The use of a permeate flow control is strongly recommended during upstream clarification of target material. Permeate flow control rate is dependent on the process flux (LMH) and surface area. 

For permeate flow control you need a permeate pump kit. Detailed information regarding permeate pump kit for GrandStand 450 (GSM450PERPRP) and for GrandStand 550 (GSM550PERPRP) is found below.

Permeate side connections are not included with the GrandStand system. Please attach only light weight plastic fittings to the permeate side. To prevent possible damage to the cartridge, do not attach stainless steel valves directly to the permeate port.

To blank off the permeate, feed and retentate ports of the cartridge, when stored off the system:

Blank off cap 50 mm TC in stainless steel incl. gasket (44-7135-01)

Blank off cap 50 mm TC in plastic (56-4109-90)

Clamp 50 mm TC in stainless steel (12-4100-30) or plastic (56-4106-68)

Gasket o.d. 50 mm i.d. 38 mm, 1.5 inch (12-4100-34)


Please note that the item numbers are not necessarily in sequential order.

When stored off the system, store the cartridge with storage solution using blank off cap, gasket and clamp for each port.

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# Product Name Product Code Price
3 Clamp 50 mm (1") TC 12410030 15.39 USD Add to cart
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3 Clamp 50 mm TC 56410668 75.77 USD Add to cart
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3 Clamp 50 mm (1.5") TC (Toggle Clamp) 56410669 75.77 USD Add to cart
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7 TC-Gasket 51 mm i.d. 10 mm, EPDM 18101288 189.42 USD Add to cart
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7 Blind Flange, 51 mm TC 44713501 101.82 USD Add to cart
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7 50 mm (1.5") TC Blank-Off Cap, polysulfone 56410990 86.43 USD Add to cart
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8 50 mm (1.5") TC 13 mm (0.5") Barb 56410986 131.41 USD Add to cart
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8 50 mm TC i.d. 38 mm (1.5") 19 mm (0.75"ch) Barb 56410987 131.41 USD Add to cart
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Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General Advice for Troubleshooting when Using Cross Flow Filters

Please check the following items during troubleshooting:

Samples volume and system working volume have been used in calculations of concentration factor and diafiltration beforehand.

Process settings of Membrane Capacity, Flux, Shear (or volumetric cross flow rate) have been properly applied for both sample characteristics and membrane pore size or NMWC designation.

Filter is properly wetted before use (also make sure that there is no air lock in the filters created by condensation on microfiltration membranes as a result of incorrect autoclave settings).

Ensure CFF filters are properly wetted before starting processing. This may be tested against new cartridge “Clean Water Flux Test or against the water flux specifications in the Certificate of Quality. Microfitlration membranes are shipped dry and are rewetted with water or buffer. Hollow fiber ultrafiltration membranes are shipped in glycerol wetting preservative. These filters may require 15-20% alcohol/water solution to restore wetting or remove glycerol. Flat sheet cassette ultrafiltration devices (for example, KvickLab and KvickFlow Cassettes) are shipped in a solution of NaOH/Glycerol which must be flushed out before use.

Prior to using cassettes, make sure the single gasket is in the correct position in the holding device and that the cassette(s) within are oriented properly in the holder.

Check integrity of cassette system using water under pressure on retentate side to 1 barg to check for leaks in the seal of the cassette with the stainless steel holding device.

Check to see that cartridges or cassettes that have been previously used are checked for integrity (air diffusion or bubble point tests) and for cleanliness (normalized Clean Water Permeability before processing).