FAQ

Spare parts

Stainless steel bed support kit 20 µm (28-9664-47) and related parts.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
1 Bed Support kit AxiChrom 50, 20 µm SS 28966447 1 953.00 USD Add to cart
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Screw M3x16 mm 28901467 26.83 USD Add to cart
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Bed support 50 Bottom/20SS 28963228 814.00 USD Add to cart
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Bed support 50 top/20SS 28963415 885.00 USD Add to cart
Get Quote

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
301 Bed support 50 top/bottom 20mikrom PE 28988320 577.00 USD Add to cart
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301 Bed support 50 top/bottom 10mikrom UHMWPE 28988322 577.00 USD Add to cart
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401 Set screw M3x12.5 mm special 28988329 61.38 USD Add to cart
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402 Distributor 50 top, PE bed support 28988312 570.00 USD Add to cart
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403 Screw M3x16 mm 28901467 26.83 USD Add to cart
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404 Distributor 50 top, PE bed support 28988312 570.00 USD Add to cart
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409 O-ring 28.3x1.78 EPDM 28963367 172.84 USD Add to cart
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412 Washer 3.2x9x0.8 mm 28988461 24.83 USD Add to cart
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413 O-ring 2.5 x 1.3 EPDM 28988459 49.97 USD Add to cart
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421 Snap ring 50 top/bottom, PE bed support 28988325 379.00 USD Add to cart
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422 Support net 50, PE bed support 28988334 78.54 USD Add to cart
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501 Adaptor plate AxiChrom 50 28987619 632.00 USD Add to cart
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502 Bottom plate 28988309 504.00 USD Add to cart
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604 O-ring 44.12x2.62 EPDM 28963385 95.67 USD Add to cart
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605 Scraper 50 UHMWPE 28963373 405.00 USD Add to cart
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606 O-ring 44.2x3 FPM/FKM 28963209 184.12 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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114 O-ring 54x2 mm 28401771 234.00 USD Add to cart
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115 Tube support 50 28901444 211.00 USD Add to cart
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116 Check valve holder 28401767 251.00 USD Add to cart
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117 Check valve 18110213 97.28 USD Add to cart
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118 Check valve 18110213 97.28 USD Add to cart
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119 Washer M6 6.4x12x2 mm 28901462 21.27 USD Add to cart
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201 Column tube 50/300, Short 28963237 1 588.00 USD Add to cart
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201 Column tube 50/500 long 28963243 1 803.00 USD Add to cart
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804 Top bayonet AxiChrom 50 28978948 881.00 USD Add to cart
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805 Bottom flange for AxiChrom 50 28401744 370.00 USD Add to cart
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806 Nut M6 18112648 32.79 USD Add to cart
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901 Tie bar short 28401760 144.94 USD Add to cart
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901 Tie bar long 28401796 156.80 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
102 O-ring 15.45x2.8 mm 28401775 39.70 USD Add to cart
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104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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105 Adaptor rod top 50 28401766 302.00 USD Add to cart
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106 Set screw M3x6 28401790 44.00 USD Add to cart
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107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 48x2.5 mm 28401776 23.38 USD Add to cart
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109 Tube connection 5/16 i.d. 1.7 mm fem/fem 28963186 185.24 USD Add to cart
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111 O-ring 11.1x1.6 mm 28401778 27.82 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 50 28401801 614.00 USD Add to cart
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803 Top plate AxiChrom 50 28978940 882.00 USD Add to cart
Get Quote

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
102 O-ring 15.45x2.8 mm 28401775 39.70 USD Add to cart
Get Quote
104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
Get Quote
105 Adaptor rod top 50 28401766 302.00 USD Add to cart
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106 Set screw M3x6 28401790 44.00 USD Add to cart
Get Quote
107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 48x2.5 mm 28401776 23.38 USD Add to cart
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109 Tube connection 5/16 i.d. 1.7 mm fem/fem 28963186 185.24 USD Add to cart
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111 O-ring 11.1x1.6 mm 28401778 27.82 USD Add to cart
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405 Tube id 1.7 cpl, PE bed support 28988332 397.00 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 50 28401801 614.00 USD Add to cart
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803 Top plate AxiChrom 50 28978940 882.00 USD Add to cart
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# Product Name Product Code Price
123 Foot for AxiChrom 50 column 28401794 499.00 USD Add to cart
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131 Stop plug 5/16" 28981957 79.85 USD Add to cart
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Figure 4. Spare parts, Stainless steel bed supports.

