FAQ

Spare parts

Figure 4. Stainless steel bed supports.

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
301 Bed support adaptor AxiChrom 70, 10 µm 28924574 1 379.00 USD Add to cart
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301 Bed support 70 top/20SS 28963430 1 255.00 USD Add to cart
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302 Bed support bottom AxiChrom 70, 10 µm 28401931 792.00 USD Add to cart
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302 Bed support 70 bottom/20 SS 28963267 920.00 USD Add to cart
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401 Screw M3x8 mm 28901470 36.74 USD Add to cart
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403 Screw M3x16 mm 28901467 26.83 USD Add to cart
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501 Adaptor plate AxiChrom 70, 20 µm Bed Support 28963433 1 421.00 USD Add to cart
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501 Adaptor AxiChrom 70 10microm 28966770 1 442.00 USD Add to cart
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502 Bottom plate AxiChrom 70, 20 µm Bed support 28963222 1 484.00 USD Add to cart
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502 Bottom plate AxiChrom 70 10microm 28966779 1 517.00 USD Add to cart
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603 O-ring 47x2 EPDM 28963418 131.55 USD Add to cart
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604 Scraper and O-ring AxiChrom 70 28977257 350.00 USD Add to cart
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606 O-ring 63.09x3.53 mm 28963261 95.01 USD Add to cart
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607 O-ring 54x2 mm 28963254 210.00 USD Add to cart
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Bed Support kit AxiChrom 70, 20 µm SS 28966446 2 060.00 USD Add to cart
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Bed Support kit AxiChrom 70, 10 µm SS 28966448 2 288.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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102 O-ring 19.5x3 mm 28401919 27.77 USD Add to cart
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104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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105 Adaptor rod top 28401900 212.00 USD Add to cart
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106 Set screw M3x6 28401790 44.00 USD Add to cart
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107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 65x3 mm 28401920 27.82 USD Add to cart
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109 Tube connection 5/16 id 2.9 Fem/fem 28963351 177.86 USD Add to cart
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111 O-ring 14x1.5 mm 28401922 23.98 USD Add to cart
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405 Tube i.d. 2.9 mm complete 28988333 396.00 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 28401817 679.00 USD Add to cart
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803 Top plate 28978949 1 002.00 USD Add to cart
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Figure 3. Stainless steel bed supports

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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102 O-ring 19.5x3 mm 28401919 27.77 USD Add to cart
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104 O-ring 10.3x2.4 mm 28401783 20.50 USD Add to cart
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105 Adaptor rod top 28401900 212.00 USD Add to cart
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106 Set screw M3x6 28401790 44.00 USD Add to cart
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107 Vent valve 28960875 87.44 USD Add to cart
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108 O-ring 65x3 mm 28401920 27.82 USD Add to cart
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109 Tube connection 5/16 id 2.9 Fem/fem 28963351 177.86 USD Add to cart
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111 O-ring 14x1.5 mm 28401922 23.98 USD Add to cart
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126 Vent valve 28960875 87.44 USD Add to cart
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405 Tube i.d. 2.9 mm complete 28988333 396.00 USD Add to cart
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602 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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802 Adaptor rod 28401817 679.00 USD Add to cart
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803 Top plate 28978949 1 002.00 USD Add to cart
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# Product Name Product Code Price
1 Bayonet tool 28401390 91.72 USD Add to cart
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2 Hook wrench key 28907141 119.72 USD Add to cart
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3 Mounting aid for O-rings 28924749 13.55 USD Add to cart
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4 Centering tool 28401903 283.00 USD Add to cart
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5 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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6 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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Figure 4. Spare parts, Plastic bed supports.

Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
301 Bed support 70 top/bottom 10mikrom UHMWPE 28988338 576.00 USD Add to cart
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301 Bed support 70 top/bottom 20mikrom PE 28988341 579.00 USD Add to cart
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401 Set screw M3x12.5 mm special 28988329 61.38 USD Add to cart
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402 Distributor 70 top, PE bed support 28988344 847.00 USD Add to cart
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403 Screw M3x16 mm 28901467 26.83 USD Add to cart
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404 Distributor 70 bottom, PE bed support 28988345 872.00 USD Add to cart
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409 O-ring 47x2 EPDM 28963418 131.55 USD Add to cart
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412 Washer 3.2x9x0.8 mm 28988461 24.83 USD Add to cart
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413 O-ring 2.5 x 1.3 EPDM 28988459 49.97 USD Add to cart
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421 Snap ring 70 top/bottom, PE bed support 28988328 412.00 USD Add to cart
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422 Support net 70, PE bed support 28988335 78.38 USD Add to cart
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501 Adaptor plate 28988337 716.00 USD Add to cart
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502 Bottom plate 70 28988343 729.00 USD Add to cart
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604 Scraper and O-ring AxiChrom 70 28977257 350.00 USD Add to cart
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606 O-ring 63.09x3.53 mm 28963261 95.01 USD Add to cart
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Scraper and O-ring AxiChrom 70 28977257 350.00 USD Add to cart
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Please note:

Item numbers in gray illustrates parts in contact with process stream.

