Accessories

Make sure you have all connectors and tubing needed for packing and running the column.
Depending on your system configuration and column length, you may need extra long tubing to connect your column.

Gelfiltration chromatography movies  

Exchange of buffer

Preparing the column

Packing the column

Unpacking the columns

Adsorption chromatography movies  

Equipment required

Exchange of buffer

Preparing the column

Packing the column

Unpacking the columns

 
Column evaluation movie  

Column evaluation

 

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# Product Name Product Code Price
1 FEP Tubing, 3 m, i.d. 1/16", o.d. 1/8" 18112116 67.62 USD Add to cart
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2 Connector for 1/8" tubing 18112117 183.85 USD Add to cart
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3 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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5 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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7 Packing reservoir RK 50 28989861 706.79 USD Add to cart
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Make sure you have all connectors and tubing needed for running the column.
Depending on your system configuration and column length you may need extra long tubing to connect your column.
Please do not extend the tubing unnecessarily which results in a lower resolution.

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.
# Product Name Product Code Price
1 FEP Tubing, 3 m, i.d. 1/16", o.d. 1/8" 18112116 67.62 USD Add to cart
Get Quote
2 Connector for 1/8" tubing 18112117 183.85 USD Add to cart
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3 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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5 Connector, 5/16" Female - 5/16" Female 18117351 221.00 USD Add to cart
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6 Stop Plug 5/16" Male 18111250 33.21 USD Add to cart
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Spare parts

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XK 50 Column

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# Product Name Product Code Price
1 Adapter XK 50 28989880 488.57 USD Add to cart
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4 FEP Tubing, 3 m, i.d. 1/16", o.d. 1/8" 18112116 67.62 USD Add to cart
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9 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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10 O-ring Adapter, XK 50 28978228 102.06 USD Add to cart
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11 Plunger for XK 50, AK 50, AK 50 FF 18875801 93.37 USD Add to cart
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12 Support Screen for Column, i.d. 50 mm 19066401 93.37 USD Add to cart
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13 Net for Columns, i.d 50 mm, 10 µm 18875901 90.11 USD Add to cart
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14 End Piece Complete, XK50 18879701 107.48 USD Add to cart
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17 XK 50/20 Tube 18100085 170.45 USD Add to cart
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17 XK 50/100 Tube 19050901 469.02 USD Add to cart
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17 XK 50/60 Tube 19052501 345.25 USD Add to cart
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17 XK 50/30 Tube 19132601 266.00 USD Add to cart
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18 XK 50, 60 cm Jacket 18001177 310.51 USD Add to cart
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18 XK 50, 30 cm Jacket 18001178 252.97 USD Add to cart
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18 XK 50, 20 cm Jacket 18100083 183.48 USD Add to cart
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18 XK 50, 100 cm Jacket 18876501 391.94 USD Add to cart
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O-rings and nets should be checked regularly for wear. Worn O-rings may not seal properly and over-used nets can   affect distribution. Inspect the O-rings and evaluate the height equivalent to a theoretical plate (HETP) and peak asymmetry factor (As) to prevent poor performance due to old nets.

We recommend you to change the nets and O-rings when needed or at least annually. Please keep a set of each on your site, for the code numbers refer to the table below.

It could be useful to have unflanged tubing in stock, that could be cut in suitable length and flanged, to replace any damaged tubing.

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# Product Name Product Code Price
1 Net for Columns, i.d 50 mm, 10 µm 18875901 90.11 USD Add to cart
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2 Support Screen for Column, i.d. 50 mm 19066401 93.37 USD Add to cart
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3 O-ring Adapter, XK 50 28978228 102.06 USD Add to cart
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4 FEP Tubing, 3 m, i.d. 1/16", o.d. 1/8" 18112116 67.62 USD Add to cart
Get Quote
5 Ferrule for o.d. 1/8" Tubing 18112118 134.04 USD Add to cart
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6 Accessory kit XK 50 28989981 251.88 USD Add to cart
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7 Holder for XK 50 column 18309460 305.08 USD Add to cart
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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Poor product recovery

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Worrying peak shape

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Drifting baseline

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Poor reproducibility

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Poor packing evaluation result

On this troubleshooting site, we refer to leading and tailing peaks as defined according to the following figures.
 
 

Leading peak Tailing peak

 
Experience shows that the best method of expressing the efficiency of a packed column is in terms of its height equivalent to a theoretical plate (HETP), reduced plate number (h) and its peak asymmetry factor (As). 

Please be aware of that for small columns, less than 10 ml bed volume, the system dead volume has an impact on the column evaluation values.

Issues related to Poor packing evaluation result

General advice to achieve good performance

Before using the system make sure that:

  • The correct system has been selected in UNICORN System Control
  • The correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected
  • All connectors are free from leakage
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

Column leakage

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Unsatisfactory elution

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Back pressure increases during operation

Unusual column appearance

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Strange peaks

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