FAQ
The products can be used for two application areas:
- enrichment of proteins/immunoprecipitation/pulldowns/immunoaffinity, several different terms are used for this type of preparation—enhancing the signal of a protein of interest from clarified cell lysates and biological fluids
- small-scale purification of antibodies—screening of clones or the purification of small quantities of antibodies
For details on the usage of the specific products, see the table below.
Product | Protein enrichment | Antibody Purification |
Ab SpinTrap | X | X |
Ab Buffer Kit | X | |
NHS HP SpinTrap | X | |
NHS HP Buffer Kit | X | |
Protein A HP SpinTrap | X | X |
Protein A HP MultiTrap | X | X |
Protein A/G HP SpinTrap Buffer Kit | X | |
Protein G HP SpinTrap | X | X |
Protein G HP MultiTrap | X | X |
Streptavidin HP SpinTrap | X | |
Streptavidin HP MultiTrap | X | |
Streptavidin HP Buffer Kit | X |
Reproducible enrichment of native proteins minimizes variation in sample preparation for quantitative and comparative expression studies. Standardized protocols optimized for downstream analysis such as electrophoresis and LC-MS. High enrichment capability ensures improves downstream detection. Includes protocols that support performance optimization, modification and troubleshooting
Immunospecific affinity sample preparation procedures are based on the interaction between an antibody or similar molecule and its’ ligand protein. This is a platform for seven protein enrichment products with the flexibility of coupling an already established affinity molecule of a wide variety of types and also from a broad range of species. The affinity molecule on the column will be used as a bait to capture and enrich the proteins of interest in the sample with high specificity. The majority of the other proteins in the sample will be eliminated in flow through and wash fractions of the columns.
What are Streptavidin HP SpinTrap and Streptavidin HP MultiTrap?
Streptavidin HP SpinTrap is 16 prepacked spin columns packed with Streptavidin Sepharose High Performance medium. Streptavidin HP MultiTrap is a 4 pack with prepacked 96-well filter plates packed with Streptavidin Sepharose High Performance. Streptavidin Sepharose High Performance medium enable strong interaction to biotin.
Biotinylated affinity ligands (antibodies, proteins, aptamers and other molecules) immobilize tightly to the medium and the proteins of interest can then be enriched up to several hundred-fold depending of the ligand used.
What is the material used in SpinTrap?
Polypropylene and polyethylene.
What molecule is immobilized?
Streptavidin HP SpinTrap and MultiTrap:
Immobilization of biotinylated affinity ligands on antibodies, proteins, aptamers and other molecules.
What does the Streptavidin HP SpinTrap Buffer Kit contain?
The buffer kit consists of premade buffers for use with the protein enrichment protocol optimized for the Streptavidin HP SpinTrap. The stock solutions can be diluted directly into the provided bottles for convenient processing and storage. Some of the solutions are also provided in two bottles for increased storage. It contains solutions for 16 reactions.
Buffer | Content | Formulation | Volume |
Binding/washer buffer (TBS) | 0.5 M Tris; 1.5 M NaCl; | 10x | 2 x 5 ml |
pH 7.5 | |||
Elution buffer | 1 M glycine-HCl; pH 2.9 | 10x | 3 ml |
Blocking buffer | 2 mM biotin; 50 mM Tris; 150 mM NaCl; pH 7.5 |
5x | 2 ml |
What is the volume of a SpinTrap column?
800 μl column volume.
What is the pH stability of Streptavidin Sepharose High Performance?
pH 4-9 (long term), 2-10.5 (short term).
Note that a pH below pH 3 is sometimes required to elute strongly bound antibody species. However, protein ligands may hydrolyze at very low pH.
What is the medium volume in SpinTrap?
100 μl/column.
What is the maximum loading volume of SpinTrap?
600 μl/column
How do I store Streptavidin HP SpinTrap and Streptavidin HP MultiTrap?
Store at 4°C to 30°C in 20 % ethanol
How are the SpinTrap columns used?
SpinTrap columns are prepacked, single use spin columns that are used one by one or in parallel together with a bench top centrifuge for enrichment of proteins of interest.
What is the matrix for Streptavidin Sepharose High Performance?
The matrix is a highly cross-linked spherical 6% agarose, with the streptavidin ligand coupled with NHS-activation. The mean particle size is 34 μm.
Which sample sources can the products be used for?
The sample sources can come from a wide variety such as bacteria, cell lines, yeast, plant tissues, somatic fluids and tissue biopsies.