FAQ

Spare parts

Figure. FineLINE 70/70L.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
5 Net, adaptor complete 2 µm 18115361 1 797.00 USD Add to cart
Get Quote
5 Net, adaptor complete 10 µm 18115367 1 921.00 USD Add to cart
Get Quote
6 Net, end-piece complete 2 µm 18115362 1 857.00 USD Add to cart
Get Quote
6 Net, end-piece complete10 µm 18115368 1 947.00 USD Add to cart
Get Quote
7 O-ring 74.6 x 3.5 mm 18111419 28.49 USD Add to cart
Get Quote
7 O-ring 74.6 x 3.53 mm 18115363 215.00 USD Add to cart
Get Quote
8 O-ring 5.3 x 2.4 mm 18110392 14.16 USD Add to cart
Get Quote
8 O-ring 5.3 X 2.4 mm 18110551 69.95 USD Add to cart
Get Quote
9 O-ring 61.2 x 3 mm 18115364 32.66 USD Add to cart
Get Quote
10 O-ring 61.9 x 3.5 mm 18111418 19.87 USD Add to cart
Get Quote
10 O-ring 61.9 x 3.53 mm 18115365 328.00 USD Add to cart
Get Quote
11 Piston seal 18103956 68.84 USD Add to cart
Get Quote
12 Guide ring 18103957 61.25 USD Add to cart
Get Quote
16 Screw, M5 x 10 18110723 29.66 USD Add to cart
Get Quote
17 Washer M6 x 12 11003414 107.31 USD Add to cart
Get Quote
18 Column Stand 100 Columns 18103110 1 035.00 USD Add to cart
Get Quote
21 Locking Bar 18115366 354.00 USD Add to cart
Get Quote
22 Washer 10.5 x 30 mm 18110682 183.12 USD Add to cart
Get Quote
23 Bolt M10 Spec. 375 mm 18112223 493.00 USD Add to cart
Get Quote

O-rings should be checked regularly for wear and replaced when needed or at least annually. Worn O-rings may not seal properly. We recommend you to keep a set of each on your site or a O-ring kit (O-ring kit available in PFR), for code numbers refer to the table below.

The column is delivered with O-rings in Ethylene propylene diene (EPDM). Ethylene propylene diene change characteristics when exposed to some organic chemicals. For repetitive long term use, use Perfluor rubber (PFR) polymers.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 O-ring 74.6 x 3.5 mm 18111419 28.49 USD Add to cart
Get Quote
2 O-ring 74.6 x 3.53 mm 18115363 215.00 USD Add to cart
Get Quote
3 O-ring 5.3 x 2.4 mm 18110392 14.16 USD Add to cart
Get Quote
4 O-ring 5.3 X 2.4 mm 18110551 69.95 USD Add to cart
Get Quote
5 O-ring 61.9 x 3.5 mm 18111418 19.87 USD Add to cart
Get Quote
6 O-ring 61.9 x 3.53 mm 18115365 328.00 USD Add to cart
Get Quote
7 O-RING KIT FINELINE 70/70L 18115543 634.00 USD Add to cart
Get Quote
8 Piston seal 18103956 68.84 USD Add to cart
Get Quote

Accessories

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Tubing, 1200 mm, i.d. 2.9 mm, o.d. 3/16", S8 18116975 202.00 USD Add to cart
Get Quote
2 Tubing, 400 mm, i.d. 2.9 mm, o.d. 3/16", S6 18116970 171.87 USD Add to cart
Get Quote
3 Connector, 25 mm TC - UNF 5/16" Female 18116922 160.67 USD Add to cart
Get Quote
4 TC Gasket, 25 mm, i.d. 4 mm 18116924 251.00 USD Add to cart
Get Quote

Connecting the column with ÄKTApilot

Figure. Running the column, parts identification.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Column Stand 100 Columns 18103110 1 035.00 USD Add to cart
Get Quote
2 Tubing, 300 mm, 25 mm TC, i.d. 6 mm 18000542 316.00 USD Add to cart
Get Quote
2 Tubing, 750 mm, 25 mm TC, i.d. 6 mm 18000543 338.00 USD Add to cart
Get Quote
2 Tubing, 1250 mm, 25 mm TC, i.d. 6 mm 18000544 344.00 USD Add to cart
Get Quote
2 Tubing, 1500 mm, 25 mm TC, i.d. 6 mm 18000545 355.00 USD Add to cart
Get Quote
2 Tubing, 2000 mm, 25 mm TC, i.d. 6 mm 18000547 398.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 50 cm, i.d. 6 mm, 25 mm TC 28405368 425.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 80 cm, i.d. 6 mm, 25 mm TC 28405369 446.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 2 m, i.d. 6 mm, 25 mm TC 28405371 573.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 4 m, i.d. 6 mm, 25 mm TC 28405372 695.00 USD Add to cart
Get Quote
3 Gasket 25 mm, i.d. 6 mm 18001927 15.42 USD Add to cart
Get Quote
3 Gasket, 25 mm i.d 6 mm 44551344 327.00 USD Add to cart
Get Quote
4 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
Get Quote
4 Clamps 25 mm 44056801 155.35 USD Add to cart
Get Quote
5 Blind Flange, including gasket, 25 mm 18100125 69.96 USD Add to cart
Get Quote
6 Valve 4-Port 2-Way ID 6 mm 18575701 1 694.00 USD Add to cart
Get Quote
7 Valve, 4-port, 4-way, i.d. 6 mm, 25 mm TC 18575801 1 758.00 USD Add to cart
Get Quote
8 Locking Bar 18115366 354.00 USD Add to cart
Get Quote
9 Manometer Kit, 0-10 bar 18103107 2 830.00 USD Add to cart
Get Quote
11 Safety Valve, 8 bar 18103580 3 012.00 USD Add to cart
Get Quote
12 T-Junction, 2 × 25 mm TC, i.d. 6 mm; 1 × 50 mm TC, i.d. 22 mm 28405765 831.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 454.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 282.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 544.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 1 946.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 141.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 238.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 2 919.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 3 697.00 USD Add to cart
Get Quote
14 Gasket 51 mm 28966886 74.16 USD Add to cart
Get Quote
14 Gasket 51 mm, i.d. 22 mm 44551102 403.00 USD Add to cart
Get Quote
15 Clamp, 50 mm TC, SS 44713401 9.71 USD Add to cart
Get Quote
16 Top Valve for Air Traps 18112144 1 373.00 USD Add to cart
Get Quote
18 Filter 215 nm 11000733 676.00 USD Add to cart
Get Quote
18 Filter 260 nm 11000734 645.00 USD Add to cart
Get Quote
18 Filter 280 nm 11000735 519.00 USD Add to cart
Get Quote
18 Filter 405 nm 11000736 617.00 USD Add to cart
Get Quote
18 Empty Filter Holder 11000738 202.00 USD Add to cart
Get Quote
18 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 8 932.00 USD Add to cart
Get Quote
18 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
Get Quote
18 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
Get Quote
18 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
Get Quote
18 O-ring kit 28969705 133.83 USD Add to cart
Get Quote
19 Flow cell, i.d. 6 mm, 25 mm TC 18100066 3 206.00 USD Add to cart
Get Quote
19 Filter Kit 280 nm 19243301 983.00 USD Add to cart
Get Quote

Connecting the column with ÄKTApilot

Figure. Packing the column, parts identification.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Column Stand 100 Columns 18103110 1 035.00 USD Add to cart
Get Quote
2 Tubing, 300 mm, 25 mm TC, i.d. 6 mm 18000542 316.00 USD Add to cart
Get Quote
2 Tubing, 750 mm, 25 mm TC, i.d. 6 mm 18000543 338.00 USD Add to cart
Get Quote
2 Tubing, 1250 mm, 25 mm TC, i.d. 6 mm 18000544 344.00 USD Add to cart
Get Quote
2 Tubing, 1500 mm, 25 mm TC, i.d. 6 mm 18000545 355.00 USD Add to cart
Get Quote
2 Tubing, 2000 mm, 25 mm TC, i.d. 6 mm 18000547 398.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 50 cm, i.d. 6 mm, 25 mm TC 28405368 425.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 80 cm, i.d. 6 mm, 25 mm TC 28405369 446.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 1 m, i.d. 6 mm, 25 mm TC 28405370 450.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 2 m, i.d. 6 mm, 25 mm TC 28405371 573.00 USD Add to cart
Get Quote
2 Tubing with Sanitary Fittings, 4 m, i.d. 6 mm, 25 mm TC 28405372 695.00 USD Add to cart
Get Quote
3 Gasket 25 mm, i.d. 6 mm 18001927 15.42 USD Add to cart
Get Quote
3 Gasket, 25 mm i.d 6 mm 44551344 327.00 USD Add to cart
Get Quote
4 TC Clamp 25mm SS 18100131 38.30 USD Add to cart
Get Quote
4 Clamps 25 mm 44056801 155.35 USD Add to cart
Get Quote
5 Blind Flange, including gasket, 25 mm 18100125 69.96 USD Add to cart
Get Quote
6 Valve 4-Port 2-Way ID 6 mm 18575701 1 694.00 USD Add to cart
Get Quote
7 Hydraulic Inlet Valve (Ball Valve) for FineLINE 70–350 Columns 18110537 469.00 USD Add to cart
Get Quote
8 Locking Bar 18115366 354.00 USD Add to cart
Get Quote
9 Manometer Kit, 0-10 bar 18103107 2 830.00 USD Add to cart
Get Quote
10 Pressure Relief Valve for FineLINE 70,100, and 200 Columns 18110536 1 666.00 USD Add to cart
Get Quote
11 Safety Valve, 8 bar 18103580 3 012.00 USD Add to cart
Get Quote
12 T-Junction, 2 × 25 mm TC, i.d. 6 mm; 1 × 50 mm TC, i.d. 22 mm 28405765 831.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=90 cm, i.d.19 mm, 51 mm TC 28404230 454.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=200 cm, i.d.19 mm, 51 mm TC 28404232 3 322.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 400 cm, i.d.19 mm, 51 mm TC 28404233 1 282.00 USD Add to cart
Get Quote
13 Tubing with sanitary fittings, L=140 cm, i.d.19 mm, 51 mm TC 28404235 544.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 50 cm, i.d. 19.1 mm, 50 mm TC 28405379 1 946.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 80 cm, i.d. 19.1 mm, 50 mm TC 28405380 2 141.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 1 m, i.d. 19.1 mm, 50 mm TC 28405381 2 238.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 2 m, i.d. 19.1 mm, 50 mm TC 28405382 2 919.00 USD Add to cart
Get Quote
13 Tubing with Sanitary Fittings, 4 m, i.d. 19.1 mm, 50 mm TC 28405383 3 697.00 USD Add to cart
Get Quote
14 Gasket 51 mm 28966886 74.16 USD Add to cart
Get Quote
14 Gasket 51 mm, i.d. 22 mm 44551102 403.00 USD Add to cart
Get Quote
15 Clamp, 50 mm TC, SS 44713401 9.71 USD Add to cart
Get Quote

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

Contact