FAQ

Accessories

Figure. BPG 300, Accessories for Packing the column, parts identification.

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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3 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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6 Manometer Kit 0-6 Bar 28994472 2 203.00 USD Add to cart
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7 Pressure relief valve i.d. 10 mm, 25 mm TC 18110697 1 617.00 USD Add to cart
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8 Safety Valve, 4 bar 18103582 3 278.00 USD Add to cart
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9 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 772.00 USD Add to cart
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10 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 499.00 USD Add to cart
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10 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 410.00 USD Add to cart
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10 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 598.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 2 141.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 355.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 462.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 3 211.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 4 067.00 USD Add to cart
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11 Gasket 51 mm 28966886 74.16 USD Add to cart
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11 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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12 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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13 Packing Device, BPG 300 Column 18110816 11 800.00 USD Add to cart
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Torque Wrench for BPG Columns, 2-20 N 18025137 470.00 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Allen Key for BPG Columns 18103098 33.15 USD Add to cart
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12-Point Opening Socket for BPG 300 Column 18103105 81.69 USD Add to cart
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Media Stirrer,150 mm diameter 28919104 278.00 USD Add to cart
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Figure. BPG 300, Accessories for Running the column, parts identification.

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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3 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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6 Valve, 4-port, 4-way, i.d. 10 mm 18101257 2 162.00 USD Add to cart
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7 Manometer Kit 0-6 Bar 28994472 2 203.00 USD Add to cart
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8 Air Trap Complete 18110298 9 825.00 USD Add to cart
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9 Safety Valve, 4 bar 18103582 3 278.00 USD Add to cart
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10 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 772.00 USD Add to cart
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11 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 499.00 USD Add to cart
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11 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 410.00 USD Add to cart
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11 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 598.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 2 141.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 355.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 462.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 3 211.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 4 067.00 USD Add to cart
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12 Gasket 51 mm 28966886 74.16 USD Add to cart
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12 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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13 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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14 Top Valve for Air Traps 18112144 1 510.00 USD Add to cart
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16 Filter 215 nm 11000733 744.00 USD Add to cart
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16 Filter 260 nm 11000734 710.00 USD Add to cart
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16 Filter 280 nm 11000735 519.00 USD Add to cart
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16 Filter 405 nm 11000736 679.00 USD Add to cart
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16 Empty Filter Holder 11000738 222.00 USD Add to cart
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16 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 9 825.00 USD Add to cart
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16 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
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16 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
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16 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
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16 O-ring kit 28969705 133.83 USD Add to cart
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17 Flow cell i.d. 10 mm, 25 mm TC 18100065 3 716.00 USD Add to cart
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17 Filter Kit 280 nm 19243301 983.00 USD Add to cart
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Torque Wrench for BPG Columns, 2-20 N 18025137 470.00 USD Add to cart
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Allen Key for BPG Columns 18103098 33.15 USD Add to cart
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12-Point Opening Socket for BPG 300 Column 18103105 81.69 USD Add to cart
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Grounding kit 18115787 191.20 USD Add to cart
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Item Comment
1

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

2 For Low Flow Kit use tubing with i.d. 6 mm and for High Flow Kit use tubing with i.d. 9.5 mm (PTFE) or 10 mm (PVC), refer to tables below.
3 TC Gasket 25 mm i.d. 15 mm 44-5492-88, two gaskets are supplied with the flow kit. Two gaskets i.d. 15 mm are needed to connect column to system.
4 TC Clamp 25 mm 28-4043-38 (Stainless steel) or 44-4043-36 (Polypropylene).
5

For Low Flow Kit use 4-port, 2-way Valve, i.d. 6 mm, 25 mm TC 18-5757-01.

For High Flow Kit use 4-port, 2-way Valve i.d. 10 mm, 25 mm TC 18-1012-56.

6

For Low Flow Kit use gasket with i.d. 6 mm 18-0019-27 or i.d. 8 mm 28-9509-48.

For High Flow Kit use gasket with i.d. 10 mm 18-1035-79.

To isolate the column from the system use 4-port, 2-way valves or alternatively only  TC End Cap, 25 mm TC incl. gaskets 18-1001-25  and TC Clamp 25 mm.