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
301 Bed support adaptor 10 µm 28924562 1 262.00 USD Add to cart
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301 Bed support 50 top/20SS 28963415 885.00 USD Add to cart
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302 Bed support bottom 10 µm 28401849 741.00 USD Add to cart
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302 Bed support 50 Bottom/20SS 28963228 814.00 USD Add to cart
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401 Screw M3x8 mm 28901470 36.74 USD Add to cart
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403 Screw M3x16 mm 28901467 26.83 USD Add to cart
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501 Adaptor plate AxiChrom 50, 20 µm bedsupport 28963437 1 429.00 USD Add to cart
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501 Adaptor AxiChrom 50 10microm 28966755 1 409.00 USD Add to cart
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502 Bottom Plate AxiChrom 50, 20 µm Bed Support 28963077 1 384.00 USD Add to cart
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502 Bottom plate AxiChrom 50, 10 µm bedsupport 28966775 1 409.00 USD Add to cart
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603 O-ring 28.3x1.78 EPDM 28963367 172.84 USD Add to cart
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604 O-ring 44.12x2.62 EPDM 28963385 95.67 USD Add to cart
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605 Scraper 50 UHMWPE 28963373 405.00 USD Add to cart
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606 O-ring 44.2x3 FPM/FKM 28963209 184.12 USD Add to cart
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607 O-ring 34.646x1.78 EPDM 28963194 100.97 USD Add to cart
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Bed Support kit AxiChrom 50, 20 µm SS 28966447 1 953.00 USD Add to cart
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Bed Support kit AxiChrom 50, 10 µm SS 28966449 2 010.00 USD Add to cart
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# Product Name Product Code Price
1 Bayonet tool 28401390 91.72 USD Add to cart
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2 Hook wrench key 28907141 119.72 USD Add to cart
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3 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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4 Centering tool 50 28401886 294.00 USD Add to cart
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5 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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6 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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Stainless steel bed support kit 10 µm (28-9664-49) and related parts.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
1 Bed Support kit AxiChrom 50, 10 µm SS 28966449 2 010.00 USD Add to cart
Get Quote
Bed support bottom 10 µm 28401849 741.00 USD Add to cart
Get Quote
Screw M3x16 mm 28901467 26.83 USD Add to cart
Get Quote
Bed support adaptor 10 µm 28924562 1 262.00 USD Add to cart
Get Quote
Adaptor AxiChrom 50 10microm 28966755 1 409.00 USD Add to cart
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Bottom plate AxiChrom 50, 10 µm bedsupport 28966775 1 409.00 USD Add to cart
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# Product Name Product Code Price
801 Screw M4 x 16 18110956 53.65 USD Add to cart
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O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. Please keep a set of each on your site to prevent long downtime. For code numbers refer to the table below.

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# Product Name Product Code Price
1 Tube connection 5/16 i.d. 1.7 mm fem/fem 28963186 185.24 USD Add to cart
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2 Adaptor tube id 1.7 L=637 28963219 149.23 USD Add to cart
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3 Bed support adaptor 10 µm 28924562 1 262.00 USD Add to cart
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4 Bed support 50 top/20SS 28963415 885.00 USD Add to cart
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5 Bed support bottom 10 µm 28401849 741.00 USD Add to cart
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6 Bed support 50 Bottom/20SS 28963228 814.00 USD Add to cart
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7 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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8 O-ring 28.3x1.78 EPDM 28963367 172.84 USD Add to cart
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9 O-ring 44.12x2.62 EPDM 28963385 95.67 USD Add to cart
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10 Scraper 50 UHMWPE 28963373 405.00 USD Add to cart
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11 O-ring 44.2x3 FPM/FKM 28963209 184.12 USD Add to cart
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12 O-ring 34.646x1.78 EPDM 28963194 100.97 USD Add to cart
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13 Vent valve 28960875 87.44 USD Add to cart
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14 Check valve 18110213 97.28 USD Add to cart
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15 Foot for AxiChrom 50 column 28401794 499.00 USD Add to cart
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Accessories

Figure 2. Accessories for packing and running AxiChrom 50 column with ÄKTApilot when column is placed on floor and other accessories.

 

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2 and 4 are part of tubing kit.

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# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
Get Quote
1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
Get Quote
1 Tubing Kit for Connecting AxiChrom 50 and AxiChrom 70 on Floor to ÄKTApilot, i.d. 1.7 mm 28913613 391.00 USD Add to cart
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5 Valve One Straight Channel, 5/16" 28907674 968.00 USD Add to cart
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6 Vent valve 28960875 87.44 USD Add to cart
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7 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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8 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
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9 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
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10 Mechanical Locking for AxiChrom 50 28401839 485.00 USD Add to cart
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11 Tool kit AxiChrom 50 28404777 595.00 USD Add to cart
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12 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
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13 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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14 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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15 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
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16 Domed Nut, M6 18245001 47.45 USD Add to cart
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17 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
Get Quote

Figure 1. Accessories for packing and running AxiChrom 50  with ÄKTAexplorer when column and system are placed on desk and other accessories.

 

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2, 3 and 7 are part of tubing kit.

