FAQ

Accessories

Figure. BPG 450, Accessories for Packing the column, parts identification.

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 14 mm 18102728 393.00 USD Add to cart
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2 Tubing, 1800 mm, 25 mm TC, i.d. 14 mm 18102729 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 12 mm 18020000 30.84 USD Add to cart
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3 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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3 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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3 Gasket, 25 mm i.d. 12 mm 44550620 382.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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5 Shutter Valve 3-port, 2-way, i.d. 15.75 mm, TC 25 44549990 4 317.00 USD Add to cart
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6 Manometer Kit 0-6 Bar 28994472 2 203.00 USD Add to cart
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7 Pressure relief valve i.d. 10 mm, 25 mm TC 18110697 1 617.00 USD Add to cart
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8 Safety Valve, BPG 450 18110365 2 999.00 USD Add to cart
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9 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 772.00 USD Add to cart
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10 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 499.00 USD Add to cart
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10 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 410.00 USD Add to cart
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10 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 598.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 2 141.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 355.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 462.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 3 211.00 USD Add to cart
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10 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 4 067.00 USD Add to cart
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11 Gasket 51 mm 28966886 74.16 USD Add to cart
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11 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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12 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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13 Packing Device, BPG 450 Column 18110532 12 300.00 USD Add to cart
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Torque Wrench for BPG Columns, 2-20 N 18025137 470.00 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Allen Key for BPG Columns 18103098 33.15 USD Add to cart
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12-Point Opening Socket for BPG 450 18110531 120.76 USD Add to cart
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Media Stirrer,150 mm diameter 28919104 278.00 USD Add to cart
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Item Comment
1

For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.

For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

2 For Low Flow Kit use tubing with i.d. 6 mm and for High Flow Kit use tubing with i.d. 9.5 mm (PTFE) or 10 mm (PVC), refer to tables below.
3 TC Gasket 25 mm i.d. 15 mm 44-5492-88, two gaskets are supplied with the flow kit. Two gaskets i.d. 15 mm are needed to connect column to system.
4 TC Clamp 25 mm 28-4043-38 (Stainless steel) or 44-4043-36 (Polypropylene).
5

For Low Flow Kit use 4-port, 2-way Valve, i.d. 6 mm, 25 mm TC 18-5757-01.

For High Flow Kit use 4-port, 2-way Valve i.d. 10 mm, 25 mm TC 18-1012-56.

6

For Low Flow Kit use gasket with i.d. 6 mm 18-0019-27 or i.d. 8 mm 28-9509-48.

For High Flow Kit use gasket with i.d. 10 mm 18-1035-79.

To isolate the column from the system use 4-port, 2-way valves or alternatively only  TC End Cap, 25 mm TC incl. gaskets 18-1001-25  and TC Clamp 25 mm.

 

PTFE tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.5 m 28-4053-68
6 mm 0.8 m 28-4053-69
6 mm 1 m 28-4053-70
6 mm 2 m 28-4053-71
6 mm 4 m 28-4053-72
9.5 mm 0.5 m 28-4053-73
9.5 mm 0.8 m 28-4053-75
9.5 mm 1 m 28-4053-76
9.5 mm 2 m 28-4053-77
9.5 mm 4 m 28-4053-78

 

PVC tubing with 25 mm TC

Inner Diameter Length Code Number
6 mm 0.3 m 18-0005-42
6 mm 0.75 m 18-0005-43
6 mm 1.25 m 18-0005-44
6 mm 1.5 m 18-0005-45
6 mm 2 m 18-0005-47
10 mm 0.3 m 18-1012-85
10 mm 0.4 m 18-1012-86
10 mm 0.9 m 18-1012-62
10 mm 1.4 m 18-1012-63
10 mm 1.7 m 18-1012-64
10 mm 2 m 18-1012-87

 

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# Product Name Product Code Price
2 Tubing, 300 mm, 25 mm TC, i.d. 6 mm 18000542 348.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 6 mm 18000543 372.00 USD Add to cart
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2 Tubing, 1250 mm, 25 mm TC, i.d. 6 mm 18000544 378.00 USD Add to cart
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2 Tubing, 1500 mm, 25 mm TC, i.d. 6 mm 18000545 391.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 6 mm 18000547 438.00 USD Add to cart
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2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 6 mm, 25 mm TC 28405368 468.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 6 mm, 25 mm TC 28405369 491.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 6 mm, 25 mm TC 28405370 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 6 mm, 25 mm TC 28405371 630.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 6 mm, 25 mm TC 28405372 765.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 TC-Gasket 25 mm i.d. 15 mm 44549288 169.54 USD Add to cart
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4 TC clamp kit, 25 mm TC 28404336 245.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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5 Valve 4-Port 2-Way ID 6 mm 18575701 1 694.00 USD Add to cart
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6 Gasket 25 mm, i.d. 6 mm 18001927 15.42 USD Add to cart
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6 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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6 TC-gasket 25/8 EPDM 28950948 61.81 USD Add to cart
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Blind Flange, including gasket, 25 mm 18100125 76.96 USD Add to cart
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Figure. BPG 450, Accessories for Running the column, parts identification.

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 344.00 USD Add to cart
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2 Tubing, 400 mm, 25 mm TC i.d. 10 mm 18101286 352.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 14 mm 18102728 393.00 USD Add to cart
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2 Tubing, 1800 mm, 25 mm TC, i.d. 14 mm 18102729 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 12 mm 18020000 30.84 USD Add to cart
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3 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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3 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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3 Gasket, 25 mm i.d. 12 mm 44550620 382.00 USD Add to cart
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4 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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5 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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5 Shutter Valve 3-port, 2-way, i.d. 15.75 mm, TC 25 44549990 4 317.00 USD Add to cart
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6 Valve, 4-port, 4-way, i.d. 10 mm 18101257 2 162.00 USD Add to cart
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7 Manometer Kit 0-6 Bar 28994472 2 203.00 USD Add to cart
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8 Air Trap Complete 18110300 9 449.00 USD Add to cart
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9 Safety Valve, BPG 450 18110365 2 999.00 USD Add to cart
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10 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 772.00 USD Add to cart
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11 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 499.00 USD Add to cart
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11 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 410.00 USD Add to cart
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11 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 598.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 2 141.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 355.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 462.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 3 211.00 USD Add to cart
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11 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 4 067.00 USD Add to cart
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12 Gasket 51 mm 28966886 74.16 USD Add to cart
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12 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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13 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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14 Top Valve for Air Traps 18112144 1 510.00 USD Add to cart
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16 Filter 215 nm 11000733 744.00 USD Add to cart
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16 Filter 260 nm 11000734 710.00 USD Add to cart
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16 Filter 280 nm 11000735 519.00 USD Add to cart
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16 Filter 405 nm 11000736 679.00 USD Add to cart
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16 Empty Filter Holder 11000738 222.00 USD Add to cart
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16 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 9 825.00 USD Add to cart
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16 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
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16 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
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16 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
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16 O-ring kit 28969705 133.83 USD Add to cart
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17 Flow cell i.d. 10 mm, 25 mm TC 18100065 3 716.00 USD Add to cart
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17 Filter Kit 280 nm 19243301 983.00 USD Add to cart
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Torque Wrench for BPG Columns, 2-20 N 18025137 470.00 USD Add to cart
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Allen Key for BPG Columns 18103098 33.15 USD Add to cart
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12-Point Opening Socket for BPG 450 18110531 120.76 USD Add to cart
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Grounding kit 18115787 191.20 USD Add to cart
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Spare parts

O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets.

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for code numbers refer to the table below.

The column is delivered with O-rings in Ethylene propylene diene (EPDM). Ethylene propylene diene change characteristics when exposed to some organic chemicals. For repetitive long term use, use Fluoroethenepropene (FEP) polymers and Perfluor rubber polymers (PFR).

23 µm nets are supplied with the column and recommended for beads with an average particle diameter between 70-150 µm. For an average particle diameter < 70 µm use 10 µm or 12 µm nets and for an average particle diameter > 150 µm use 54 µm nets.

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# Product Name Product Code Price
1 O-ring 446 × 6 EPDM Flange 18110533 125.68 USD Add to cart
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2 O-ring 446 × 6 FEP BPG 450 18111767 271.00 USD Add to cart
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3 O-ring 419 × 10 EPDM Adaptor 18101747 105.90 USD Add to cart
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4 O-ring 419 × 10 FEP BPG 450 18111766 298.00 USD Add to cart
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5 U-shaped seal BPG 450 EPDM 18110440 118.60 USD Add to cart
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7 Support net adaptor BPG 450 18110434 83.82 USD Add to cart
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8 Support net endpiece BPG 450 18110435 84.23 USD Add to cart
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9 Net 10 μm adaptor PA BPG 450 18101746 425.00 USD Add to cart
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10 Net 10 μm endpiece BPG 450 PA 18110318 1 290.00 USD Add to cart
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11 Net 12 μm PEEK adaptor BPG 450 18114845 859.00 USD Add to cart
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12 Net 12 μm endpiece BPG 450 PEEK 18114846 949.00 USD Add to cart
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13 Net 23 μm adaptor BPG 450 18100162 351.00 USD Add to cart
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14 Net 23 μm endpiece BPG 450 PP 18110319 802.00 USD Add to cart
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15 Net 54 μm adaptor BPG 450 18112704 339.00 USD Add to cart
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16 Net 54 μm endpiece BPG 450 PP 18112705 1 411.00 USD Add to cart
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Figure. BPG 450, column parts identification.

Item numbers are not necessarily in sequential order. For parts shown in the figure but not listed below, please contact your sales or service representative.