 

PTFE tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.5 m 28-4053-68
6 mm 0.8 m 28-4053-69
6 mm 1 m 28-4053-70
6 mm 2 m 28-4053-71
6 mm 4 m 28-4053-72
9.5 mm 0.5 m 28-4053-73
9.5 mm 0.8 m 28-4053-75
9.5 mm 1 m 28-4053-76
9.5 mm 2 m 28-4053-77
9.5 mm 4 m 28-4053-78

 

PVC tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.3 m 18-0005-42
6 mm 0.75 m 18-0005-43
6 mm 1.25 m 18-0005-44
6 mm 1.5 m 18-0005-45
6 mm 2 m 18-0005-47
10 mm 0.3 m 18-1012-85
10 mm 0.4 m 18-1012-86
10 mm 0.9 m 18-1012-62
10 mm 1.4 m 18-1012-63
10 mm 1.7 m 18-1012-64
10 mm 2 m 18-1012-87

 

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# Product Name Product Code Price
2 Tubing, 300 mm, 25 mm TC, i.d. 6 mm 18000542 348.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 6 mm 18000543 372.00 USD Add to cart
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2 Tubing, 1250 mm, 25 mm TC, i.d. 6 mm 18000544 378.00 USD Add to cart
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2 Tubing, 1500 mm, 25 mm TC, i.d. 6 mm 18000545 391.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 6 mm 18000547 438.00 USD Add to cart
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2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 6 mm, 25 mm TC 28405368 468.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 6 mm, 25 mm TC 28405369 491.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 6 mm, 25 mm TC 28405370 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 6 mm, 25 mm TC 28405371 630.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 6 mm, 25 mm TC 28405372 765.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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5 Valve 4-Port 2-Way ID 6 mm 18575701 1 694.00 USD Add to cart
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6 Gasket 25 mm, i.d. 6 mm 18001927 15.42 USD Add to cart
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6 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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6 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Spare parts

Figure 2. BPG 300 Column parts identification.

Item numbers are not necessarily in sequential order. For parts shown in the figure but not listed below, please contact your sales or service representative.

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# Product Name Product Code Price
13 Distribution plate BPG 300 18101237 121.64 USD Add to cart
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15 Gasket Kit TC 25 ID 12mm EPDM 28966741 33.75 USD Add to cart
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15 TC gasket 25/10.5 mm FPM/FKM 28969519 620.00 USD Add to cart
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15 TC-gasket 25/10.5 mm EPDM 44549287 172.06 USD Add to cart
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23 Seal adjuster 18101241 953.00 USD Add to cart
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24 Adaptor height adjuster 18112831 670.00 USD Add to cart
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25 Bushing ring 18101242 119.96 USD Add to cart
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26 Adaptor Top Plate BPG 300 18112906 2 605.00 USD Add to cart
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27 Nut, M10 18101244 22.67 USD Add to cart
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28 Stop screw, complete 18101243 209.00 USD Add to cart
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29 Adjuster nut BPG 300 18101245 2 138.00 USD Add to cart
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30 Sealing Unit BPG 300 18114749 1 296.00 USD Add to cart
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31 Adaptor head screw, M8 18101246 74.85 USD Add to cart
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32 Allen screw, M8 x 60 18101247 35.85 USD Add to cart
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33 O-ring, 274 × 8 EPDM Adaptor 18101251 103.59 USD Add to cart
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33 O-ring Adaptor 274 × 8 FEP 18101252 205.00 USD Add to cart
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35 Inner adaptor tube BPG 300 18114783 303.00 USD Add to cart
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36 Outer adaptor tube BPG 300 18114293 2 021.00 USD Add to cart
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39 Adaptor Complete BPG 300 18111282 1 659.00 USD Add to cart
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40 Support net adaptor BPG 300 18101253 81.36 USD Add to cart
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41 Net 23 μm adaptor BPG 300 PP 18101254 282.00 USD Add to cart
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41 Net 10 μm adaptor BPG 300 PA 18101255 381.00 USD Add to cart
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41 Net 54 μm adaptor BPG 300 PP 18112702 288.00 USD Add to cart
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41 Net 12 μm adaptor BPG 300 PEEK 18114843 681.00 USD Add to cart
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44 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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48 Stop screw, complete 18101243 209.00 USD Add to cart
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49 Stop screw, complete 18101243 209.00 USD Add to cart
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52 Seal adjuster 18101241 953.00 USD Add to cart
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53 Adaptor height adjuster 18112831 670.00 USD Add to cart
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O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets.

