FAQ
See the table below for maximum sample volumes.
PD MultiTrap G-25 | PD SpinTrap G-25 | PD MiniTrap G-25 | PD MidiTrap G-25 | PD-10 Desalting Columns | |
---|---|---|---|---|---|
Volume of prepacked medium | 500 μl/well | ~600 μl/column | 2.1 ml | 2,1 ml | 8.3 ml |
Packed bed dimensions | N/A | N/A | 0.97 × 2.8 cm | 1.3 × 2.6 cm | 1.45 × 5.0 cm |
Well/column volume | 800 μl | 1000 μl | 5 ml | 8.5 ml | 13. 5 ml |
Void volume | ~ 150 μl | ~ 150 μl | ~ 0.5 ml | ~ 1.0 ml | 2.5 ml |
Maximum sample volume | 130 μl | 180 μl | 0.5 ml | 1.0 ml | 2.5 ml |
Volume of eluted sample (gravity) | - | - | 1.0 ml | 1.5 ml | 3.5 ml |
Volume of eluted sample (spin)1 | 130 μl | 180 μl | 0.5 ml | 1.0 ml | 2.5 ml |
Recovery2 | 70% to 90% | 70% to 90% | 70% to 95% | 70% to 95% | 70% to > 95% |
Desalting capacity | > 85% | > 85% | > 90% | > 90% | > 90% |
Plate/column material | polypropylene and polyethylene | polypropylene and polyethylene | polypropylene and polyethylene | polypropylene and polyethylene | polypropylene and polyethylene |
Storage solution | 20% ethanol | 0.15% KathonTM | 0.15% Kathon | 0.15% Kathon | 0.15% Kathon |
Storage temperature | 4°C to 30°C | 4°C to 30°C | 4°C to 30°C | 4°C to 30°C | 4°C to 30°C |
1 Applied volume = eluted volume
2 Biomolecule depende
A pointed forceps is the best tool to use. The gel will be disturbed as the filter has to be tilted to remove it from the column tube. After removal of the filter, it is then best to let the column settle for approximately 10 min before use.