FAQ
Reproducible enrichment of native proteins minimizes variation in sample preparation for quantitative and comparative expression studies. Standardized protocols optimized for downstream analysis such as electrophoresis and LC-MS. High enrichment capability ensures improves downstream detection. Includes protocols that support performance optimization, modification and troubleshooting
Immunospecific affinity sample preparation procedures are based on the interaction between an antibody or similar molecule and its’ ligand protein. This is a platform for seven protein enrichment products with the flexibility of coupling an already established affinity molecule of a wide variety of types and also from a broad range of species. The affinity molecule on the column will be used as a bait to capture and enrich the proteins of interest in the sample with high specificity. The majority of the other proteins in the sample will be eliminated in flow through and wash fractions of the columns.
The products can be used for two application areas:
- enrichment of proteins/immunoprecipitation/pulldowns/immunoaffinity, several different terms are used for this type of preparation—enhancing the signal of a protein of interest from clarified cell lysates and biological fluids
- small-scale purification of antibodies—screening of clones or the purification of small quantities of antibodies
For details on the usage of the specific products, see the table below.
Product | Protein enrichment | Antibody Purification |
Ab SpinTrap | X | X |
Ab Buffer Kit | X | |
NHS HP SpinTrap | X | |
NHS HP Buffer Kit | X | |
Protein A HP SpinTrap | X | X |
Protein A HP MultiTrap | X | X |
Protein A/G HP SpinTrap Buffer Kit | X | |
Protein G HP SpinTrap | X | X |
Protein G HP MultiTrap | X | X |
Streptavidin HP SpinTrap | X | |
Streptavidin HP MultiTrap | X | |
Streptavidin HP Buffer Kit | X |
What does the Protein A/G HP SpinTrap Buffer Kit contain?
The buffer kit consists of premade buffers for use with the protein enrichment protocol optimized for the Protein A HP SpinTrap and the Protein G HP SpinTrap. It can also be used with the Ab SpinTrap. The stock solutions can be diluted directly into the provided bottles for convenient processing and storage. Some of the solutions are also provided in two bottles for increased storage. It contains solutions for 16 reactions.
Buffer | Content | Formulation | Volume |
Binding/washer buffer (TBS) | 0.5 M Tris; 1.5 M NaCl; pH 7.5 | 10x | 2 x 5 ml |
Elution buffer Classic | 2.5 % HAc | Ready to use | 20 ml |
Elution buffer Crosslink | 1 M glycine-HCl; pH 2.9 | 10x | 3 ml |
Crosslink solution A | 2 M triethanolamine; pH 8.9 | 10x | 4 ml |
Crosslink solution B | 1 M ethanolamine; pH 8.9 | 10x | 2 x 1 ml |
Note: DMP and urea (optional) is not in the kit provided for stability reasons.