FAQ

Spare parts

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
114 O-ring 203x3 mm 28942252 206.00 USD Add to cart
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115 Tube support AxiChrom 200 column 28942250 386.00 USD Add to cart
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118 Check valve and housing, TC25 28983704 232.00 USD Add to cart
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119 Washer 10.5x20 mm 28942132 138.87 USD Add to cart
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124 Check valve adapter 28401978 116.85 USD Add to cart
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201 Glass tube short 28941905 4 158.00 USD Add to cart
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201 Glass tube long 28941907 5 572.00 USD Add to cart
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804 Top flange AxiChrom 200 column 28983690 1 582.00 USD Add to cart
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805 Bottom flange AxiChrom 200 column 28941901 1 863.00 USD Add to cart
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806 Nut, M10 18101244 22.67 USD Add to cart
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901 Extender rod for short column tube AxiChrom 28941865 621.00 USD Add to cart
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901 Extender rod for long column tube AxiChrom 28941868 643.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
102 Guide ring AxiChrom 140 and 200 columns 28942042 90.82 USD Add to cart
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103 Top gasket AxiChrom 140 and 200 columns 28942039 89.54 USD Add to cart
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104 O-ring 26.64x2.62 mm 28942110 18.68 USD Add to cart
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105 Adaptor rod top AxiChrom 140 and 200 columns 28942034 472.00 USD Add to cart
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106 Set screw M4x5 mm 28401976 72.31 USD Add to cart
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107 Purge valve kit AxiChrom 140 and 200 28942102 238.00 USD Add to cart
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108 O-ring 207x3.4 mm 28942254 100.09 USD Add to cart
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110 Adaptor tube AxiChrom 200 column 28941935 1 471.00 USD Add to cart
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111 O-ring 76 x 4 mm EPDM 28942108 79.61 USD Add to cart
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602 O-ring 7.3x2.4 mm 28950950 94.11 USD Add to cart
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802 Adaptor rod AxiCh 140/200 28985789 1 251.00 USD Add to cart
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803 Top plate for AxiChrom 200 28983677 1 674.00 USD Add to cart
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807 Hex socket head screw M6x14 28942256 193.81 USD Add to cart
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# Product Name Product Code Price
1 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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2 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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3 Torque Wrench Kit for AxiChrom Columns 28936137 481.00 USD Add to cart
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4 Torque Driver Kit for AxiChrom Columns 28936139 1 117.00 USD Add to cart
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5 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. Please keep a set of each on your site to prevent long downtime. For code numbers refer to the table below.

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# Product Name Product Code Price
1 Top gasket AxiChrom 140 and 200 columns 28942039 89.54 USD Add to cart
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2 O-ring 26.64x2.62 mm 28942110 18.68 USD Add to cart
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3 O-ring 207x3.4 mm 28942254 100.09 USD Add to cart
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4 Adaptor tube AxiChrom 200 column 28941935 1 471.00 USD Add to cart
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5 O-ring 76 x 4 mm EPDM 28942108 79.61 USD Add to cart
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6 Fastener kit adaptor AxiChrom 200 column 28942260 209.00 USD Add to cart
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7 Check valve and housing, TC25 28983704 232.00 USD Add to cart
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8 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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10 Bed support adaptor AxiChrom 200, 10 µm 28942014 5 182.00 USD Add to cart
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11 Bed support adaptor AxiChrom 200, 20 µm 28942016 5 005.00 USD Add to cart
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12 Bed support 200 top/bottom 20 mikrom PE 28981558 947.00 USD Add to cart
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13 Bed support bottom AxiChrom 200, 10 µm 28942018 5 182.00 USD Add to cart
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14 Bed support bottom AxiChrom 200, 20 µm 28942020 5 132.00 USD Add to cart
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15 O-ring 7.3x2.4 mm 28950950 94.11 USD Add to cart
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16 O-ring 158.5x3 mm 28942029 110.38 USD Add to cart
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17 O-ring 184.3x5.7 mm 28942027 110.38 USD Add to cart
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18 Scraper AxiChrom 200 column 28942258 432.00 USD Add to cart
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20 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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21 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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Figure 4. Stainless steel bed supports

 

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
112 O-ring 3.69x1.78 mm 28932992 224.00 USD Add to cart
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112 Fastener kit adaptor AxiChrom 200 column 28942260 209.00 USD Add to cart
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301 Bed support adaptor AxiChrom 200, 10 µm 28942014 5 182.00 USD Add to cart
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301 Bed support adaptor AxiChrom 200, 20 µm 28942016 5 005.00 USD Add to cart
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302 Bed support bottom AxiChrom 200, 10 µm 28942018 5 182.00 USD Add to cart
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302 Bed support bottom AxiChrom 200, 20 µm 28942020 5 132.00 USD Add to cart
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402 End cell top AxiChrom 200 column 28942022 1 508.00 USD Add to cart
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403 Hex socket head screw M4x35 28942123 199.52 USD Add to cart
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404 End cell bottom AxiChrom 200 column 28942024 1 574.00 USD Add to cart
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501 Adaptor plate AxiChrom 200 28985792 620.00 USD Add to cart
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502 Bottom plate AxiChrom 200 column 28941894 1 537.00 USD Add to cart
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603 O-ring 158.5x3 mm 28942029 110.38 USD Add to cart
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604 O-ring 184.3x5.7 mm 28942027 110.38 USD Add to cart
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605 Scraper AxiChrom 200 column 28942258 432.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
125 Washer 8.4x16 mm 28942138 197.99 USD Add to cart
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801 Hex socket head screw M8x20 mm 28942116 196.88 USD Add to cart
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808 Hex head screw M8x20 28942119 196.73 USD Add to cart
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Figure 4. Plastic bed supports

 

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
127 O-ring 158.5x3 mm 28942029 110.38 USD Add to cart
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301 Bed support 200 top/bottom 20 mikrom PE 28981558 947.00 USD Add to cart
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301 Bed support 200 top/bottom 10 mikrom UHMWPE 28981559 960.00 USD Add to cart
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401 O-ring 3.69x1.78 mm 28932992 224.00 USD Add to cart
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401 Fastener kit adaptor AxiChrom 200 column 28942260 209.00 USD Add to cart
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402 Distributor top, PE bed supports 28981536 1 409.00 USD Add to cart
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403 Hex socket head screw M4x35 28942123 199.52 USD Add to cart
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501 Adaptor plate AxiChrom 200 28985792 620.00 USD Add to cart
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502 Bottom plate AxiChrom 200 column 28941894 1 537.00 USD Add to cart
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604 O-ring 184.3x5.7 mm 28942027 110.38 USD Add to cart
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605 Scraper AxiChrom 200 column 28942258 432.00 USD Add to cart
Get Quote

Please note:

Item numbers in gray illustrates parts in contact with process stream/or that are pressure retaining.

Item numbers are not necessarily in sequential order.

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# Product Name Product Code Price
130 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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131 TC end cap 28966791 324.00 USD Add to cart
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132 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
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132 Connector, 25 mm TC - UNF 5/16" Male, short 18117008 169.94 USD Add to cart
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608 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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610 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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702 Hex socket head screw M8x12 28944567 63.19 USD Add to cart
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Accessories

Figure. Tubing kits and connections for ÄKTApilot.

Table. Tubing kits and connections for ÄKTApilot.

 

Pos. Tubing kit AxiChrom 200, i.d. 4.8 Tubing connections
1 i.d. 6.4 / L=1600

Connect tubing 1 from the column top to the position 3 on valve 7 (V7)

2 i.d. 6.4 / L=1600 Connect tubing 2 from hydraulic chamber to position 1 on valve 7 (V7)
3 i.d. 6.4 / L=1600 Connect tubing 3 from the column bottom to position 3 on valve 6 (V6)
4 i.d. 6.4 / L=2000  
5

TC Gasket 25mm i.d. 6.5 mm1 

(10 x Gasket supplied with the tubing kit)

 
6

TC Gasket 25mm i.d. 8.0 mm2 

(2 x Gasket supplied with the tubing kit)

 
7

25 mm TC Clamp3 

(7 x Clamp supplied with the tubing kit)

 
8 Connector TC 5/16" short, supplied with the tubing kit  

1 Use if at least one of the connecting ends is made of plastic.
2 Use if both connecting ends are made of metal.
3 Use plastic clamp if at least one of the connecting ends is made of plastic.

Note: The tubing internal diameters for connection between column and ÄKTApilot are the same as for ÄKTAprocess.

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# Product Name Product Code Price
1 Silicone tubing kit AxiChrom 140, i.d. 4.8 mm ÄKTApilot 28977958 2 786.00 USD Add to cart
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1 Silicone tubing kit AxiChrom 140, i.d. 3.2 mm ÄKTApilot 28977960 2 343.00 USD Add to cart
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1 Silicone tubing kit AxiChrom 200, i.d. 6.4 mm ÄKTApilot 28977970 1 949.00 USD Add to cart
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5 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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6 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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7 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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7 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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Accessories for connecting AxiChrom 200 to ÄKTAprocess system

 

Figure. Tubing kits and connections for ÄKTAprocess.

Table. Tubing kit and connections for ÄKTAprocess.

 

Pos. Tubing kit AxiChrom 200, i.d. 3.2 Tubing kit AxiChrom 200, i.d. 6.4 Tubing connections
1 i.d. 3.2 / L=2000 i.d. 6.4 / L=2000

Inlet CIP1 ÄKTAprocess 

-> Column top mobile phase inlet/outlet

2 i.d. 3.2 / L=1000 i.d. 6.4 / L=1000

ÄKTAprocess Column bottom 

-> Column bottom mobile phase inlet/outlet

3 i.d. 3.2 / L=1600 i.d. 6.4 / L=1600

Inlet CIP2 

-> Column check valve

4 i.d. 3.2 / L=2000 i.d. 6.4 / L=2000 Column hydraulic chamber outlet -> waste
5

TC Gasket 25mm i.d. 6.5 mm1 

(10 x Gasket supplied with the tubing kit)

 
6

TC Gasket 25mm i.d. 8.0 mm2 

(10 x Gasket supplied with the tubing kit)

 
7

25 mm TC Clamp3 

(7 x Clamp supplied with the tubing kit)

 

1 Use if at least one of the connecting ends is made of plastic.

2 When both ends are made of metal stronger force can be applied to the clamp and therefore reduce the original inner diameter of the gasket. We recommend you to use the gasket with larger inner diameter if both connecting ends are made of metal. 

