FAQ
The products can be used for two application areas:
- enrichment of proteins/immunoprecipitation/pulldowns/immunoaffinity, several different terms are used for this type of preparation—enhancing the signal of a protein of interest from clarified cell lysates and biological fluids
- small-scale purification of antibodies—screening of clones or the purification of small quantities of antibodies
For details on the usage of the specific products, see the table below.
Product | Protein enrichment | Antibody Purification |
Ab SpinTrap | X | X |
Ab Buffer Kit | X | |
NHS HP SpinTrap | X | |
NHS HP Buffer Kit | X | |
Protein A HP SpinTrap | X | X |
Protein A HP MultiTrap | X | X |
Protein A/G HP SpinTrap Buffer Kit | X | |
Protein G HP SpinTrap | X | X |
Protein G HP MultiTrap | X | X |
Streptavidin HP SpinTrap | X | |
Streptavidin HP MultiTrap | X | |
Streptavidin HP Buffer Kit | X |
Reproducible enrichment of native proteins minimizes variation in sample preparation for quantitative and comparative expression studies. Standardized protocols optimized for downstream analysis such as electrophoresis and LC-MS. High enrichment capability ensures improves downstream detection. Includes protocols that support performance optimization, modification and troubleshooting
What is the format of the 96-well filter plate and which standards does it comply with?
Prepacked 96-well filter plate size: 127.8 x 85.5 x 30.6 mm in compliance with ANSI/SBS1 1-2004, ANSI/SBS 3-2004 and ANSI/SBS 4- 2004.
1 ANSI= American National Standards Institute, SBS=Society for Biomolecular Screening
Is a collection plate available?
Yes, a 500 μl V-shaped 96-well collection plate (5 pack)
What is the medium volume in the wells?
50 μl/well.
What is the matrix for Streptavidin Sepharose High Performance?
The matrix is a highly cross-linked spherical 6% agarose, with the streptavidin ligand coupled with NHS-activation. The mean particle size is 34 μm.
Immunospecific affinity sample preparation procedures are based on the interaction between an antibody or similar molecule and its’ ligand protein. This is a platform for seven protein enrichment products with the flexibility of coupling an already established affinity molecule of a wide variety of types and also from a broad range of species. The affinity molecule on the column will be used as a bait to capture and enrich the proteins of interest in the sample with high specificity. The majority of the other proteins in the sample will be eliminated in flow through and wash fractions of the columns.
What are Streptavidin HP SpinTrap and Streptavidin HP MultiTrap?
Streptavidin HP SpinTrap is 16 prepacked spin columns packed with Streptavidin Sepharose High Performance medium. Streptavidin HP MultiTrap is a 4 pack with prepacked 96-well filter plates packed with Streptavidin Sepharose High Performance. Streptavidin Sepharose High Performance medium enable strong interaction to biotin.
Biotinylated affinity ligands (antibodies, proteins, aptamers and other molecules) immobilize tightly to the medium and the proteins of interest can then be enriched up to several hundred-fold depending of the ligand used.
What is the material used in MultiTrap?
Polypropylene and polyethylene.
What molecule is immobilized?
Streptavidin HP SpinTrap and MultiTrap:
Immobilization of biotinylated affinity ligands on antibodies, proteins, aptamers and other molecules.
Which sample sources can the products be used for?
The sample sources can come from a wide variety such as bacteria, cell lines, yeast, plant tissues, somatic fluids and tissue biopsies.
What is the well volume in MultiTrap?
800-μl deep-well volume.
What is the pH stability of Streptavidin Sepharose High Performance?
pH 4-9 (long term), 2-10.5 (short term).
Note that a pH below pH 3 is sometimes required to elute strongly bound antibody species. However, protein ligands may hydrolyze at very low pH.
How are the MultiTrap 96-well filter plates used?
MultiTrap is a prepacked, single use 96-well filter plate that enables enrichment of proteins in parallel. MultiTrap is used together with a centrifuge or vacuum manifold manually or automated in a robotic system.
What is the maximum loading volume of the wells?
600 μl/well.
Can MultiTrap 96-well filter plate be ordered empty?
No, MultiTrap can only ordered prepacked with medium.
What is the material used in the collection plate?
Polypropylene
How do I store Streptavidin HP SpinTrap and Streptavidin HP MultiTrap?
Store at 4°C to 30°C in 20 % ethanol
In what way is the plate delivered to the customer?
The plate delivered in 4 packs foiled at top and bottom of each plate. Each well contains 500 μl 10% slurry of medium in storage solution (50 μl medium in 450 μl 20% ethanol solution).