FAQ
Why is there arcing during the ExcelGel run?
Arcing along the sides of the gel can be due to excessive condensation on the cooling plate. Condensed water can collect along the edges of the gel, drawing out buffer from the gel itself. Current run along the edge of the gel (since the gel itself gives some resistance) and this current on the edge could lead to arcing.
Alternatively, water was used to underlay the GelBond backing onto the cooling plate, and excess water was squeezed out along the edges of the gel leading to the same situation. Use mineral oil instead of water.
What causes the dye front to curve up (smiling) when Multiphor II is used for the second-dimension SDS-PAGE?
Excessive heat causes smiling of gels. Ensure that the thermostatic circulator is connected to the Multiphor II unit and functioning correctly.
Low current in ExcelGel at start of the 2nd dimension run. Why?
Poor or no contact between the electrodes and electrode strips. Make sure that the electrode strips are correctly placed. If a small gel or part of a gel is used, place it in the middle, so even pressure is applied to the gel across the entire length.