FAQ

Spare parts

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# Product Name Product Code Price
1 Bayonet tool 28401390 91.72 USD Add to cart
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3 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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4 Hook wrench key 28907141 119.72 USD Add to cart
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5 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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6 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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7 Torque Wrench Kit for AxiChrom Columns 28936137 481.00 USD Add to cart
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8 Torque Driver Kit for AxiChrom Columns 28936139 1 117.00 USD Add to cart
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Figure 3. Adaptor assembly (Plastic bed supports).

 

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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102 Guide ring 28932943 310.00 USD Add to cart
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103 Adaptor rod seal 100 28932982 214.00 USD Add to cart
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104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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105 Adaptor rod top 100 28401956 311.00 USD Add to cart
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106 Set screw M4x5 mm 28401976 72.31 USD Add to cart
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107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 98x3 mm 28401967 49.37 USD Add to cart
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109 Tube connection 5/16 id 2.9 Fem/fem 28963351 177.86 USD Add to cart
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111 O-ring 23.52x1.78 mm 28401968 15.18 USD Add to cart
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405 Adaptor tube i.d. 2.9 mm 28981285 215.00 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 100 28401953 959.00 USD Add to cart
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803 Top plate 28978950 1 222.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
127 O-ring 70x2.5 mm 28963306 281.00 USD Add to cart
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301 Bed support AxiChrom 100 adaptor/bottom 20 µm 28981126 456.00 USD Add to cart
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301 Bed support AxiChrom 100 adaptor/bottom, 10 µm 28981127 455.00 USD Add to cart
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401 Screw M4x17 mm 28932986 286.00 USD Add to cart
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402 Distributor 100 top, PE bed support 28981172 915.00 USD Add to cart
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403 Screw M3x25 mm 28933003 175.99 USD Add to cart
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404 Distributor 100 bottom, PE bed support 28981173 907.00 USD Add to cart
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412 Washer 4.3x12.1 mm 28932990 175.99 USD Add to cart
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413 O-ring 3.69x1.78 mm 28932992 224.00 USD Add to cart
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421 Snap ring 100 tip/bottom, PE bed support 28981174 318.00 USD Add to cart
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501 Adaptor plate AxiChrom 100 bed support 28981122 810.00 USD Add to cart
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502 Bottom plate 100, PE bed support 28981123 828.00 USD Add to cart
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604 O-ring 91.67x3.53 mm 28932994 35.25 USD Add to cart
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605 Scraper 28932996 411.00 USD Add to cart
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606 O-ring 88x4 mm 28963312 186.49 USD Add to cart
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Figure 3. Adaptor assembly (stainless steel bed supports).

 

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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102 Guide ring 28932943 310.00 USD Add to cart
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103 Adaptor rod seal 100 28932982 214.00 USD Add to cart
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104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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105 Adaptor rod top 100 28401956 311.00 USD Add to cart
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106 Set screw M4x5 mm 28401976 72.31 USD Add to cart
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107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 98x3 mm 28401967 49.37 USD Add to cart
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109 Tube connection 5/16 id 2.9 Fem/fem 28963351 177.86 USD Add to cart
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111 O-ring 23.52x1.78 mm 28401968 15.18 USD Add to cart
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405 Adaptor tube id 2.9 L=636 28963326 149.67 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 100 28401953 959.00 USD Add to cart
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803 Top plate 28978950 1 222.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
801 Screw M6x20 mm 28933035 171.02 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
131 Stop plug 5/16" 28981957 79.85 USD Add to cart
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701 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
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701 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
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701 Hex socket head screw M8x12 28944567 63.19 USD Add to cart
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Figure 4. Stainless steel bed supports

 

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
127 O-ring 70x2.5 mm 28963306 281.00 USD Add to cart
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301 Bed support adaptor AxiChrom 100, 10 µm 28401981 1 424.00 USD Add to cart
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302 Bed support bottom AxiChrom 100, 10 µm 28932999 1 206.00 USD Add to cart
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302 Bed support bottom AxiChrom 100, 20 µm 28933001 1 135.00 USD Add to cart
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401 Screw M4x17 mm 28932986 286.00 USD Add to cart
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402 End cell 100 28963358 845.00 USD Add to cart
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403 Screw M3x25 mm 28933003 175.99 USD Add to cart
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412 Washer 4.3x12.1 mm 28932990 175.99 USD Add to cart
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413 O-ring 3.69x1.78 mm 28932992 224.00 USD Add to cart
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501 Adaptor plate 100 28978951 815.00 USD Add to cart
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502 Bottom plate 28401948 704.00 USD Add to cart
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604 O-ring 91.67x3.53 mm 28932994 35.25 USD Add to cart
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605 Scraper 28932996 411.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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114 O-ring 104.5x3 mm 28401961 119.84 USD Add to cart
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115 Tube support 28906922 135.32 USD Add to cart
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116 Check valve, inner complete 18116988 322.00 USD Add to cart
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119 Washer 8.4x16x1.5 mm 19078101 60.27 USD Add to cart
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124 Check valve adapter 28401978 116.85 USD Add to cart
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201 Column tube 100/300 short 28963341 2 245.00 USD Add to cart
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201 Column tube 100/500 long 28963347 2 518.00 USD Add to cart
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804 Top bayonet 28978967 1 500.00 USD Add to cart
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805 Bottom flange 28401944 735.00 USD Add to cart
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806 Nut, M8 19076301 196.67 USD Add to cart
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901 Tie bar AxiChrom 100 for short tubes 28401946 253.00 USD Add to cart
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901 Tie bar AxiChrom 100 for long tubes 28401947 296.00 USD Add to cart
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O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. Please keep a set of each on your site to prevent long downtime. For code no refer to below table.

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# Product Name Product Code Price
1 Adaptor rod seal 100 28932982 214.00 USD Add to cart
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2 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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3 O-ring 207x3.4 mm 28942254 100.09 USD Add to cart
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5 O-ring 23.52x1.78 mm 28401968 15.18 USD Add to cart
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6 Check valve, inner complete 18116988 322.00 USD Add to cart
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7 Bed support adaptor AxiChrom 100, 10 µm 28401981 1 424.00 USD Add to cart
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8 Bed support 100 top/20SS 28963333 1 181.00 USD Add to cart
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9 Bed support AxiChrom 100 adaptor/bottom 20 µm 28981126 456.00 USD Add to cart
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10 Bed support bottom AxiChrom 100, 10 µm 28932999 1 206.00 USD Add to cart
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11 Bed support bottom AxiChrom 100, 20 µm 28933001 1 135.00 USD Add to cart
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12 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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13 O-ring 70x2.5 mm 28963306 281.00 USD Add to cart
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14 O-ring 91.67x3.53 mm 28932994 35.25 USD Add to cart
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15 Scraper 28932996 411.00 USD Add to cart
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16 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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Accessories

Figure. Accessories for packing and running AxiChrom 70 and AxiChrom 100 with ÄKTApilot.

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2 and 4 are part of tubing kit.

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# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
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1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
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1 Tubing Kit for Connecting AxiChrom 70 and AxiChrom 100 on Floor to ÄKTApilot, i.d. 2.9 mm 28913614 392.00 USD Add to cart
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5 Valve One Straight Channel, 5/16" 28907674 968.00 USD Add to cart
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6 Vent valve 28960875 87.44 USD Add to cart
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7 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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8 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
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9 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
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10 AxiChrom foot 70 28401937 459.00 USD Add to cart
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11 Mechanical Locking for AxiChrom 70 28401840 679.00 USD Add to cart
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11 Mechanical Locking for AxiChrom 100 28401841 992.00 USD Add to cart
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12 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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12 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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13 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
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13 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
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14 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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15 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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16 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
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17 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
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18 Connector, 25 mm TC - UNF 5/16" Male 18116923 164.99 USD Add to cart
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19 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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20 Clamp, 25 mm TC 28938190 120.54 USD Add to cart
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21 Domed Nut, M6 18245001 47.45 USD Add to cart
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22 Media Stirrer, 80 mm diameter 28919103 214.00 USD Add to cart
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22 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
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23 Torque Wrench Kit for AxiChrom Columns 28936137 481.00 USD Add to cart
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24 Torque Driver Kit for AxiChrom Columns 28936139 1 117.00 USD Add to cart
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Item Comment
1

Low Flow Kit, 28-9301-82 (for flow rates 3 l/h-175 l/h). 

2 Connector 25 mm TC- 5/16" female 18-1169-22. Two connectors needed to connect column to system.
3 TC Gasket 25 mm, i.d. 15 mm 44-5492-88. Two gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect column to system.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene).
5

For appropriate tubing i.d. refer to table below.

6 Stop Plug 5/16" male 18-1112-50.

To isolate the column from the system use Stop Plug 5/16" 28-9819-57.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 50 1.7 mm 5/16"
AxiChrom 70/100 2.9 mm 5/16"

 

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# Product Name Product Code Price
2 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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Stop plug 5/16" 28981957 79.85 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Drifting baseline

Worrying peak shape

Poor reproducibility

Column leakage

Strange peaks

Back pressure increases during operation

Unusual column appearance

Unusual column appearance

Poor product recovery

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact