FAQ

Spare parts

Tricorn 10

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# Product Name Product Code Price
1 Tricorn 10 Adapter Unit 28406407 174.80 USD Add to cart
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2 Glass Tube 10/20 18115313 375.65 USD Add to cart
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2 Glass Tube 10/50 18115314 386.51 USD Add to cart
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2 Glass Tube 10/100 18115315 416.91 USD Add to cart
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2 Glass Tube 10/150 18115316 431.02 USD Add to cart
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2 Glass Tube 10/200 18115317 524.39 USD Add to cart
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2 Glass Tube 10/300 18115318 587.36 USD Add to cart
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2 Glass Tube 10/600 18115319 787.13 USD Add to cart
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3 Tricorn 10 Bottom Unit 18115310 142.23 USD Add to cart
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4 Tricorn O-Ring Packing Kit 18115282 76.00 USD Add to cart
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4 Tricorn O-Ring, 7 × 1 mm 18115312 264.91 USD Add to cart
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5 Stop Plug 1/16" Male 18111252 54.56 USD Add to cart
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6 Tricorn 10 Coarse Filter Kit 11001254 131.37 USD Add to cart
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Tricorn Storage/Shipping Device 18117643 118.34 USD Add to cart
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O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can   affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets.

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for the code numbers refer to the table below.

It could be useful to have unflanged tubing in stock, that could be cut in suitable length and flanged, to replace any damaged tubing.

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# Product Name Product Code Price
1 Tricorn O-Ring Packing Kit 18115282 76.00 USD Add to cart
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2 Tricorn O-Ring, 7 × 1 mm 18115312 264.91 USD Add to cart
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4 Tricorn 10 Coarse Filter Kit 11001254 131.37 USD Add to cart
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Accessories

Running Tricorn columns with ÄKTAexplorer, ÄKTAFPLC, ÄKTAprime plus and ÄKTApurifier.

 

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

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# Product Name Product Code Price
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" 18111253 88.79 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" 18111368 67.62 USD Add to cart
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1 Tubing i.d. 0.25 mm, o.d. 1/16" 18112095 60.50 USD Add to cart
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2 Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 113.65 USD Add to cart
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Make sure you have all connectors and tubing needed for packing and running the column.

Depending on your system configuration and column length, you may need extra long

tubing to connect your column.

Gelfiltration chromatography movies

 

Exchange of buffer

Preparing the column

Packing the column

Unpacking the columns

Adsorption chromatography movies  

Equipment required

Exchange of buffer

Preparing the column

Packing the column

Unpacking the columns

 
Column evaluation movie  

Column evaluation

 

 

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# Product Name Product Code Price
1 PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" 18111253 88.79 USD Add to cart
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1 PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" 18111368 67.62 USD Add to cart
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2 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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2 Tubing, 2 m i.d. 0.8 mm, o.d. 1.8 mm, ETFE 19743501 68.66 USD Add to cart
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3 Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 113.65 USD Add to cart
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4 Union 1/16" Female/M6 Male 18111257 106.37 USD Add to cart
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5 Valve SRV-1 19214501 266.00 USD Add to cart
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7 Packing Equipment 10/100 18115325 469.02 USD Add to cart
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8 Tricorn O-Ring Packing Kit 18115282 76.00 USD Add to cart
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8 Tricorn O-Ring, 7 × 1 mm 18115312 264.91 USD Add to cart
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8 Packing Connector 5-10 18115322 76.00 USD Add to cart
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8 Packing Connector 10-10 18115323 76.00 USD Add to cart
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8 Tricorn O-Ring, 12 × 1 mm 18115326 357.20 USD Add to cart
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9 Glass Tube 10/20 18115313 375.65 USD Add to cart
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9 Glass Tube 10/50 18115314 386.51 USD Add to cart
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9 Glass Tube 10/100 18115315 416.91 USD Add to cart
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9 Glass Tube 10/150 18115316 431.02 USD Add to cart
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9 Glass Tube 10/200 18115317 524.39 USD Add to cart
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9 Glass Tube 10/300 18115318 587.36 USD Add to cart
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9 Glass Tube 10/600 18115319 787.13 USD Add to cart
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10 Stop Plug 1/16" Male 18111252 54.56 USD Add to cart
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# Product Name Product Code Price
1 Tricorn 10 Adapter Unit 28406407 174.80 USD Add to cart
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2 Tricorn O-Ring, 7 × 1 mm 18115312 264.91 USD Add to cart
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3 Tricorn O-Ring Packing Kit 18115282 76.00 USD Add to cart
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# Product Name Product Code Price
1 TUBING FLANG 1,2X420 FINELINE 18110220 61.37 USD Add to cart
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1 Outlet tubing P6000 18454601 47.18 USD Add to cart
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1 Unflanged tubing i.d. 1.2 mm, o.d. 1.8 mm 19437002 505.00 USD Add to cart
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3 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 80.74 USD Add to cart
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3 Tubing, 2 m i.d. 0.8 mm, o.d. 1.8 mm, ETFE 19743501 68.66 USD Add to cart
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4 Pre-filter 6000 18458201 1 111.00 USD Add to cart
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4 Filter kit 18458401 129.59 USD Add to cart
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4 Holder Valve PSV-50 19199255 137.32 USD Add to cart
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4 Solenoid Valve PSV-50 19199401 744.00 USD Add to cart
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7 Fingertight Union 1/16" Male/M6 Female 18111258 111.71 USD Add to cart
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8 UV Flow Cell, 2 mm 18111110 2 361.00 USD Add to cart
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8 Fiber Detachment Tool 18111116 119.80 USD Add to cart
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9 Filter 215 nm 11000733 744.00 USD Add to cart
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9 Filter 260 nm 11000734 710.00 USD Add to cart
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9 Filter 280 nm 11000735 519.00 USD Add to cart
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9 Filter 405 nm 11000736 679.00 USD Add to cart
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9 Empty Filter Holder 11000738 222.00 USD Add to cart
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9 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 9 825.00 USD Add to cart
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9 Short optical fibre kit, 200 mm 18113485 788.00 USD Add to cart
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9 Long optical fibre kit, 500 mm 18113486 582.00 USD Add to cart
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9 UV flow cell, i.d. 8 mm 28966687 3 025.00 USD Add to cart
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9 O-ring kit 28969705 133.83 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Poor product recovery

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Worrying peak shape

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Drifting baseline

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Poor reproducibility

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Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.
 
 

Leading peak Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As). 

Please be aware of that for small columns, less than 10 ml bed volume, the system dead volume has an impact on the column evaluation values.

Issues related to Poor packing evaluation result

General advice to achieve good performance

Before using the system make sure that:

  • The correct system has been selected in UNICORN System Control
  • The correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected
  • All connectors are free from leakage
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

Column leakage

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Unsatisfactory elution

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Back pressure increases during operation

Unusual column appearance

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Strange peaks

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