3 Use plastic clamp if at least one of the connecting ends is made of plastic, clamp 25 mm TC in polypropylene, 28-4043-36.

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# Product Name Product Code Price
1 Tubing Kit i.d. 3.2 mm for Connecting AxiChrom 140 to ÄKTAprocess, silicone 28942986 2 089.00 USD Add to cart
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1 Tubing Kit i.d. 6.4 mm for Connecting AxiChrom 200 to ÄKTAprocess, silicone 28943020 2 099.00 USD Add to cart
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1 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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2 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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6 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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7 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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7 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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TC-Gaskets 25/6.5 mm 44549286 162.40 USD Add to cart
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TC-Gaskets 25/8.0 mm 44549289 202.00 USD Add to cart
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Item Comment
1

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

2 For appropriate tubing i.d. refer to table below.
3 TC Gasket 25 mm i.d. 15 mm 44-5492-88, gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect the column to the flow kit.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene). Four clamps needed to connect to system.
5

TC Gasket Kit 25 mm, i.d. 6.5 mm, 28-9667-37. Two gaskets i.d. 6.5 mm needed to connect the column to the system.

6 TC End Cap, 25 mm TC, 28-9667-91.
7 Connector, 25 mm TC - UNF 5/16" Male, short, 18-1170-08.
8 O-Ring, 3 × 1 mm 18-1169-12.

To isolate the column from the system use 2-port, 2-way Valve i.d. 6 mm, 44-5501-00 (left) or 44-5501-01 (right) or alternatively only TC End Cap, 25 mm TC, 28-9667-91, TC Clamp 25 mm and a gasket. Please verify that the pack sizes of clamps and gaskets correspond to your needs.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 140 3.2 mm1 or 4.8 mm2 25 mm TC
AxiChrom 200 6.4 mm 25 mm TC

1 It is recommended to use tubing with id 3.2 mm for media with an approximate bead size < 50 µm.

2 For media with an approximate bead size > 50 µm it is recommended to use tubing with id 4.8 mm.

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# Product Name Product Code Price
3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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6 TC end cap 28966791 324.00 USD Add to cart
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7 Connector, 25 mm TC - UNF 5/16" Male, short 18117008 169.94 USD Add to cart
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8 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
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2 port, 2 way valves i.d. 6 mm (left) 44550100 418.00 USD Add to cart
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2 port, 2 way valves i.d. 6 mm (right) 44550101 409.00 USD Add to cart
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# Product Name Product Code Price
1 Silicone tubing kit AxiChrom 140, i.d. 3.2 mm ÄKTApilot 28977960 2 343.00 USD Add to cart
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2 Silicone tubing kit AxiChrom 140, i.d. 4.8 mm ÄKTApilot 28977958 2 786.00 USD Add to cart
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3 Silicone tubing kit AxiChrom 200, i.d. 6.4 mm ÄKTApilot 28977970 1 949.00 USD Add to cart
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4 6 bar rupture disc kit for AxiChrom 200 28978006 2 156.00 USD Add to cart
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5 8 bar rupture disc kit for AxiChrom 140 28978012 2 160.00 USD Add to cart
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6 Rupture disc 6 bar, TC50 28978183 1 744.00 USD Add to cart
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7 Rupture disc 8 bar, TC50 28978184 1 537.00 USD Add to cart
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# Product Name Product Code Price
1 Mechanical Locking, AxiChrom 200 28943353 1 290.00 USD Add to cart
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2 Tubing Kit i.d. 6.4 mm for Connecting AxiChrom 200 to ÄKTAprocess, silicone 28943020 2 099.00 USD Add to cart
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3 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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4 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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5 TC end cap 28966791 324.00 USD Add to cart
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6 Media Stirrer, 80 mm diameter 28919103 214.00 USD Add to cart
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7 Media Stirrer,150 mm diameter 28919104 253.00 USD Add to cart
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8 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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9 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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10 Torque Wrench Kit for AxiChrom Columns 28936137 481.00 USD Add to cart
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11 Torque Driver Kit for AxiChrom Columns 28936139 1 117.00 USD Add to cart
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12 Outlet Tubing Kit, AxiChrom 140 and 200 columns 28943041 647.00 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Drifting baseline

Worrying peak shape

Poor reproducibility

Column leakage

Strange peaks

Back pressure increases during operation

Unusual column appearance

Unusual column appearance

Poor product recovery

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact