Accessories
Front view
Part | Description |
---|---|
1 | Fractionation indicator Symbol indicating that fractionation is ongoing. Do not open the door while the indicator is lit. |
2 | Door |
3 | Window |
4 | Door handle |
5 | Tubing connector for outlet valve tubing |
6 | Vents |
Rear view
Part | Description |
---|---|
1 | Vents |
2 | UniNet-9 D-type connector (for communication and power supply) |
3 | Waste tube |
Interior
Part | Description |
---|---|
1 | Fractionation arm guide rail |
2 | Fractionation arm main rail |
3 | Lamp |
4 | Tubing guide |
5 | Tubing connection |
6 | Dispenser head |
7 | Fractionation arm |
8 | Tubing guide |
9 | Height exclusion bar |
10 | Waste funnel |
11 | Waste tube |
12 | Tray catch |
13 | Waste groove, in case of overflow |
14 | Tray guides |
Dispenser head
Part | Description |
---|---|
1 | Dispenser head |
2 | Nozzle |
3 | Dispenser head cover |
4 | Accumulator (back part of Dispenser head) |
5 | Drop sync sensor |
6 | Type code reader |
# | Product Name | Product Code | Price | |
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UNIPLATE Collection and Analysis Microplate, 24-well, 10 ml, natural polypropylene, round well bottom | 28420867 | 343.00 USD |
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UNIPLATE Collection and Analysis Microplate, 96-well, 2 ml, natural polypropylene, round well bottom | 28420869 | 273.00 USD |
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UNIPLATE Collection and Analysis Microplate, 48-well, 5 ml, natural polypropylene, rectangular well, flat bottom | 28420874 | 350.00 USD |
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Cassette tray | 28954209 | 310.00 USD |
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Cassette for fraction collection in deep-well plate | 28954212 | 194.58 USD |
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Cassette for 50 ml tubes | 28956402 | 197.68 USD |
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Cassette, for 15 ml tubes | 28956404 | 197.68 USD |
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Cassette for fraction collection in 8 mL tubes | 28956425 | 198.72 USD |
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Cassette, for 3 ml tubes | 28956427 | 198.72 USD |
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Rack, for 50 ml tubes | 28980319 | 774.00 USD |
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Rack for fraction collection in 250 mL bottles | 28981873 | 1,660.00 USD |
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Fraction Collector F9-C | 29027743 | 12,500.00 USD |
Add to cart
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Cable 2.5m UniNet-9 D-type | 29032425 | 140.76 USD |
Add to cart
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Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Fraction collector F9-C
Issues related to Fraction collector F9-C
Possible cause | Suggested remedy |
---|---|
The cable between the fraction collector and the ÄKTA pure instrument is not connected |
Generate a System error report and contact Service. |
A fuse in the instrument ICU is broken |
The ICU needs to be changed. Generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Obstruction inside the fraction collector |
Switch off the instrument and check for obstruction inside the fraction collector. Try to move the fractionation arm by hand. Switch on the instrument. If this error is recurrent, generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Salt crystals or protein residuals block the accumulator |
Restart the instrument and perform an accumulator wash. |
Mechanical error |
If this error is recurrent, generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Too many commands are pending in the fraction collector |
The reason could be that too many fraction collector instructions have been sent. Wait for a while and try again. |
Possible cause | Suggested remedy |
---|---|
Cassette tray or tube rack is inserted in the wrong direction |
Take it out and insert it in the correct direction. |
Unclean Cassette type code reader |
Clean the dispenser head and its four diode windows using a cloth and a mild cleaning agent or 20% ethanol. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. |
Unclean Cassette type codes |
Clean the Cassette type codes. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. If this error is recurrent, set the Fraction collector configuration manually in UNICORN. In System Control, select System:Settings. Navigate to Fraction collector:Cassette configuration and select Manual. |
Possible cause | Suggested remedy |
---|---|
The Tray guides in the fractionation chamber are missing |
Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. |
The Tray catch is broken |
Contact service. |
Possible cause | Suggested remedy |
---|---|
Unclean Drop sync sensor diode windows |
Clean the Drop sync sensor diode windows. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for location of the Drop sync sensor diode windows and cleaning instructions. If this error is recurrent, generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Liquid spatter in the fraction collector |
Change to another type of tubes or deepwell plates or collect fraction volumes of maximum two thirds of the volume of the tubes. Clean the diode windows of the drop sync sensor more frequently than once a week, using a cloth and a mild cleaning agent or 20% ethanol. |
Possible cause | Suggested remedy |
---|---|
Air in the flow path |
If this error is recurrent, generate a System error report and contact Service. Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook. |
Unclean Drop sync sensor diode windows |
Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook for more information. If this error is recurrent, generate a System error report and contact Service. |
Too high flow rate |
Decrease the flow rate. |
Possible cause | Suggested remedy |
---|---|
Air in the flow path |
Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service. |
Unclean diode windows in the Drop sync sensor |
Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Too high flow rate |
Decrease the flow rate. |
Air in the flow path |
Check the flow path for air. Fill system and purge pumps according to Section 6.4.1 System pumps in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service. |
Unclean diode windows in the Drop sync sensor |
Clean the diode windows of the Drop sync sensor. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If this error is recurrent, generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
Wrong tube dimensions are used |
Check that the used tubes have the right dimensions. See Fraction collector tubes, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook. |
QuickRelease function is worn out |
Order a new Cassette. See Chapter 11 in the ÄKTA pure System Handbook for ordering information. |
Possible cause | Suggested remedy |
---|---|
Unsupported deep well plate model |
Check that the deep well plates are supported. See Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook. |
Possible cause | Suggested remedy |
---|---|
The Cassette is turned in the wrong direction |
See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook for information of how to place the Cassettes. |
Objects or dirt under the Cassette |
Remove the object or dirt. |
Possible cause | Suggested remedy |
---|---|
The Tray guides in the fractionation chamber are missing |
Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. |
The Cassette tray is facing the wrong direction |
Make sure that the front of the tray (marked with the GE-logotype) is facing outwards. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook. |
Dirt under the Cassette tray |
Remove the dirt. |
Possible cause | Suggested remedy |
---|---|
The Cassette tray is facing the wrong direction |
Check that the GE-logotype is facing outwards when the tray is inserted into the fraction collector. |
Some of the tubes or plates are incorrectly placed in the Cassettes |
Check that all tubes and plates are correctly inserted in the Cassettes. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook. |
Some of the tubes or plates have the wrong dimensions |
Check that the deep well plates and the tubes used are of the right type. See Fraction collector tubes and Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook for information about supported tubes and plates. |
Possible cause | Suggested remedy |
---|---|
The Tray guides in the fractionation chamber are missing |
Replace the Tray guides. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. |
The Cassette tray is facing the wrong direction |
Check that the GE-logotype is facing outwards. |
The Cassette type codes are unclean |
Clean the the Cassette type codes. |
The QuickRelease of a Cassette is in open position |
Close the Cassette. See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook. |
The Cassette code reader diode window is unclean |
Clean the Cassette code reader diode window. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. |
The automatic scanning is turned off in UNICORN |
Make sure that the automatic scanning is turned on in UNICORN software. In System Control, select System:Settings. Navigate to Fraction collector:Cassette configuration and select Automatic. |
Wells in deep well plates are prefilled to a volume above 25% of the total well volume |
Full scan will not work with prefilled wells during these conditions. |
Hardware error |
Generate a System error report and contact Service. |
Possible cause | Suggested remedy |
---|---|
The light has been turned off in UNICORN |
Turn on the light in UNICORN. In System Control, select System: Settings. Navigate to Fraction collector:Fraction collector lamp and select Lamps On. |
The lamp is broken |
Contact Service. |
Possible cause | Suggested remedy |
---|---|
The waste tubing is positioned so that the flow is obstructed |
Untwist the waste tubing. |
The waste container is placed in a position higher than the waste outlet |
Place the waste container in a position lower than the waste outlet. |
The waste tubing is blocked |
Clean or replace the waste tubing. |
Possible cause | Suggested remedy |
---|---|
Check the position of the waste funnel |
Refit the waste funnel. |
Possible cause | Suggested remedy |
---|---|
Salt residuals in the tubing |
Perform a Fraction collector wash, see Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. If persistent, contact Service. |
Possible cause | Suggested remedy |
---|---|
Leakage on the wet side of the instrument |
Localize the leakage and take care of the leakage, for example by tightening connectors. |
Air in pumps |
Purge pumps. See Section 6.4.1 System pumps in the ÄKTA pure System Handbook. |
Bad pump function |
See troubleshooting of pumps in Section 9.5 in the ÄKTA pure System Handbook. |
Leakage inside the Frac chamber |
Contact Service. |
Possible cause | Suggested remedy |
---|---|
The fingertight connector on the Dispenser head is not tight enough |
Tighten the connector. Replace the connector if the leakage is persistent. |
The waste tubing or the waste funnel is blocked. |
Make sure that there is no blockage or clogging and that the waste tubing not is bent. |
Leakage in the Frac arm |
Contact service. |
Possible cause | Suggested remedy |
---|---|
Unclean diode windows in the Drop sync sensor or unclean nozzle |
Clean the Drop sync diode windows and the nozzle. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. Use the Fraction collector cleaning position. |
Too high flow rate during usage of Drop sync |
Use a flow rate below 2 ml/min. |
One or more Cassettes have empty positions |
Make sure that all Cassette positions contain tubes or plates. |
The tubes are flooded |
Make sure that the fraction volume is adapted to the tube volume. Fraction tubes have not been replaced by empty tubes when the Fraction collector was opened. |
Possible cause | Suggested remedy |
---|---|
The door of the fraction collector is not properly closed |
Close the door. |
Possible cause | Suggested remedy |
---|---|
Quick scan has not detected the correct Cassette |
Clean the Cassette type code of the Cassette. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. |
Unsupported deep well plate is used. |
Make sure that approved deep well plates are used. See Deep well plates, section 3.2.3 Cassettes, Cassette tray and racks, in the ÄKTA pure System Handbook. |
Wells in deep well plates are prefilled to a volume above 25% of the total well volume |
Full scan will not work with prefilled wells during these conditions. |
The deep well plate is not correctly positioned in the Cassette |
See Prepare and insert the Cassette tray, section 6.7.1 Fraction collector F9-C, in the ÄKTA pure System Handbook for information of how to place the deep well plate. |
Dirt on the nozzle or Drop sync sensor diode windows. Dirt may effect where the drops fall. |
Clean nozzle and Drop sync sensor diode windows. See Section 8.6.4 Clean Fraction collector F9-C in the ÄKTA pure System Handbook. |
Possible cause | Suggested remedy |
---|---|
Some parts in a method require that the door of the fraction collector is closed |
Do not open the door when the Fractionation indicator is lit. |
Possible cause | Suggested remedy |
---|---|
The Detector - Outlet valve delay volume has been set to zero. |
|
Possible cause | Suggested remedy |
---|---|
Action: End a manual run before starting a method run. |
Possible cause | Suggested remedy |
---|---|
Action:
- the tubing between the outlet valve and the fraction collector - the internal tubing of the fraction collector. Note: |
Possible cause | Suggested remedy |
---|---|
Action: If possible, place the Cassettes close to waste, i.e., position 5 or 6 during fractionation. |
Possible cause | Suggested remedy |
---|---|
The door of the fraction collector has been opened between two runs. When the instrument is in state Ready and the door is opened and closed, the Fraction collector content is reset. |
If fractionation will continue after the last fraction of the first run, do not open the door of the fraction collector between two runs. |
Possible cause | Suggested remedy |
---|---|
The combination of high flow rates and liquids with low surface tension might lead to spillage in the Fraction collector. |
If possible use Cassette positions 5 and 6. Lower the flow rate when using liquids with low surface tension. |