FAQ
See the table below for differences among the products:
Products | HisTrap HP | HisTrap FF | HisTrap FF crude | HisTrap excel |
---|---|---|---|---|
Product codes | 17524701,17524705, 17524801,17524802, 17524805,29051021 |
17531901, 17531902, 17525501, 17525502 | 11000458, 11000459, 17528601, 17528602, 29048631 | 17371205, 17371206, 29048586 |
Base matrix | Ni Sepharose High Performance | Ni Sepharose 6 Fast Flow | Ni Sepharose 6 Fast Flow | Ni Sepharose excel |
Average bead size | 34 µm | 90 µm | 90 µm | 90 µm |
Binding capacity (mg /ml) | > 40 mg | Approx. 40 mg | Approx. 40 mg | > 10 mg |
Application | The high protein binding capacity and the small bead size ensures high-resolution and minimal sample dilution. | The flow properties of the highly cross-linked agarose matrix make it an excellent choice for purification scale-up. | Application of unclarified sample is possible; no need to filter the sample before loading. | Purification of histidine-tagged proteins from samples that can cause Ni stripping from the medium, e.g. secreted proteins in liquids containing chelators. |
The nickel ions are very strongly bound to the ligand and will remain bound to the ligand even after incubation with 1 M NaOH or 10 mM EDTA. Also after the extreme conditions of incubating with 100 mM EDTA for 24 hours, a lot of the nickel remains bound. Therefore, recharging with nickel after normal use or CIP, even after several normal runs, is not necessary.