FAQ

Accessories

Connecting the column to a system

Figure. Packing the column, parts identification.

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# Product Name Product Code Price
2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 347.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 371.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 313.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 426.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 433.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 482.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 485.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 651.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 856.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 12 mm 18020000 28.04 USD Add to cart
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3 Gasket 25 mm, i.d. 12 mm 44550120 349.00 USD Add to cart
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4 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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4 Clamps 25 mm 44056801 155.35 USD Add to cart
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5 Blind Flange, including gasket, 25 mm 18100125 69.96 USD Add to cart
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6 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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7 Hydraulic Inlet Valve (Ball Valve) for FineLINE 70–350 Columns 18110537 469.00 USD Add to cart
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8 Locking bar 11003417 11 900.00 USD Add to cart
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9 Manometer Kit, 0-10 bar 18103107 2 830.00 USD Add to cart
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10 Pressure relief valve i.d. 10 mm, 25 mm TC 18110697 1 617.00 USD Add to cart
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11 Safety Valve, 8 bar 18103580 3 012.00 USD Add to cart
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12 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 429.00 USD Add to cart
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13 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 454.00 USD Add to cart
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13 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 282.00 USD Add to cart
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13 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 544.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 1 946.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 141.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 238.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 2 919.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 3 697.00 USD Add to cart
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14 Gasket 51 mm 28966886 74.16 USD Add to cart
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14 Gasket 51 mm, i.d. 22 mm 44551102 403.00 USD Add to cart
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15 Clamp, 50 mm TC, SS 44713401 9.71 USD Add to cart
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Connecting the column to a system

Figure. Running the column, parts identification.

 

 

 

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# Product Name Product Code Price
2 Tubing, 900 mm, 25 mm TC, i.d.10 mm 18101262 331.00 USD Add to cart
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2 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 347.00 USD Add to cart
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2 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 371.00 USD Add to cart
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2 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 313.00 USD Add to cart
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2 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 426.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 433.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 482.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 485.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 651.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 4 m, i.d. 9.5 mm, 25 mm TC 28405378 856.00 USD Add to cart
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3 Gasket, 25 mm, i.d. 12 mm 18020000 28.04 USD Add to cart
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3 Gasket 25 mm, i.d. 12 mm 44550120 349.00 USD Add to cart
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4 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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4 Clamps 25 mm 44056801 155.35 USD Add to cart
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5 Blind Flange, including gasket, 25 mm 18100125 69.96 USD Add to cart
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6 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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7 Valve, 4-port, 4-way, i.d. 10 mm 18101257 1 965.00 USD Add to cart
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8 Locking bar 11003417 11 900.00 USD Add to cart
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9 Manometer Kit, 0-10 bar 18103107 2 830.00 USD Add to cart
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11 Safety Valve, 8 bar 18103580 3 012.00 USD Add to cart
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12 T-Junction, 2 × 25 mm TC, i.d. 10 mm; 1 × 50 mm TC, i.d. 22 mm 28405775 3 429.00 USD Add to cart
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13 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 454.00 USD Add to cart
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13 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 282.00 USD Add to cart
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13 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 544.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 1 946.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 141.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 238.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 2 919.00 USD Add to cart
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13 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 3 697.00 USD Add to cart
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14 Gasket 51 mm 28966886 74.16 USD Add to cart
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14 Gasket 51 mm, i.d. 22 mm 44551102 403.00 USD Add to cart
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15 Clamp, 50 mm TC, SS 44713401 9.71 USD Add to cart
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16 Top Valve for Air Traps 18112144 1 373.00 USD Add to cart
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18 Filter 215 nm 11000733 676.00 USD Add to cart
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18 Filter 260 nm 11000734 645.00 USD Add to cart
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18 Filter 280 nm 11000735 519.00 USD Add to cart
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18 Filter 405 nm 11000736 617.00 USD Add to cart
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18 Empty Filter Holder 11000738 202.00 USD Add to cart
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18 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 8 932.00 USD Add to cart
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18 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
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18 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
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18 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
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18 O-ring kit 28969705 133.83 USD Add to cart
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19 Flow cell i.d. 10 mm, 25 mm TC 18100065 3 378.00 USD Add to cart
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19 Filter Kit 280 nm 19243301 983.00 USD Add to cart
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# Product Name Product Code Price
1 Tubing with Sanitary Fittings, 50 cm, i.d. 9.5 mm, 25 mm TC 28405373 433.00 USD Add to cart
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2 Tubing with Sanitary Fittings, 80 cm, i.d. 9.5 mm, 25 mm TC 28405375 482.00 USD Add to cart
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3 Tubing with Sanitary Fittings, 1 m, i.d. 9.5 mm, 25 mm TC 28405376 485.00 USD Add to cart
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4 Tubing with Sanitary Fittings, 2 m, i.d. 9.5 mm, 25 mm TC 28405377 651.00 USD Add to cart
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5 Tubing, 300 mm, 25 mm TC, i.d. 10 mm 18101285 313.00 USD Add to cart
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6 Tubing, 1400 mm, 25 mm TC, i.d.10 mm 18101263 347.00 USD Add to cart
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7 Tubing, 1700 mm, 25 mm TC, i.d. 10 mm 18101264 371.00 USD Add to cart
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8 Tubing, 2000 mm, 25 mm TC, i.d. 10 mm 18101287 426.00 USD Add to cart
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9 Gasket, 25 mm, i.d. 12 mm 18020000 28.04 USD Add to cart
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10 Gasket 25 mm, i.d. 12 mm 44550120 349.00 USD Add to cart
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11 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
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12 Clamps 25 mm 44056801 155.35 USD Add to cart
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13 Valve 4-port, 2-way ID 10 18101256 1 726.00 USD Add to cart
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Spare parts