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# Product Name Product Code Price
1 Endpiece BPG 450 18110967 5 581.00 USD Add to cart
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2 Support net endpiece BPG 450 18110435 84.23 USD Add to cart
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3 Flange BPG 450 18110968 7 791.00 USD Add to cart
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4 U-shaped seal BPG 450 EPDM 18110440 118.60 USD Add to cart
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4 U-Shaped Seal FPM/FKM BPG 450 28992187 7 748.00 USD Add to cart
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5 Net 10 μm endpiece BPG 450 PA 18110318 1 290.00 USD Add to cart
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5 Net 23 μm endpiece BPG 450 PP 18110319 802.00 USD Add to cart
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5 Net 54 μm endpiece BPG 450 PP 18112705 1 411.00 USD Add to cart
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5 Net 12 μm endpiece BPG 450 PEEK 18114846 949.00 USD Add to cart
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6 O-ring 446 × 6 EPDM Flange 18110533 125.68 USD Add to cart
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6 O-ring 446 × 6 FEP BPG 450 18111767 271.00 USD Add to cart
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7 Allen screw M8 × 50 BPG 450 18110940 146.27 USD Add to cart
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8 Tie Rod BPG 450/750 18110309 137.42 USD Add to cart
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8 Tie Rod BPG 450/1000 18110310 117.98 USD Add to cart
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8 Tie Rod BPG 450/500 18110618 164.32 USD Add to cart
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9 Sealing unit BPG 450 18110969 3 587.00 USD Add to cart
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10 Pin bolt BPG 450 18300107 147.44 USD Add to cart
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11 Stop screw M5 x 6 19436301 204.00 USD Add to cart
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12 Glass tube 450/500 18110314 17 200.00 USD Add to cart
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12 Glass tube 450/750 18110315 28 000.00 USD Add to cart
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12 Glass tube 450/1000 18110316 26 900.00 USD Add to cart
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13 Revolving stop BPG 450 18110743 88.49 USD Add to cart
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14 Revolving stop 18110941 350.00 USD Add to cart
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15 Allen screw M8 × 50 BPG 450 18110940 146.27 USD Add to cart
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15 Allen screw M8 × 50 BPG 450 18110940 146.27 USD Add to cart
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16 Spacer BPG 450 18110942 316.00 USD Add to cart
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17 Lid BPG 450 18110970 4 235.00 USD Add to cart
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18 Bushing ring BPG 450 18110943 69.23 USD Add to cart
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19 Adaptor height adjuster BPG 450 18110944 305.00 USD Add to cart
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20 Handle BPG 450 18110945 108.82 USD Add to cart
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21 Adjuster Nut Flange BPG 450 18110946 3 001.00 USD Add to cart
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22 Adaptor stopper BPG 450 18111518 128.46 USD Add to cart
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23 Seal adjuster BPG 450 18110947 1 746.00 USD Add to cart
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24 Handle BPG 450 18110948 106.06 USD Add to cart
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25 Knob BPG 450 18110949 35.85 USD Add to cart
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26 Screw M10 × 65 BPG 450 18110950 46.48 USD Add to cart
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27 Domed nut M10 and washer 18110320 46.57 USD Add to cart
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27 Washer 10.5 × 20 BPG 450 18110951 169.78 USD Add to cart
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28 Domed nut M10 and washer 18110320 46.57 USD Add to cart
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29 Outer Adaptor screw BPG 450 18110954 4 720.00 USD Add to cart
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30 Adjustment tube BPG 450 18110955 439.00 USD Add to cart
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31 Inlet tube BPG 450 18110953 552.00 USD Add to cart
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32 O-ring 12.3 × 2.4 EPDM 18110370 67.84 USD Add to cart
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32 O-ring 12.3 × 2.4 FEP BPG 450 18111765 119.68 USD Add to cart
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35 Screw M5 × 10 BPG 450 18110957 109.57 USD Add to cart
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36 Snap plug BPG 450 18110745 135.92 USD Add to cart
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38 Adaptor plate BPG 450 18110971 4 290.00 USD Add to cart
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40 O-ring 419 × 10 EPDM Adaptor 18101747 105.90 USD Add to cart
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40 O-ring 419 × 10 FEP BPG 450 18111766 298.00 USD Add to cart
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41 Distribution Plate BPG 450 18110604 1 708.00 USD Add to cart
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42 Support net adaptor BPG 450 18110434 83.82 USD Add to cart
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43 Net 23 μm adaptor BPG 450 18100162 351.00 USD Add to cart
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43 Net 10 μm adaptor PA BPG 450 18101746 425.00 USD Add to cart
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43 Net 54 μm adaptor BPG 450 18112704 339.00 USD Add to cart
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43 Net 12 μm PEEK adaptor BPG 450 18114845 859.00 USD Add to cart
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45 Screw, M10 x 16 18110960 109.75 USD Add to cart
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46 Bracket BPG 450 18110961 179.26 USD Add to cart
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47 Bracket BPG 450 18110961 179.26 USD Add to cart
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47 Screw M5 × 16 BPG 450 18110962 110.29 USD Add to cart
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48 Bottom outlet tube BPG 450 18110963 620.00 USD Add to cart
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49 Eye bolt BPG 450 18110964 188.40 USD Add to cart
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50 Stand Assy BPG 450 29018717 550.00 USD Add to cart
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51 Stand Assy BPG 450 29018717 550.00 USD Add to cart
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62 Washer 8.4x16x1.6 A4 18111329 54.14 USD Add to cart
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69 TC Clamp kit TC25/SS 28404338 120.33 USD Add to cart
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71 TC-gasket 25/10.5 mm EPDM 44549287 172.06 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact