FAQ

Spare parts

Item numbers with a white background are not available as standard spare parts.

Please note that item numbers are not necessarily in sequential order.

Tools are not present in the figure.

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# Product Name Product Code Price
1 Seal kit, nozzle man/auto for Chromaflow 400 Small Flow Path 11001388 408.00 USD Add to cart
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2 Nozzle tip, with O-rings mounted 18116106 1 131.00 USD Add to cart
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7 Tool, Bed support retaining nut 11001391 2 228.00 USD Add to cart
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8 Mounting Tool, Nozzle tip seal 18116175 242.00 USD Add to cart
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Item numbers with a white background are not available as standard spare parts.

Please note that item numbers are not necessarily in sequential order.

Tools are not present in the figure.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
6 Bed support retaining screw 11001390 2 819.00 USD Add to cart
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7 Tool, Bed support retaining nut 11001391 2 228.00 USD Add to cart
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8 Mounting Tool, Nozzle tip seal 18116175 242.00 USD Add to cart
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11 Seal kit, Chromaflow 400 Small Flow Path, for 20 µm plastic bed support 11001393 1 655.00 USD Add to cart
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12 Locking rod, Chromaflow 400-800, M20 18116251 346.00 USD Add to cart
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13 Locking rod nut, M20 18116414 47.75 USD Add to cart
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18 Bed support bottom, Chromaflow 400 Small Flow Path, 20µm plastic 11001398 5 088.00 USD Add to cart
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19 Bed support adaptor, Chromaflow 400 Small Flow Path, 20µm plastic 11001399 4 938.00 USD Add to cart
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20 Column tube 400/100-300 18116076 21 500.00 USD Add to cart
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20 Column tube 400/200-400 18116077 24 100.00 USD Add to cart
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20 Column tube 400/300-500 18116078 26 500.00 USD Add to cart
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O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. We recommend you to keep a set of nozzle seals and column seals on your site, for code no refer to table below.

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# Product Name Product Code Price
1 Seal kit, Chromaflow 400 Small Flow Path, for 20 µm plastic bed support 11001393 1 655.00 USD Add to cart
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2 Seal kit, nozzle man/auto for Chromaflow 400 Small Flow Path 11001388 408.00 USD Add to cart
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3 Bed support bottom, Chromaflow 400 Small Flow Path, 20µm plastic 11001398 5 088.00 USD Add to cart
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4 Bed support adaptor, Chromaflow 400 Small Flow Path, 20µm plastic 11001399 4 938.00 USD Add to cart
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5 Nozzle tip, with O-rings mounted 18116106 1 131.00 USD Add to cart
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6 Bed support retaining nut 18116107 537.00 USD Add to cart
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7 Flushing kit sanitary barrier, flushing tube unit 18116108 667.00 USD Add to cart
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Item numbers with a white background are not available as standard spare parts.

Please note that item numbers are not necessarily in sequential order.

Tools are not present in the figure.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
3 Bed support retaining nut for Chromaflow 400 Small Flow Path 11001389 1 510.00 USD Add to cart
Get Quote
4 Flushing kit sanitary barrier, flushing tube unit 18116108 667.00 USD Add to cart
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5 Clamp nut, insert M20 18116109 745.00 USD Add to cart
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7 Tool, Bed support retaining nut 11001391 2 228.00 USD Add to cart
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8 Mounting Tool, Nozzle tip seal 18116175 242.00 USD Add to cart
Get Quote
11 Seal kit, Chromaflow 400 Small Flow Path, for 20 µm plastic bed support 11001393 1 655.00 USD Add to cart
Get Quote

Accessories

Figure. Accessories, Packing and unpacking the Chromaflow 400 SFP Columns with a packing station.

SFP = Small Flow Path

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
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2 Tubing, 750 mm, 25 mm TC, i.d. 14 mm 18102728 393.00 USD Add to cart
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2 Tubing, 1800 mm, 25 mm TC, i.d. 14 mm 18102729 495.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
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3 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 499.00 USD Add to cart
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3 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 410.00 USD Add to cart
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3 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 598.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 2 141.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 355.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 462.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 3 211.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 4 067.00 USD Add to cart
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5 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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5 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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6 Gasket 51 mm 28966886 74.16 USD Add to cart
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6 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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8 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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8 Clamps 25 mm 44056801 155.35 USD Add to cart
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9 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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10 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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10 Valve 2-way, 3-port, i.d. 22 mm, 51 mm TC 44158301 2 374.00 USD Add to cart
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11 Pressure sensor, i.d. 10mm, 25mm TC 28934323 4 554.00 USD Add to cart
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12 Safety valve, 3 bar, 51 mm TC 18573801 3 090.00 USD Add to cart
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14 Castors assembly kit 18117151 3 928.00 USD Add to cart
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15 Nozzle Piping, Chromaflow 400, i.d. 1/2", 51 mm TC 18117201 3 055.00 USD Add to cart
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Figure. Running Chromafow 400 SFP Columns.

 

SFP = Small Flow Path

Item numbers are not necessarily in sequential order

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 364.00 USD Add to cart
Get Quote
1 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 382.00 USD Add to cart
Get Quote
1 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 408.00 USD Add to cart
Get Quote
1 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 469.00 USD Add to cart
Get Quote
1 Tubing, 750 mm, 25 mm TC, i.d. 14 mm 18102728 393.00 USD Add to cart
Get Quote
1 Tubing, 1800 mm, 25 mm TC, i.d. 14 mm 18102729 495.00 USD Add to cart
Get Quote
1 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 476.00 USD Add to cart
Get Quote
1 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 530.00 USD Add to cart
Get Quote
1 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 534.00 USD Add to cart
Get Quote
1 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 716.00 USD Add to cart
Get Quote
1 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 942.00 USD Add to cart
Get Quote
4 Gasket, 25 mm, i.d. 10 mm 18101240 92.63 USD Add to cart
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4 TC-Gasket 25 mm i.d. 10 mm 18103579 31.97 USD Add to cart
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5 Gasket 51 mm 28966886 74.16 USD Add to cart
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5 Gasket, 51 mm, i.d. 22 mm 44551203 83.32 USD Add to cart
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6 Gaskets i.d. 35 mm 44051501 79.53 USD Add to cart
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7 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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7 Clamps 25 mm 44056801 155.35 USD Add to cart
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8 Clamp, 50 mm TC, SS 44713401 10.68 USD Add to cart
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9 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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9 Valve 2-way, 3-port, i.d. 22 mm, 51 mm TC 44158301 2 374.00 USD Add to cart
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10 Blind Flange, 51 mm TC 44713501 101.82 USD Add to cart
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11 Pressure sensor, i.d. 10mm, 25mm TC 28934323 4 554.00 USD Add to cart
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12 Safety valve, 3 bar, 51 mm TC 18573801 3 090.00 USD Add to cart
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13 T-Junction, i.d. 10 mm 18100363 498.00 USD Add to cart
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14 Castors assembly kit 18117151 3 928.00 USD Add to cart
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15 Nozzle Piping, Chromaflow 400, i.d. 1/2", 51 mm TC 18117201 3 055.00 USD Add to cart
Get Quote

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Column leakage

Strange peaks

Poor reproducibility

Unusual column appearance

Poor product recovery

Drifting baseline

Contact