What is more sensitive, film or CCD? I have a feeling that film is more sensitive.
Yes, film can be more sensitive, but only very little. In a 2-fold dilution series of transferring, it can be one extra band. Usually this is not so important, more important is the decreased dynamic range with CCD camera. With CCD camera you have easy control and can easily avoid saturation of your signals, thereby get quantifiable data. Film can be more sensitive if you expose for longer time. But long exposure time also results in higher background which can lower the sensitivity.