CNBr-Activated Sepharose 4 Fast Flow is more rigid than CNBr-Activated Sepharose 4B which in turn improves the pressure/flow characteristics. Operating flow rate for CNBr-Activated Sepharose 4 Fast Flow is 150-250 cm/h* compared to 70-140 cm/h for CNBr-Activated Sepharose 4 B.
Another difference is the coupling capacity- CNBr-Activated Sepharose 4B has a capacity of 25 to 60 mg α-chymotrypsinogen/ml drained medium whereas CNBr-Activated Sepharose 4 Fast Flow has a capacity of 13 to 26 mg α -chymotrypsinogen/ml.
*Volumetric flow rate (ml/min)= [Linear flow rate (cm/h) × column cross-sectional area (cm2)]/60