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for code numbers refer to the table below.

The column is delivered with O-rings in Ethylene propylene diene (EPDM). Ethylene propylene diene change characteristics when exposed to some organic chemicals. For repetitive long term use, use Fluoroethenepropene (FEP) polymers.

23 µm nets are supplied with the column and recommended for beads with an average particle diameter between 70-150 µm. For an average particle diameter < 70 µm use 10 µm or 12 µm nets and for an average particle diameter > 150 µm use 54 µm nets.

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# Product Name Product Code Price
1 O-ring, 301 × 6 EPDM 18101226 89.76 USD Add to cart
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2 O-ring, 301 × 6 FEP 18101227 180.57 USD Add to cart
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3 O-ring, 274 × 8 EPDM Adaptor 18101251 103.59 USD Add to cart
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4 O-ring Adaptor 274 × 8 FEP 18101252 205.00 USD Add to cart
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5 Support net adaptor BPG 300 18101253 81.36 USD Add to cart
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6 Support net endpiece BPG 300 18101236 75.37 USD Add to cart
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7 Net 10 μm adaptor BPG 300 PA 18101255 381.00 USD Add to cart
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8 Net 10 μm endpiece BPG 300 PA 18101235 373.00 USD Add to cart
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9 Net 12 μm adaptor BPG 300 PEEK 18114843 681.00 USD Add to cart
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10 Net 12 μm endpiece BPG 300 PEEK 18114844 761.00 USD Add to cart
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11 Net 23 μm adaptor BPG 300 PP 18101254 282.00 USD Add to cart
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12 Net 23 μm endpiece BPG 300 PP 18101234 269.00 USD Add to cart
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13 Net 54 μm adaptor BPG 300 PP 18112702 288.00 USD Add to cart
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14 Net 54 μm endpiece BPG 300 PP 18112703 441.00 USD Add to cart
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15 Distribution plate BPG 300 18101237 121.64 USD Add to cart
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Item numbers are not necessarily in sequential order. For parts shown in the figure but not listed below, please contact your sales or service representative.

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# Product Name Product Code Price
2 Nut, M12 18101224 29.66 USD Add to cart
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3 Washer 13 x 24 x 2.5 18101261 116.70 USD Add to cart
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3 Adaptor Top Plate/Flange BPG 300 29269256 5 512.00 USD Add to cart
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5 Adaptor Top Plate/Flange BPG 300 29269256 5 512.00 USD Add to cart
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6 O-ring, 301 × 6 EPDM 18101226 89.76 USD Add to cart
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6 O-ring, 301 × 6 FEP 18101227 180.57 USD Add to cart
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8 Tie rod BPG 300/500 18101231 152.65 USD Add to cart
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8 Tie rod BPG 300/750 18101232 107.64 USD Add to cart
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8 Tie rod BPG 300/950 18101233 180.75 USD Add to cart
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10 Net 23 μm endpiece BPG 300 PP 18101234 269.00 USD Add to cart
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10 Net 10 μm endpiece BPG 300 PA 18101235 373.00 USD Add to cart
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10 Net 54 μm endpiece BPG 300 PP 18112703 441.00 USD Add to cart
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10 Net 12 μm endpiece BPG 300 PEEK 18114844 761.00 USD Add to cart
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12 Support net endpiece BPG 300 18101236 75.37 USD Add to cart
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13 Distribution plate BPG 300 18101237 121.64 USD Add to cart
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14 Endpiece BPG 300 18114047 2 417.00 USD Add to cart
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15 Gasket Kit TC 25 ID 12mm EPDM 28966741 33.75 USD Add to cart
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15 TC gasket 25/10.5 mm FPM/FKM 28969519 620.00 USD Add to cart
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15 TC-gasket 25/10.5 mm EPDM 44549287 172.06 USD Add to cart
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18 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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19 Stand, complete 18101238 1 664.00 USD Add to cart
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20 Washer 13 x 24 x 2.5 18101261 116.70 USD Add to cart
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21 Bolt, M12 x 25 18101265 112.05 USD Add to cart
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44 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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47 Adaptor Top Plate/Flange BPG 300 29269256 5 512.00 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact