FAQ
The main reason for the recommended differences in coupling time is the slower mass transfer in the larger particles in the FF-media (90 µm) compared with HP media (34 µm). However, 15-30 min is often enough to give efficient coupling, also in the FF-media. The coupling time really has to be optimized for each protein, the instructions are more to be seen as general guidelines, but they will mostly give a sufficiently good coupling.
My ligand has low solubility and I wonder if I can use urea or guanidine HCl in the coupling buffer?
Urea and guanidine HCl cannot be used in the coupling buffer since both of these contain primary amines that would rapidly couple to the preactivated medium by the same mechanism as the ligand.
Accessories
# | Product Name | Product Code | Price | |
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1 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 99.55 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" | 18111253 | 96.26 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" | 18111368 | 73.48 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16" | 18111583 | 85.34 USD |
Add to cart
|
1 | Tubing i.d. 0.25 mm, o.d. 1/16" | 18112095 | 66.24 USD |
Add to cart
|
2 | Fingertight connector 1/16" male, narrow | 28401081 | 108.65 USD |
Add to cart
|
# | Product Name | Product Code | Price | |
---|---|---|---|---|
1 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 99.55 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" | 18111253 | 96.26 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" | 18111368 | 73.48 USD |
Add to cart
|
1 | PEEK Tubing, 2 m, i.d. 1.0 mm, o.d. 1/16" | 18111583 | 85.34 USD |
Add to cart
|
1 | Tubing i.d. 0.25 mm, o.d. 1/16" | 18112095 | 66.24 USD |
Add to cart
|
2 | Fingertight connector 1/16" male, narrow | 28401081 | 108.65 USD |
Add to cart
|
3 | Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 123.16 USD |
Add to cart
|
# | Product Name | Product Code | Price | |
---|---|---|---|---|
1 | Connector 1/16" Male/Luer Female | 18111251 | 109.24 USD |
Add to cart
|
Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
General advice to achieve good performance
Before using the column make sure that:
- Correct system has been selected in UNICORN System Control
- Correct wavelength has been set for UV/UPC monitor
- All tubing has been properly connected and tubing is not longer than needed
- All connectors are free from leakage, verified by passing a leakage test
- No tubing is folded or twisted
- Online filter, if used, is changed on a regular basis
- Correct buffers are used for the chosen columns and proteins
- All inlet tubing has been immersed in correct buffer solutions
- Enough buffer has been prepared
- Buffers have been equilibrated to the environment temperature
- Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
- Suitable columns have been selected for the target proteins
- Column meets pressure requirements for selected medium
- Columns have been cleaned and prepared according to column instructions
- Samples have been clarified by centrifugation and/or filtration prior to sample loading
- Samples have been adjusted to binding buffer conditions
- Auto sampler (if used) has been prepared according to user manual
- The fraction collector has been filled with appropriate number of microtiter plates or tubes
- Appropriate arrangement for waste handling has been prepared