5 mm, 28-9667-37. Two gaskets i.d. 6.5 mm needed to connect the column to the system.

6 TC End Cap, 25 mm TC, 28-9667-91.
7 Connector, 25 mm TC - UNF 5/16" Male, short, 18-1170-08.
8 O-Ring, 3 × 1 mm 18-1169-12.

To isolate the column from the system use 2-port, 2-way Valve i.d. 6 mm, 44-5501-00 (left) or 44-5501-01 (right) or alternatively only TC End Cap, 25 mm TC, 28-9667-91, TC Clamp 25 mm and a gasket. Please verify that the pack sizes of clamps and gaskets correspond to your needs.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 140 3.2 mm1 or 4.8 mm2 25 mm TC
AxiChrom 200 6.4 mm 25 mm TC

1 It is recommended to use tubing with id 3.2 mm for media with an approximate bead size < 50 µm.

2 For media with an approximate bead size > 50 µm it is recommended to use tubing with id 4.8 mm.

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# Product Name Product Code Price
3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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6 TC end cap 28966791 324.00 USD Add to cart
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7 Connector, 25 mm TC - UNF 5/16" Male, short 18117008 169.94 USD Add to cart
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8 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
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2 port, 2 way valves i.d. 6 mm (left) 44550100 418.00 USD Add to cart
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2 port, 2 way valves i.d. 6 mm (right) 44550101 409.00 USD Add to cart
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Item Comment
1 Flow kit for flow rates 7.5 l/h-510 l/h, High Flow Kit 28-9301-83.
2 TC Clamp 25 mm, 28-9381-90 (Stainless steel). 5 clamps are supplied with the column.
3

TC Gasket 25 mm i.d. 15.8 mm, 28-9381-88. 5 gaskets supplied with the column. 

TC Gasket 25 mm i.d. 15 mm, 44-5492-88. 2 gaskets are supplied with the flow kit.

4 For appropriate tubing i.d., refer to the tables below.
5 TC Gasket Kit 25 mm, i.d. 9.4 mm, 28-9381-87.
6 Reducer i.d. 9.4 mm - i.d. 15.75 mm 28-9381-74. 2 reducers are needed to connect column to system.

To isolate the column from the system use Manual Diaphragm Valve, 25 mm TC, i.d. 15,75 mm 28-9382-07 or alternatively only Blind flange 25 mm incl. gasket 18-1001-25 and a TC Clamp 25 mm. Please verify that the pack sizes of clamps and gaskets correspond to your needs.

 

Column tube and flow kit connections

Column Column connection tube material Column tube i.d. Flow kit i.d. TC connection
AxiChrom 400 Stainless steel ASTM 316L 15.75 mm 9.4 mm TC 25 mm
AxiChrom 400 Polypropylene (PP) 14.2 mm 9.4 mm TC 25 mm
AxiChrom 450 Stainless steel ASTM 316L 15.75 mm 9.4 mm TC 25 mm
AxiChrom 450 Polypropylene (PP) 14.2 mm 9.4 mm TC 25 mm

 

Tubing

Tubing Code No
Tube S-Cor 1/2" (i.d.=12,7), TC25 i.d.=9.4, 2000 mm 28-9381-25
Tube S-Cor 1/2" (i.d.=12,7), TC25 i.d.=9.4, 5000 mm 28-9381-26
Tube U-Cor 3/4" (i.d.=19,1), TC25 i.d.=15.75, 2000 mm 28-9381-27
Tube U-Cor 3/4" (i.d.=19,1), TC25 i.d.=15.75, 2000 mm 28-9381-28

 

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# Product Name Product Code Price
2 Clamp, 25 mm TC 28938190 132.59 USD Add to cart
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3 Gasket, 25 mm TC, i.d. 15.8 mm 28938188 267.00 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 Tubing, 2 m, i.d. 12.7 mm, 25 mm TC 28938125 2 646.00 USD Add to cart
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4 Tubing, 5 m, i.d. 12.7 mm, 25 mm TC 28938126 4 129.00 USD Add to cart
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4 Tubing, 2 m, i.d. 19.1 mm, 25 mm TC 28938127 2 850.00 USD Add to cart
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4 Tubing, 5 m, i.d. 19.1 mm, 25 mm TC 28938129 5 671.00 USD Add to cart
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5 Gasket, 25 mm TC, i.d. 12.5 mm 28938187 238.00 USD Add to cart
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6 Concentric Reducer, 25 mm TC (i.d. 9.4 mm) to 25 mm TC (i.d. 15.75 mm) 28938174 587.00 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Manual Diaphragm Valve, 25 mm TC, i.d.15,75 mm 28938207 1 375.00 USD Add to cart
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# Product Name Product Code Price
TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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Termination plug 18111859 22.68 USD Add to cart
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Mains cable 220 V 19244801 89.86 USD Add to cart
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Communications cable RJ45-RJ45/IP67, length = 10 m 28404163 212.00 USD Add to cart
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Pneu. air tube o.d. 10 mm, i.d. 8 mm, L=10 m 28407194 173.34 USD Add to cart
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Wheel without brake 28931366 162.20 USD Add to cart
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Castor with brake 28931377 359.00 USD Add to cart
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TC-Clamp 25 mm 44050805 179.15 USD Add to cart
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TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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Door Key, square 44551050 34.08 USD Add to cart
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1. Assemble the Normal flow filter (NFF) inlet to the flow kit.
2. Evacuate air by filling NFF with liquid. Use the system to pump liquid through the system to the NFF.
3. Assemble the NFF to the column inlet. Extra tubing may be needed to prolong the flow kit. Please make sure the tubing is not folded and does not contain any air.