Item numbers are not necessarily in sequential order.

Important: AxiChrom stainless steel and plastic bed supports are not directly interexchangable.

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# Product Name Product Code Price
101 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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114 O-ring 72x2 mm 28401914 29.23 USD Add to cart
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115 Tube support 28901442 204.00 USD Add to cart
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116 Check valve holder 28401767 251.00 USD Add to cart
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117 Check valve 18110213 97.28 USD Add to cart
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119 Washer M6 6.4x12x2 mm 28901462 21.27 USD Add to cart
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201 Column tube 70/300 short 28963300 1 728.00 USD Add to cart
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201 Column tube 70/500 long 28963303 2 081.00 USD Add to cart
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804 Top bayonet 28978966 1 134.00 USD Add to cart
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805 Bottom flange 28401798 582.00 USD Add to cart
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806 Nut M6 18112648 32.79 USD Add to cart
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901 Tie bar short 28401760 144.94 USD Add to cart
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901 Tie bar long 28401796 156.80 USD Add to cart
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Bed support kit 10 µm (28-9664-48) and related parts.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
1 Bed Support kit AxiChrom 70, 10 µm SS 28966448 2 288.00 USD Add to cart
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Bed support bottom AxiChrom 70, 10 µm 28401931 792.00 USD Add to cart
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Screw M3x16 mm 28901467 26.83 USD Add to cart
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O-ring 63.17x2.62 mm for piston scraper 28924572 169.24 USD Add to cart
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Bed support adaptor AxiChrom 70, 10 µm 28924574 1 379.00 USD Add to cart
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Adaptor AxiChrom 70 10microm 28966770 1 442.00 USD Add to cart
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Bottom plate AxiChrom 70 10microm 28966779 1 517.00 USD Add to cart
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Bed support kit 20 µm (28-9664-46) and related parts.

Important: The 10 and 20 µm bed supports are not interexchangable. The entire bed support kit, that is the bottom plate and top assembly, must be exchanged.

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# Product Name Product Code Price
1 Bed Support kit AxiChrom 70, 20 µm SS 28966446 2 060.00 USD Add to cart
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Screw M3x16 mm 28901467 26.83 USD Add to cart
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O-ring 63.17x2.62 mm for piston scraper 28924572 169.24 USD Add to cart
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# Product Name Product Code Price
131 Stop plug 5/16" 28981957 79.85 USD Add to cart
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701 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
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701 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
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# Product Name Product Code Price
801 Screw M4 x 16 18110956 53.65 USD Add to cart
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# Product Name Product Code Price
2 Adaptor tube id 2.9 L=636 28963326 149.67 USD Add to cart
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3 Bed support adaptor AxiChrom 70, 10 µm 28924574 1 379.00 USD Add to cart
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4 Bed support 70 top/20SS 28963430 1 255.00 USD Add to cart
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5 Bed support bottom AxiChrom 70, 10 µm 28401931 792.00 USD Add to cart
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6 Bed support 70 bottom/20 SS 28963267 920.00 USD Add to cart
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7 O-ring 3x1 EPDM 28963214 31.44 USD Add to cart
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8 O-ring 47x2 EPDM 28963418 131.55 USD Add to cart
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9 Scraper and O-ring AxiChrom 70 28977257 350.00 USD Add to cart
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10 Scraper and O-ring AxiChrom 70 28977257 350.00 USD Add to cart
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11 O-ring 63.09x3.53 mm 28963261 95.01 USD Add to cart
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12 O-ring 54x2 mm 28963254 210.00 USD Add to cart
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13 Vent valve 28960875 87.44 USD Add to cart
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14 Check valve 18110213 97.28 USD Add to cart
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Accessories

Figure. Accessories for packing and running AxiChrom 70 and AxiChrom 100 with ÄKTApilot.

Please note:

Item numbers are not necessarily in sequential order.

Items 1, 2 and 4 are part of tubing kit.