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# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
Get Quote
1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
Get Quote
1 Tubing Kit for Connecting AxiChrom 50 on Bench to ÄKTAexplorer 28905541 308.00 USD Add to cart
Get Quote
8 Valve SRV-1 19214501 242.00 USD Add to cart
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9 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
Get Quote
10 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
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11 Vent valve 28960875 87.44 USD Add to cart
Get Quote
12 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
13 Stop plug 19517001 38.34 USD Add to cart
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14 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
Get Quote
15 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
Get Quote
16 Mechanical Locking for AxiChrom 50 28401839 485.00 USD Add to cart
Get Quote
17 Tool kit AxiChrom 50 28404777 595.00 USD Add to cart
Get Quote
18 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
Get Quote
19 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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20 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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21 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
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22 Domed Nut, M6 18245001 47.45 USD Add to cart
Get Quote
23 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
Get Quote

Figure 2. Accessories for packing and running AxiChrom 50 with ÄKTAexplorer when column is placed on floor and other accessories.

 

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2, 3 and 7 are part of tubing kit.

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# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
Get Quote
1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
Get Quote
1 Tubing Kit for Connecting AxiChrom 50 on Floor to ÄKTAexplorer 28905603 306.00 USD Add to cart
Get Quote
8 Valve SRV-1 19214501 242.00 USD Add to cart
Get Quote
9 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
Get Quote
10 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
Get Quote
11 Vent valve 28960875 87.44 USD Add to cart
Get Quote
12 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
13 Stop plug 19517001 38.34 USD Add to cart
Get Quote
14 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
Get Quote
15 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
Get Quote
16 Mechanical Locking for AxiChrom 50 28401839 485.00 USD Add to cart
Get Quote
17 Tool kit AxiChrom 50 28404777 595.00 USD Add to cart
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18 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
Get Quote
19 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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20 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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21 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
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22 Domed Nut, M6 18245001 47.45 USD Add to cart
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23 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
Get Quote

The illustrations show tubing connections for ÄKTA avant 150.

The illustration to the left refers to Tubing kit, AxiChrom 50 -70 / ÄKTA avant / desk, 28-9888-89.

The illustration to the right refers to Tubing kit, AxiChrom 50 - 70 / ÄKTA avant / floor, 28-9888-92.

Pos.

28-9888-89 Tubing kit, 

AxiChrom 50 - 70 / ÄKTA avant / desk

28-9888-92 Tubing kit, 

AxiChrom 50 - 70 / ÄKTA avant / floor

1 i.d. 1.7 / L=1200 (x2) i.d. 1.7 / L=1600 (x2)
2 i.d. 1.7 / L=800 (x1) i.d. 1.7 / L=1200 (x1)
3 i.d. 2.9 / L=1600 (x1) i.d. 2.9 / L=1600 (x1)
4 i.d. 1.7 / L=200 (x2) i.d. 1.7 / L=200 (x2)

 

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# Product Name Product Code Price
Tubing kit AxiChrom 50-70 AKTA avant, desk 28988889 513.00 USD Add to cart
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Tubing kit AxiChrom 50-70 AKTA avant, floor 28988892 519.00 USD Add to cart
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Item Comment
1

Low Flow Kit, 28-9301-82 (for flow rates 3 l/h-175 l/h). 

2 Connector 25 mm TC- 5/16" female 18-1169-22. Two connectors needed to connect column to system.
3 TC Gasket 25 mm, i.d. 15 mm 44-5492-88. Two gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect column to system.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene).
5

For appropriate tubing i.d. refer to table below.

6 Stop Plug 5/16" male 18-1112-50.

To isolate the column from the system use Stop Plug 5/16" 28-9819-57.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 50 1.7 mm 5/16"
AxiChrom 70/100 2.9 mm 5/16"

 

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# Product Name Product Code Price
2 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
Stop plug 5/16" 28981957 79.85 USD Add to cart
Get Quote

Figure 1. Accessories for packing and running AxiChrom 50 with ÄKTApilot when column is placed on desk and other accessories.

 

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2 and 4 are part of tubing kit.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
Get Quote
1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
Get Quote
1 Tubing Kit for connecting AxiChrom 50 on bench to ÄKTApilot 28905676 336.00 USD Add to cart
Get Quote
5 Valve One Straight Channel, 5/16" 28907674 968.00 USD Add to cart
Get Quote
6 Vent valve 28960875 87.44 USD Add to cart
Get Quote
7 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
Get Quote
8 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
Get Quote
9 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
Get Quote
10 Mechanical Locking for AxiChrom 50 28401839 485.00 USD Add to cart
Get Quote
11 Tool kit AxiChrom 50 28404777 595.00 USD Add to cart
Get Quote
12 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
Get Quote
13 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
Get Quote
14 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
Get Quote
15 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
Get Quote
16 Domed Nut, M6 18245001 47.45 USD Add to cart
Get Quote
17 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
Get Quote

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Drifting baseline

Worrying peak shape

Poor reproducibility

Column leakage

Strange peaks

Back pressure increases during operation

Unusual column appearance

Unusual column appearance

Poor product recovery

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact