FAQ

Spare parts

O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets. We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for code numbers refer to the table below.

23 µm nets are supplied with the column and recommended for beads with an average particle diameter between > 70 µm. For an average particle diameter < 70 µm use 10µm nets.

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# Product Name Product Code Price
1 O-ring 74.6 x 3.5 mm 18111419 28.49 USD Add to cart
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2 O-ring 5.3 x 2.4 mm 18110392 14.16 USD Add to cart
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3 O-ring 61.9 x 3.5 mm 18111418 19.87 USD Add to cart
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4 Support net, adaptor 18111424 39.19 USD Add to cart
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5 Support net, end-piece 18111425 39.03 USD Add to cart
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6 Net 10µm, adaptor* 18111428 253.00 USD Add to cart
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7 Net 10µm, end-piece* 18111429 241.00 USD Add to cart
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8 Net 23µm, adaptor* 18111426 216.00 USD Add to cart
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9 Net 23µm, end-piece* 18111427 209.00 USD Add to cart
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IMPORTANT: The design of the adaptor (pos.7) for INdEX 70 and INdEX 100 has been modified, why the following spare parts should be ordered for columns with serial numbers lower than the following numbers.

Serial No.        Column

070 028 50       (INdEX 70/500)

070 011 95       (INdEX 70/950)

100 117 50       (INdEX 100/500)

100 038 95       (INdEX 100/950)

Pos. Designation INdEX 70 INdEX 100 Qty
1 Lid 18-1114-60 18-1103-82 1
14 Sealing ring 18-1103-88 18-1103-88 1
An order of the adaptor (pos.7) code no. 18-1124-01 and 18-1124-03 must be completed with a lid (pos. 1) code no. 18-1124-00 and 18-1124-02 and sealing ring (pos.14) code no. 18-1124-04.

 

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# Product Name Product Code Price
1 Lid 18112400 587.00 USD Add to cart
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2 Upper flange 18111407 203.00 USD Add to cart
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3 Column tube 500 18111414 883.00 USD Add to cart
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3 Column tube 950 18111415 1 702.00 USD Add to cart
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4 Bottom flange 18111410 262.00 USD Add to cart
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6 Rod 500 18110386 173.27 USD Add to cart
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6 Rod 950 18110387 182.24 USD Add to cart
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7 Adaptor 18112401 761.00 USD Add to cart
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8 TC-connector, bottom 18111354 305.00 USD Add to cart
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9 TC-connector 18111356 127.21 USD Add to cart
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10 Support net, adaptor 18111424 39.19 USD Add to cart
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11 Net 23µm, adaptor* 18111426 216.00 USD Add to cart
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11 Net 10µm, adaptor* 18111428 253.00 USD Add to cart
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12 Support net, end-piece 18111425 39.03 USD Add to cart
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13 Net 23µm, end-piece* 18111427 209.00 USD Add to cart
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13 Net 10µm, end-piece* 18111429 241.00 USD Add to cart
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14 Sealing ring 18112404 14.97 USD Add to cart
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15 Valve 1/4 " 18110393 221.00 USD Add to cart
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16 O-ring 13.1 x 1.6 mm 19353701 22.04 USD Add to cart
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17 O-ring 7.3 x 2.4 mm 18110390 15.60 USD Add to cart
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18 O-ring 5.3 x 2.4 mm 18110392 14.16 USD Add to cart
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19 O-ring 61.9 x 3.5 mm 18111418 19.87 USD Add to cart
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20 O-ring 74.6 x 3.5 mm 18111419 28.49 USD Add to cart
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21 O-ring 74.2 x 5.7 mm 18111420 18.41 USD Add to cart
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22 Washer 6.4 x 18 x 1.6 mm 18110396 28.38 USD Add to cart
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24 Nut M6 18110541 37.36 USD Add to cart
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25 Wing-nut M6 18110397 53.77 USD Add to cart
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26 Hose connector 1/4 " 18110394 55.66 USD Add to cart
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27 Adjustable Foot 18112693 43.51 USD Add to cart
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28 Stand complete 18110360 793.00 USD Add to cart
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31 Screw 18111358 46.30 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before packing or running the column please make sure that

  • the tubing, valves, gaskets etc. have the same inner diameter as the column in and outlets and tubing is not longer than needed.
  • the bed supports have a porosity appropriate for your chromatography medium.
  • the column has been correctly assembled, verified by passing a leakage test.
  • the chromatography medium and equipment are equilibrated in the packing buffer and to ambient temperature of packing environment.
  • that the pump is free from pulsations.
  • no pressure peaks appear.
  • that you have enough slurry volume, taken in account the compression factor of the medium.
  • packing is performed at optimal flow velocity.
  • the column meets pressure requirement for selected medium and packing procedure.

After completing the packing procedure please evaluate the efficiency of the column in terms of the height equivalent to a theoretical plate (HETP), reduced plate number (h) and the peak asymmetry factor (As).


Between batches it is recommended to perform cleaning-in-place (CIP) to remove precipitated material, strongly bound substances and other contaminants from the column bed without dismantling the column. Regular CIP is needed to ensure proper product quality and expected life time of medium and equipment.

Many issues that face chromatographers are common to all types of columns, while other issues are quite specific.
Select the appropriate equipment.

Worrying peak shape

Strange peaks

Column leakage

Poor reproducibility

Back pressure increases during operation

Unusual column appearance

Poor product recovery

Drifting baseline

Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.


Leading peak

Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As).

Unsatisfactory elution

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