Accessories

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# Product Name Product Code Price
Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 103.32 USD Add to cart
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Filter Tool 18115320 141.14 USD Add to cart
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Tricorn Storage/Shipping Device 18117643 118.34 USD Add to cart
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Tricorn 5 Filter Kit 29053586 165.03 USD Add to cart
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Consumables

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# Product Name Product Code Price
Gel Filtration LMW Calibration Kit 28403841 708.96 USD Add to cart
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Gel Filtration HMW Calibration Kit 28403842 708.96 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before using the column make sure that:

  • Correct system has been selected in UNICORN System Control
  • Correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected and tubing is not longer than needed
  • All connectors are free from leakage, verified by passing a leakage test
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Column meets pressure requirements for selected medium
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

 

 

Superdex 200 Increase 5/150 GL

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