O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. We recommend you to keep a set of each on your site, for code numbers refer to the table below.

Ethylene propylene diene change characteristics when exposed to some organic chemicals. For repetitive long term use, use Perfluor rubber (PFR) polymers.

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# Product Name Product Code Price
1 O-ring 350 x 5 mm 18115372 697.00 USD Add to cart
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2 O-ring 15.2 x 3.5 mm 18115374 113.29 USD Add to cart
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3 O-ring 85.35 x 3.53 mm 18115373 161.95 USD Add to cart
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4 O-ring 329.5 x 6.99 mm 18115375 652.00 USD Add to cart
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5 O-ring 350 x 5 mm 18115376 1 441.00 USD Add to cart
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6 O-ring 15.2 x 3.5 mm 18115378 318.00 USD Add to cart
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7 O-ring 85.35 x 3.53 18115377 272.00 USD Add to cart
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8 O-ring 329.5 x 6.99 mm 18115379 2 531.00 USD Add to cart
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9 O-Ring Kit, FineLINE 350, PFR 18113698 5 729.00 USD Add to cart
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# Product Name Product Code Price
5 Net, adaptor 2 µm 10 mm in/outlet 11003408 8 417.00 USD Add to cart
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5 Net, adaptor 10 µm, 10 mm in/outlet 11003410 8 400.00 USD Add to cart
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5 Net, adaptor 2 µm, 15 mm in/outlet 11003412 8 453.00 USD Add to cart
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5 Net adaptor, complete 10 µm, 15 mm in / outlet 11003413 8 147.00 USD Add to cart
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6 Net, end-piece complete 2 µm, 15 mm in/outlet 11003409 8 109.00 USD Add to cart
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6 Net, end-piece complete 10 µm, 15 mm in /outlet 11003411 8 093.00 USD Add to cart
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7 O-ring 350 x 5 mm 18115372 697.00 USD Add to cart
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7 O-ring 350 x 5 mm 18115376 1 441.00 USD Add to cart
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8 O-ring 15.2 x 3.5 mm 18115374 113.29 USD Add to cart
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8 O-ring 15.2 x 3.5 mm 18115378 318.00 USD Add to cart
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9 O-ring 85.35 x 3.53 mm 18115373 161.95 USD Add to cart
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9 O-ring 85.35 x 3.53 18115377 272.00 USD Add to cart
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10 O-ring 329.5 x 6.99 mm 18115375 652.00 USD Add to cart
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10 O-ring 329.5 x 6.99 mm 18115379 2 531.00 USD Add to cart
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11 Piston seal 18114999 572.00 USD Add to cart
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12 Guide ring 18115445 278.00 USD Add to cart
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16 Screw M6 x 20 18115446 115.47 USD Add to cart
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17 Washer M6 x 12 11003414 107.31 USD Add to cart
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19 Bolt, M12 x 25 18101265 112.05 USD Add to cart
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20 Washer 13 x 24 x 2.5 18101261 116.70 USD Add to cart
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21 Locking bar 11003417 11 900.00 USD Add to cart
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23 Bolt M24 Spec. 350 mm* 11003422 1 208.00 USD Add to cart
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23 Bolt M24 Spec. 495 mm 11003423 1 532.00 USD Add to cart
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24 O-ring 6.07 x 1.78 mm 18115448 125.44 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact