Superose 6 Increase 10/300 GL
Size exclusion chromatography columns: for high-resolution protein purification and analysis in MW range Mr~ 5K to ~ 5M.
Consumables
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| Gel Filtration LMW Calibration Kit | 28403841 | 708.96 USD |
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|
| Gel Filtration HMW Calibration Kit | 28403842 | 708.96 USD |
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Accessories
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 113.65 USD |
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|
| Filter Tool | 18115320 | 141.14 USD |
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|
| Tricorn Storage/Shipping Device | 18117643 | 118.34 USD |
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|
| Tricorn 10 Filter Kit | 29053612 | 165.03 USD |
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Running Prepacked Tricorn columns with ÄKTAexplorer, ÄKTAFPLC, ÄKTAprime plus, ÄKTApurifier and ÄKTAxpress.
Make sure you have all connectors and tubing needed for running the column.
Depending on your system configuration and column length you may need extra long tubing to connect your column.
Please do not extend the tubing unnecessarily which results in a lower resolution.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 80.74 USD |
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| 1 | PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" | 18111253 | 88.79 USD |
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| 1 | PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" | 18111368 | 67.62 USD |
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| 1 | Tubing i.d. 0.25 mm, o.d. 1/16" | 18112095 | 60.50 USD |
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| 2 | Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 113.65 USD |
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Running Prepacked Tricorn with ÄKTAmicro.
Make sure you have all connectors and tubing needed for running the column.
Depending on your system configuration and column length you may need extra long tubing to connect your column.
Please do not extend the tubing unnecessarily which results in a lower resolution.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 80.74 USD |
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| 1 | PEEK Tubing, 2 m, i.d. 0.15 mm, o.d.1/16" | 18115659 | 410.00 USD |
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| 2 | Fingertight Narrow Head Connector, o.d. 1/16" | 18114710 | 481.00 USD |
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| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | TUBING FLANG 1,2X420 FINELINE | 18110220 | 61.37 USD |
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| 1 | Outlet tubing P6000 | 18454601 | 47.18 USD |
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| 1 | Unflanged tubing i.d. 1.2 mm, o.d. 1.8 mm | 19437002 | 505.00 USD |
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| 3 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 80.74 USD |
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| 3 | Tubing, 2 m i.d. 0.8 mm, o.d. 1.8 mm, ETFE | 19743501 | 68.66 USD |
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| 4 | Pre-filter 6000 | 18458201 | 1 111.00 USD |
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| 4 | Filter kit | 18458401 | 129.59 USD |
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| 4 | Holder Valve PSV-50 | 19199255 | 137.32 USD |
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| 4 | Solenoid Valve PSV-50 | 19199401 | 744.00 USD |
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| 7 | Fingertight Union 1/16" Male/M6 Female | 18111258 | 111.71 USD |
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| 8 | UV Flow Cell, 2 mm | 18111110 | 2 361.00 USD |
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| 8 | Fiber Detachment Tool | 18111116 | 119.80 USD |
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| 9 | Filter 215 nm | 11000733 | 744.00 USD |
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| 9 | Filter 260 nm | 11000734 | 710.00 USD |
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| 9 | Filter 280 nm | 11000735 | 519.00 USD |
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| 9 | Filter 405 nm | 11000736 | 679.00 USD |
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| 9 | Empty Filter Holder | 11000738 | 222.00 USD |
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| 9 | Monitor UVis-920 Excluding Filter and Flow Cell | 11000754 | 9 825.00 USD |
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| 9 | Short optical fibre kit, 200 mm | 18113485 | 788.00 USD |
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| 9 | Long optical fibre kit, 500 mm | 18113486 | 582.00 USD |
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| 9 | UV flow cell, i.d. 8 mm | 28966687 | 3 025.00 USD |
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| 9 | O-ring kit | 28969705 | 133.83 USD |
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Spare parts
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Filter Tool | 18115320 | 141.14 USD |
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| 1 | Tricorn 10 Filter Kit | 29053612 | 165.03 USD |
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Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
General advice to achieve good performance
Before using the column make sure that:
- Correct system has been selected in UNICORN System Control
- Correct wavelength has been set for UV/UPC monitor
- All tubing has been properly connected and tubing is not longer than needed
- All connectors are free from leakage, verified by passing a leakage test
- No tubing is folded or twisted
- Online filter, if used, is changed on a regular basis
- Correct buffers are used for the chosen columns and proteins
- All inlet tubing has been immersed in correct buffer solutions
- Enough buffer has been prepared
- Buffers have been equilibrated to the environment temperature
- Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
- Suitable columns have been selected for the target proteins
- Column meets pressure requirements for selected medium
- Columns have been cleaned and prepared according to column instructions
- Samples have been clarified by centrifugation and/or filtration prior to sample loading
- Samples have been adjusted to binding buffer conditions
- Auto sampler (if used) has been prepared according to user manual
- The fraction collector has been filled with appropriate number of microtiter plates or tubes
- Appropriate arrangement for waste handling has been prepared
Superdex 75, Superdex 200 Increase and Superose 6 Increase
| Possible cause | Suggested remedy |
|---|---|
Increased back-pressure over the column and/or loss of resolution. |
Confirm that the column is the cause (see below). If so, clean it according to the procedure described in the section Cleaning in place (CIP). To confirm that the high back-pressure in the system is caused by the column, disconnect one piece of equipment at a time (starting at the fraction collector) with the pumps running. Check the pressure reading after each piece has been disconnected to determine the source of the back-pressure. |
| Possible cause | Suggested remedy |
|---|---|
Air in the column. |
Note that small amounts of air will normally not affect the performance of the column. Run 3 to 4 column volumes (CV) of well de-gassed eluent in an upflow direction at a flow rate of 0.2 ml/min (5/150) or 0.5 ml/min (10/300) at room temperature. |
| Possible cause | Suggested remedy |
|---|---|
Space between gel bed and adapter. |
Turn down the adapter to the gel bed. Perform a Column performance control. |