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# Product Name Product Code Price
TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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Clamp, 50 mm TC 28938191 245.00 USD Add to cart
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Gasket 51 mm 28966886 74.16 USD Add to cart
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TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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Gasket 50 mm (1.5") TC i.d. 38 mm, silicone 56410996 88.79 USD Add to cart
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To isolate the column from the system use TC End Cap, 25 mm TC incl. gaskets 18-1001-25 and TC Clamp 25 mm.

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

The ReadyToProcess 32 L (450/200) columns have an extra tube connected to the top outlet. This tube should be used to connect the column to the ÄKTA ready System.

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# Product Name Product Code Price
5 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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6 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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7 Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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The upgrade kit contains UNICORN strategy CD for UNICORN 7 that allows the ÄKTA ready systems to be run with UNICORN 7.0.1 or later versions and the ÄKTA ready Operating Instructions. The UNICORN 7 license need to be bought separately.

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# Product Name Product Code Price
ÄKTA ready Strategy Upgrade Kit 29172736 253.00 USD Add to cart
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Item Comment
1

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

2 For Low Flow Kit use tubing with i.d. 6 mm and for High Flow Kit use tubing with i.d. 9.5 mm (PTFE) or 10 mm (PVC), refer to tables below.
3 TC Gasket 25 mm i.d. 15 mm 44-5492-88, two gaskets are supplied with the flow kit. Two gaskets i.d. 15 mm are needed to connect column to system.
4 TC Clamp 25 mm 28-4043-38 (Stainless steel) or 44-4043-36 (Polypropylene).
5

For Low Flow Kit use 4-port, 2-way Valve, i.d. 6 mm, 25 mm TC 18-5757-01.

For High Flow Kit use 4-port, 2-way Valve i.d. 10 mm, 25 mm TC 18-1012-56.

6

For Low Flow Kit use gasket with i.d. 6 mm 18-0019-27 or i.d. 8 mm 28-9509-48.

For High Flow Kit use gasket with i.d. 10 mm 18-1035-79.

To isolate the column from the system use 4-port, 2-way valves or alternatively only  TC End Cap, 25 mm TC incl. gaskets 18-1001-25  and TC Clamp 25 mm.

 

PTFE tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.5 m 28-4053-68
6 mm 0.8 m 28-4053-69
6 mm 1 m 28-4053-70
6 mm 2 m 28-4053-71
6 mm 4 m 28-4053-72
9.5 mm 0.5 m 28-4053-73
9.5 mm 0.8 m 28-4053-75
9.5 mm 1 m 28-4053-76
9.5 mm 2 m 28-4053-77
9.5 mm 4 m 28-4053-78

 

PVC tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.3 m 18-0005-42
6 mm 0.75 m 18-0005-43
6 mm 1.25 m 18-0005-44
6 mm 1.5 m 18-0005-45
6 mm 2 m 18-0005-47
10 mm 0.3 m 18-1012-85
10 mm 0.4 m 18-1012-86
10 mm 0.9 m 18-1012-62
10 mm 1.4 m 18-1012-63
10 mm 1.7 m 18-1012-64
10 mm 2 m 18-1012-87

 

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# Product Name Product Code Price
2 Tubing, 300 mm, 25 mm TC, i.d. 6 mm 18000542 348.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 6 mm 18000543 372.00 USD Add to cart
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2 Tubing, 1250 mm, 25 mm TC, i.d. 6 mm 18000544 378.00 USD Add to cart
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2 Tubing, 1500 mm, 25 mm TC, i.d. 6 mm 18000545 391.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 6 mm 18000547 438.00 USD Add to cart
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2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 6 mm, 25 mm TC 28405368 468.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 6 mm, 25 mm TC 28405369 491.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 6 mm, 25 mm TC 28405370 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 6 mm, 25 mm TC 28405371 630.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 6 mm, 25 mm TC 28405372 765.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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5 Valve 4-Port 2-Way ID 6 mm 18575701 1 694.00 USD Add to cart
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6 Gasket 25 mm, i.d. 6 mm 18001927 15.42 USD Add to cart
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6 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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6 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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# Product Name Product Code Price
2 pH Electrode 29386679 713.00 USD Add to cart
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3 ÄKTApilot Air Inlet Filter 18116958 130.53 USD Add to cart
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4 Pressure Cell Holder Kit 28929887 415.00 USD Add to cart
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5 Pump Track Assembly 28930915 1 400.00 USD Add to cart
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ÄKTA ready Air Sensor 29003879 2 404.00 USD Add to cart
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Item Comment
1

Low Flow Kit, 28-9301-82 (for flow rates 3 l/h-175 l/h). 

