FAQ

Spare parts

O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets. We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for code numbers refer to the table below.

23 µm nets are supplied with the column and recommended for beads with an average particle diameter between > 70 µm. For an average particle diameter < 70 µm use 10µm nets.

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# Product Name Product Code Price
1 O-Ring End Piece 202.8X3.5 EPDM 18848901 60.62 USD Add to cart
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2 O-ring 7.3 x 2.4 mm 18110390 15.60 USD Add to cart
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3 O-ring 184.1 x 8.4 mm 18110405 73.58 USD Add to cart
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4 Support net, adaptor 200 column 18025256 54.24 USD Add to cart
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5 Support net, end-piece 200 column 18025255 61.80 USD Add to cart
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6 Net, 10 μm adaptor 200 column PA 18025276 308.00 USD Add to cart
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7 Net, 10 μm, end-piece 200 column PA 18025277 296.00 USD Add to cart
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8 Net, 23 μm adaptor 200 column PP 18925301 315.00 USD Add to cart
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9 Net, 23 μm, end-piece 200 column PP 18925401 315.00 USD Add to cart
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10 Sealing ring 18110409 23.95 USD Add to cart
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IMPORTANT: The following spare parts should be ordered for columns with serial numbers lower than the following serial numbers.

100 032 50    (INdEX 100/500)

100 013 95    (INdEX 100/950)

200 027 50    (INdEX 200/500)

200 019 95    (INdEX 200/950)

Pos. Designation INdEX 100 INdEX 200 Qty
5 End-piece 18-1103-81 18-1104-01 1
7 Adaptor - 18-1104-00 1
8 TC-connector, bottom - -  
9 TC-connector 18-1103-85 18-1104-06 2
18 O-ring 5.1x1.6 EPDM 18-1107-67 - 5
18 O-ring 7.1x1.6 EPDM - 18-1107-72  

 

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# Product Name Product Code Price
1 Lid, INdEX 200 column 18110402 382.00 USD Add to cart
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2 Upper flange 18110403 437.00 USD Add to cart
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3 Column tube 500 18110451 2 983.00 USD Add to cart
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3 Column tube 950 18110452 5 581.00 USD Add to cart
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4 Bottom flange 18110404 653.00 USD Add to cart
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5 End-piece 18111359 444.00 USD Add to cart
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6 Rod 500 18110407 82.17 USD Add to cart
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6 Rod 950 18110408 122.50 USD Add to cart
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7 Adaptor 18111360 1 173.00 USD Add to cart
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8 TC-connector, bottom 18111361 251.00 USD Add to cart
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9 TC-connector 18111362 122.50 USD Add to cart
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10 Support net, adaptor 200 column 18025256 54.24 USD Add to cart
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11 Net, 10 μm adaptor 200 column PA 18025276 308.00 USD Add to cart
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11 Net, 23 μm adaptor 200 column PP 18925301 315.00 USD Add to cart
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12 Support net, end-piece 200 column 18025255 61.80 USD Add to cart
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13 Net, 10 μm, end-piece 200 column PA 18025277 296.00 USD Add to cart
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13 Net, 23 μm, end-piece 200 column PP 18925401 315.00 USD Add to cart
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14 Sealing ring 18110409 23.95 USD Add to cart
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15 Valve 1/4 " 18110393 221.00 USD Add to cart
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16 O-ring 13.1 x 1.6 mm 19353701 22.04 USD Add to cart
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17 O-ring 7.3 x 2.4 mm 18110390 15.60 USD Add to cart
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18 O-ring 7.3 x 2.4 mm 18110390 15.60 USD Add to cart
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19 O-ring 184.1 x 8.4 mm 18110405 73.58 USD Add to cart
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20 O-Ring End Piece 202.8X3.5 EPDM 18848901 60.62 USD Add to cart
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21 O-ring 199.3 x 5.7 mm 18110710 32.53 USD Add to cart
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22 Washer 8.4 x 25 x 2 mm 18110398 31.36 USD Add to cart
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24 Nut M8 18110542 25.39 USD Add to cart
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25 Grip-nut 18110399 71.71 USD Add to cart
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26 Hose connector 1/4 " 18110394 55.66 USD Add to cart
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27 Adjustable Foot 18112693 43.51 USD Add to cart
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28 Stand for INdEX 140 and INdEX 200 Columns 18110361 964.00 USD Add to cart
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31 Screw 18111358 46.30 USD Add to cart
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31 Plug for the adaptor shaft 18112083 26.88 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

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