FAQ

Accessories

The figure shows a MidGee cross flow cartridge, the same connectors and tubing should be used for MidGee Hoop cross flow cartridge.

The use of a permeate flow control is strongly recommended during upstream clarification of target material.

A permeate valve and a peristaltic pump is required for permeate flow control. Detailed information about the valve (56-4105-88) and peristaltic pump (18-1110-91), is available below. Permeate tubing i.d. 1.6 mm is suitable for the recommended pump.

Countries using 220 V power, have to be order power cords separately for the feed pump and the pressure display unit.

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# Product Name Product Code Price
2 Stop Plug Luer Female 56410578 28.41 USD Add to cart
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3 Connector Luer Female - 3.2 mm (0.125") Barb 56410575 28.41 USD Add to cart
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3 Connector Luer Female - 2.4 mm (0.094") Barb 56410576 28.41 USD Add to cart
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4 Peristaltic Tubing, size 14, 3.1 m, i.d. 1.6 mm, silicone 56410616 130.23 USD Add to cart
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5 Connector Luer Female - 3.2 mm (0.125") Barb 56410575 28.41 USD Add to cart
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6 Peristaltic Tubing, size 16, 3.1 m, i.d. 3.1 mm, silicone 56410617 130.23 USD Add to cart
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10 Peristaltic Pump Dual Voltage 56410653 2 658.00 USD Add to cart
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11 Tubing Valve for Tubing o.d. 6.4 mm 56410588 41.44 USD Add to cart
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12 MidGee Reservoir Kit, 175 ml 56410587 851.00 USD Add to cart
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Peristaltic Pump P-1 18111091 3 332.00 USD Add to cart
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The use of a permeate flow control is strongly recommended during upstream clarification of target material. 

A permeate valve and a peristaltic pump is required for permeate flow control. Detailed information about the valve (56-4105-88) and peristaltic pump (18-1110-91), is available below. Permeate tubing i.d. 1.6 mm is suitable for the recommended pump.

The figure shows a MidGee cross flow cartridge, the same connectors and tubing should be used for MidGee Hoop cross flow cartridge.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
2 Stop Plug Luer Female 56410578 28.41 USD Add to cart
Get Quote
3 Connector Luer Female - 2.4 mm (0.094") Barb 56410576 28.41 USD Add to cart
Get Quote
4 Peristaltic Tubing, size 14, 3.1 m, i.d. 1.6 mm, silicone 56410616 130.23 USD Add to cart
Get Quote
5 Connector Luer Female - 3.2 mm (0.125") Barb 56410575 28.41 USD Add to cart
Get Quote
6 Peristaltic Tubing, size 16, 3.1 m, i.d. 3.1 mm, silicone 56410617 130.23 USD Add to cart
Get Quote
10 Tubing Valve for Tubing o.d. 6.4 mm 56410588 41.44 USD Add to cart
Get Quote
Peristaltic Pump P-1 18111091 3 332.00 USD Add to cart
Get Quote
MidGee Reservoir Kit, 175 ml 56410587 851.00 USD Add to cart
Get Quote
Peristaltic Pump Dual Voltage 56410653 2 658.00 USD Add to cart
Get Quote

The figure shows a MidGee cross flow cartridge. The same connectors and tubing should be used for MidGee Hoop cross flow cartridges.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
2 Stop Plug Luer Female 56410578 28.41 USD Add to cart
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3 Filter Tubing Kit, i.d. 1.7 mm 28916101 460.00 USD Add to cart
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4 O-ring 3 x 1 mm 11002547 99.90 USD Add to cart
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5 Connector 5/16" Female - M6 Male 18112776 60.50 USD Add to cart
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6 Union Luerlock Female/M6 Female 18102712 54.28 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General Advice for Troubleshooting when Using Cross Flow Filters

Please check the following items during troubleshooting:

Samples volume and system working volume have been used in calculations of concentration factor and diafiltration beforehand.

Process settings of Membrane Capacity, Flux, Shear (or volumetric cross flow rate) have been properly applied for both sample characteristics and membrane pore size or NMWC designation.

Filter is properly wetted before use (also make sure that there is no air lock in the filters created by condensation on microfiltration membranes as a result of incorrect autoclave settings).

Ensure CFF filters are properly wetted before starting processing. This may be tested against new cartridge “Clean Water Flux Test or against the water flux specifications in the Certificate of Quality. Microfitlration membranes are shipped dry and are rewetted with water or buffer. Hollow fiber ultrafiltration membranes are shipped in glycerol wetting preservative. These filters may require 15-20% alcohol/water solution to restore wetting or remove glycerol. Flat sheet cassette ultrafiltration devices (for example, KvickLab and KvickFlow Cassettes) are shipped in a solution of NaOH/Glycerol which must be flushed out before use.

Prior to using cassettes, make sure the single gasket is in the correct position in the holding device and that the cassette(s) within are oriented properly in the holder.

Check integrity of cassette system using water under pressure on retentate side to 1 barg to check for leaks in the seal of the cassette with the stainless steel holding device.

Check to see that cartridges or cassettes that have been previously used are checked for integrity (air diffusion or bubble point tests) and for cleanliness (normalized Clean Water Permeability before processing).

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