2 Connector 25 mm TC- 5/16" female 18-1169-22. Two connectors needed to connect column to system.
3 TC Gasket 25 mm, i.d. 15 mm 44-5492-88. Two gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect column to system.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene).
5

For appropriate tubing i.d. refer to table below.

6 Stop Plug 5/16" male 18-1112-50.

To isolate the column from the system use Stop Plug 5/16" 28-9819-57.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 50 1.7 mm 5/16"
AxiChrom 70/100 2.9 mm 5/16"

 

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# Product Name Product Code Price
2 Connector, 25 mm TC - UNF 5/16" Female 18116922 176.74 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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Stop plug 5/16" 28981957 79.85 USD Add to cart
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Components of an ÄKTA ready flow kit.

Part number Description
1 Inlet manifold
2 Pump tubing
3 Flow cell for pressure sensor
4 Air trap
5 Air vent tubing
6 Column inlet connection
7 Column outlet connection
8 Flow cell for pressure sensor
9 pH electrode (optional) and has to be ordered separately.
10 Flow meter cell
11 Temperature cell
12 Conductivity cell
13 UV flow cell
14 Flow cell for pressure sensor
15 Outlet manifold

 

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Flow path schemes

The liquid flow through ÄKTA ready is illustrated in the chart below.
(Black: standard components; Red: optional components)

The table below specifies the maximum operating pressure the flow path could be pressurized to with the pressure upgrade strategy in the different parts of the system.

Part Description Pressure
A Low pressure, over the Inlet Manifold 0.6 bar
B High pressure, over the air trap manifold and column 5.0 bar
C Medium pressure 2.0 bar
D Medium pressure, over the outlet manifold 0.95 bar

 

The table below describes the system components shown in the flow chart.

ID tag Function
XV-001 to XV-006 Inlet 1 to 6, grouped in Inlet Manifold
XV-051 to XV-056 Outlet 1 to 6, grouped in Outlet Manifold
PT-111 to PT-113 3 Pressure sensor cells
AE-121 pH electrode (optional)
FE-141 Flow meter cell
TE-161 Temperature sensor
CE-101 Conductivity sensor
AE-131 UV cell
AT-221 Air trap
HV-301 Air trap vent valve
P-201 System pump
P-202 Gradient pump (optional)

 

From inlet to column

Stage Description
1 Inlet valves open the appropriate inlet (XV-001 to XV-006 in chart) for sample
or buffer.
2 The system pump (P-201) and the gradient pump (P-202) deliver the liquid to the column via a pressure sensor cell (PT-111) and, if preferred, via the air trap (AT-221), where air in the liquid is removed.
3

There are two sets of valves between the pump and the column:

  • the air trap manifold, which allows for bypassing the air trap, and
  • the column manifold, which allows for bypassing the column.

 

From column to outlet

Stage Description
1 Downstream of the column, the liquid passes through a second pressure
sensor cell (PT-112), which has an integrated flow cell for a pH electrode
(AE-121).
2

The liquid then continues through:

  • a flow meter cell (FE-141) with integrated temperature sensor (TE-161),
  • a conductivity cell (CE-101), and
  • a UV cell (AE-131).
3 The last sensor in the path is a third pressure sensor (PT-113).
4 Downstream from the sensors, the liquid continues via the outlet tubing to
the outlet manifold, where valves (XV-051 to XV-056) divert the liquid to either
waste or fraction collection.

 

Maximum pressures

  • At the outlet manifold, the system works under a pressure of max 0.95 bar.
  •  Between pump and column the pressure is max 5 bar.
  •  Between column and outlet manifold the pressure is max 2 bar.
  •  The different pressure zones are monitored by pressure sensors PT-111 to PT-113.

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Pressure sensors

Valves

Issues related to Valves.

Conductivity measurement

Issues related to Conductivity measurement

Valves

Issues related to Valves.

Pump

Issues related to the Pump.

Computer

Issues related to the computer.

Power

Issues related to power

Flow meter

Issues related to the Flow meter.

UV measurement

Issues related to UV measurement.

Compressed air

Issues related to compressed air.

Flow kit

Issues related to the Flow kits.

Air in the flow path

Issues related to Air in the flow path.

Contact