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# Product Name Product Code Price
1 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
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1 ÄKTApilot O-Ring, 3 × 1 mm 18116912 661.00 USD Add to cart
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1 Tubing Kit for Connecting AxiChrom 70 and AxiChrom 100 on Floor to ÄKTApilot, i.d. 2.9 mm 28913614 392.00 USD Add to cart
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5 Valve One Straight Channel, 5/16" 28907674 968.00 USD Add to cart
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6 Vent valve 28960875 87.44 USD Add to cart
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7 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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8 Pivot Stand for AxiChrom 50/70/100-300 28401709 1 613.00 USD Add to cart
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9 Pivot stand for AxiChrom 50/70/100-500 28401710 1 662.00 USD Add to cart
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10 AxiChrom foot 70 28401937 459.00 USD Add to cart
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11 Mechanical Locking for AxiChrom 70 28401840 679.00 USD Add to cart
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11 Mechanical Locking for AxiChrom 100 28401841 992.00 USD Add to cart
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12 Tool kit small AxiChrom 50–200 28936136 549.00 USD Add to cart
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12 Tool Kit Complete for AxiChrom 50–200 Columns 28944261 1 637.00 USD Add to cart
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13 Union, M6 Female - 5/16" Male 18116916 105.47 USD Add to cart
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13 Union, M6 Female - 5/16" Female 18116917 89.33 USD Add to cart
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14 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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15 Connector 5/16" Female - M6 Male 18112776 55.00 USD Add to cart
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16 Union 5/16" female - 1/16" male 18114208 47.45 USD Add to cart
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17 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
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18 Connector, 25 mm TC - UNF 5/16" Male 18116923 164.99 USD Add to cart
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19 TC-gasket kit 25 mm i.d. 6.5 mm 28966737 47.19 USD Add to cart
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20 Clamp, 25 mm TC 28938190 120.54 USD Add to cart
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21 Domed Nut, M6 18245001 47.45 USD Add to cart
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22 Media Stirrer, 80 mm diameter 28919103 214.00 USD Add to cart
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22 Media Stirrer, 40 mm diameter 28923180 383.00 USD Add to cart
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23 Torque Wrench Kit for AxiChrom Columns 28936137 481.00 USD Add to cart
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24 Torque Driver Kit for AxiChrom Columns 28936139 1 117.00 USD Add to cart
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The illustrations show tubing connections for ÄKTA avant 150.

The illustration to the left refers to Tubing kit, AxiChrom 50 -70 / ÄKTA avant / desk, 28-9888-89.

The illustration to the right refers to Tubing kit, AxiChrom 50 - 70 / ÄKTA avant / floor, 28-9888-92.

Pos.

28-9888-89 Tubing kit, 

AxiChrom 50 - 70 / ÄKTA avant / desk

28-9888-92 Tubing kit, 

AxiChrom 50 - 70 / ÄKTA avant / floor

1 i.d. 1.7 / L=1200 (x2) i.d. 1.7 / L=1600 (x2)
2 i.d. 1.7 / L=800 (x1) i.d. 1.7 / L=1200 (x1)
3 i.d. 2.9 / L=1600 (x1) i.d. 2.9 / L=1600 (x1)
4 i.d. 1.7 / L=200 (x2) i.d. 1.7 / L=200 (x2)

 

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# Product Name Product Code Price
Tubing kit AxiChrom 50-70 AKTA avant, desk 28988889 513.00 USD Add to cart
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Tubing kit AxiChrom 50-70 AKTA avant, floor 28988892 519.00 USD Add to cart
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Item Comment
1

Low Flow Kit, 28-9301-82 (for flow rates 3 l/h-175 l/h). 

2 Connector 25 mm TC- 5/16" female 18-1169-22. Two connectors needed to connect column to system.
3 TC Gasket 25 mm, i.d. 15 mm 44-5492-88. Two gaskets supplied with the flow kit. Two gaskets i.d. 15 mm needed to connect column to system.
4 TC Clamp 25 mm, 28-4043-38 (Stainless steel) or 28-4043-36 (Polypropylene).
5

For appropriate tubing i.d. refer to table below.

6 Stop Plug 5/16" male 18-1112-50.

To isolate the column from the system use Stop Plug 5/16" 28-9819-57.

 

Tubing

Column Tubing i.d. Tubing connection
AxiChrom 50 1.7 mm 5/16"
AxiChrom 70/100 2.9 mm 5/16"

 

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# Product Name Product Code Price
2 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 30.19 USD Add to cart
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Stop plug 5/16" 28981957 79.85 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Drifting baseline

Worrying peak shape

Poor reproducibility

Column leakage

Strange peaks

Back pressure increases during operation

Unusual column appearance

Unusual column appearance

Poor product recovery

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact