FAQ

Accessories

Running Prepacked Tricorn columns with ÄKTAexplorer, ÄKTAFPLC, ÄKTAprime plus, ÄKTApurifier and ÄKTAxpress.

 

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

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# Product Name Product Code Price
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" 18111253 88.79 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" 18111368 67.62 USD Add to cart
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1 Tubing i.d. 0.25 mm, o.d. 1/16" 18112095 60.50 USD Add to cart
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2 Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 113.65 USD Add to cart
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Running Prepacked Tricorn with ÄKTAmicro.

 

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.15 mm, o.d.1/16" 18115659 410.00 USD Add to cart
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2 Fingertight Narrow Head Connector, o.d. 1/16" 18114710 481.00 USD Add to cart
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# Product Name Product Code Price
1 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
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1 Outlet tubing P6000 18454601 47.18 USD Add to cart
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1 Unflanged tubing i.d. 1.2 mm, o.d. 1.8 mm 19437002 505.00 USD Add to cart
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3 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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3 Tubing, 2 m i.d. 0.8 mm, o.d. 1.8 mm, ETFE 19743501 68.66 USD Add to cart
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4 Pre-filter 6000 18458201 1 111.00 USD Add to cart
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4 Filter kit 18458401 129.59 USD Add to cart
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4 Holder Valve PSV-50 19199255 137.32 USD Add to cart
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4 Solenoid Valve PSV-50 19199401 744.00 USD Add to cart
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7 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
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8 UV Flow Cell, 2 mm 18111110 2 361.00 USD Add to cart
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8 Fiber Detachment Tool 18111116 119.80 USD Add to cart
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9 Filter 215 nm 11000733 744.00 USD Add to cart
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9 Filter 260 nm 11000734 710.00 USD Add to cart
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9 Filter 280 nm 11000735 519.00 USD Add to cart
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9 Filter 405 nm 11000736 679.00 USD Add to cart
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9 Empty Filter Holder 11000738 222.00 USD Add to cart
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9 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 9 825.00 USD Add to cart
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9 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
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9 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
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9 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
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9 O-ring kit 28969705 133.83 USD Add to cart
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Spare parts

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# Product Name Product Code Price
1 Filter Tool 18115320 141.14 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Unusual column appearance

Column leakage

Unsatisfactory elution

Poor product recovery

General advice to achieve good performance

Before using the column make sure that:

  • Correct system has been selected in UNICORN System Control
  • Correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected and tubing is not longer than needed
  • All connectors are free from leakage, verified by passing a leakage test
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Column meets pressure requirements for selected medium
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

 

 

Back pressure increase during operation

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