Protran and Protran Premium are both unsupported nitrocellulose membranes and can be used for both chemiluminescence and fluorescence detection. Protran Premium is recommended for fluorescence detection systems.
Protran Premium offers a higher protein binding capacity, as shown in the table.
|0.45 µm pore size||0.2 µm pore size|
|Protran||115–125 µg/cm2||150–176 µg/cm2|
|Protran Premium||162–180 µg/cm2||173–203 µg/cm2|
Protran Premium also provides lower background and higher sensitivity and resolution.
Protran and Protran Premium are based on different base nitrocellulose materials.
I used to buy Whatman Protran or Amersham Hybond ECL membrane. How do I know which membrane to buy now?
The Whatman and Amersham products have been combined to offer a single simpler range. The new product names are provided in the table.
|Membrane type||Previous name||New name|
|Nitrocellulose||Whatman Protran BA85||Amersham Protran NC 0.45|
|Whatman Protran BA83||Amersham Protran NC 0.2|
|Whatman Protran BA79||Amersham Protran NC 0.1|
|Amersham Hybond ECL 0.45 µm||Amersham Protran Premium NC 0.45|
|Amersham Hybond ECL 0.2 µm||Amersham Protran Premium NC 0.2|
|Whatman Optitran BAS85||Amersham Protran Supported NC 0.45|
|Whatman Optitran BAS83||Amersham Protran Supported NC 0.2|
|PVDF||Amersham Hybond P||Amersham Hybond PVDF 0.45|
|None||Amersham Hybond PVDF 0.2|
|Amersham Hybond LFP||Amersham Hybond LFP PVDF 0.2|
|Whatman Westran S||Amersham Hybond SEQ PVDF 0.2|