FAQ
The nickel ions are very strongly bound to the ligand and will remain bound to the ligand even after incubation with 1 M NaOH or 10 mM EDTA. Also after the extreme conditions of incubating with 100 mM EDTA for 24 hours, a lot of the nickel remains bound. Therefore, recharging with nickel after normal use or CIP, even after several normal runs, is not necessary.
See the table below for differences among these products.
Product | Ni Sepharose High Performance | Ni Sepharose 6 Fast Flow | Ni Sepharose excel |
---|---|---|---|
Product codes | 17-5268-01, 17-5268-02 | 17-5318-06, 17-5318-01, 17-5318-02, 17-5318-03 | 17-3712-01, 17-3712-02, 17-3712-03 |
Base matrix | 6% highly cross-linked agarose | 6% highly cross-linked agarose | 6% highly cross-linked agarose |
Average bead size | 34 µm | 90 µm | 90 µm |
Binding capacity (mg /ml) | > 40 mg | Approx. 40 mg | > 10 mg |
Application | The high protein binding capacity and the small bead size ensures high-resolution and minimal sample dilution. | The flow properties of the highly cross-linked agarose matrix make it an excellent choice for purification scale-up. | Purification of histidine-tagged proteins from samples that can cause Ni stripping from the medium, e.g. secreted proteins in liquids containing chelators. |