ÄKTA avant 150
ÄKTA avant is a preparative chromatography system designed for fast and secure development of scalable methods and processes.
FAQ
Why should I have REGULAR, PLANNED MAINTENANCE on my system?
With the pressure on producing sample or results, the condition of your ÄKTAdesign or Ettan system is critical and regular servicing will mean you can depend on your system to perform as expected. Planned maintenance can be part of a service agreement, scheduled to service your system before it is in need of attention. We can help you design a schedule and routine to allow you to maintain your system, please contact your local Cytiva service representative.
So what can you expect from a planned maintenance visit from Cytiva service representative?
- Thorough inspection and cleaning of system components
- Update of system firmware to ensure full compatibility of your system and UNICORN software
- Replacement of damaged or corroded seals, valve springs and solenoids
- Replacement of items that are reaching the end of their expected life – preventing future breakdowns
- Advice and guidance on proper daily use, cleaning and care of your system
- All work is documented and reported to help make any regulatory audits easier.
A complete overhaul, once a year, ensures that your instrument is running at peak performance so you can be confident of your scientific results. In addition, wear and tear on systems under constant use by multiple end users is minimized, giving the system a longer life and better value for money.
To find out more about service possibilities contact your local Cytiva service representative.
| ÄKTA avant 25 | ÄKTA avant 150 |
|---|---|
| Columns | Columns |
| Tricorn 5, High Performance Column | HiScale 16 |
| Tricorn 10, High Performance Column | HiScale 26 |
| HiScale 16 | HiScale 50 |
| HiScale 26 | XK 16 |
| HiScale 50 | XK 26 |
| XK 16 | XK 50 |
| XK 26 | AxiChrom 50 |
| XK 50 | AxiChrom 70 |
ÄKTA avant
Chemical resistance guide and chemical compatibility
The chemical resistance of ÄKTA avant to some of the most commonly used chemicals in liquid chromatography is indicated in the table below.
The ratings are based on the following assumptions:
1. The synergistic effects of the chemical mixtures have not been taken into account.
2. Room temperature and limited over-pressure is assumed.
Note: Chemical influences are time and pressure dependent. Unless otherwise stated, all concentrations are 100%.
| Usage | Name | Concentration | CAS no and EEC no |
| Separation | Aqueous buffers, pH 2-12 |  |
|
|
Acetic acid | 5% | |
| Cleaning-In-Place (CIP) | Acetic acid | 70% | 64-19-7 200-580-7 |
| Reversed Phase Chromatography (RPC) | Acetonitrile Remarks: Quaternary valve not resistant. Depending on pressure, tubing between pump head, and pressure monitor needs to be changed. See section Prepare inlet tubing, in the User Manual. |
100% | 75-05-8 200-835-2 |
| RPC | Acetonitrile/Tetrahydrofuran (THF) Remarks: Quaternary valve not resistant. |
85/15 | 109-99-9 (Tetrahydrofuran) 203-726-8 |
| RPC | Acetone | 10% | |
| RPC | Acetone | 100% | 67-64-1 200-662-2 |
| Oligonucleotide synthesis | Ammonia | 30% | 7664-41-7 231-635-3 |
| Hydrophobic Interaction Chromatography (HIC) | Ammonium chloride | Saturated | 12125-02-9 235-186-4 |
| Purification of plasmids | Ammonium sulphate | 4 M | 7783-20-2 231-984-1 |
| Wash, using Protein A gels, Refolding | Arginine | 2 M | 74-79-3 200-811-1 |
| Cleaning and storage of columns | Benzyl alcohol | 2% | 100-51-6 202-859-9 |
| HIC | Citric acid | 100% | 29340-81-6 249-576-7 |
| Cleaning | Decon 90 | 10% | |
| RPC, CIP, Cell separation | Dimethyl sulphoxide (DMSO) | 5% | 67-68-5 200-664-3 |
| Cleaning, Plasmid separation | Dimethylformamide (DMF) Remarks: Quaternary valve not resistant |
100% | 1968-12-02 200-679-5 |
| Reducing agent | Dithiothreitol (DTT) | 0.1 M | 4413-31-4 NA |
| Reducing agent | DTE | 0.1 M | |
| Buffer additive | Ethylenediaminetetraacetic acid (EDTA) |
100% | 60-00-4 200-449-4 |
| CIP | Ethanol | 100% | 75-08-1 200-837-3 |
| Storage | Ethanol/Acetic acid | 20%/10% | |
| HIC | Ethylene glycol | 100% | 107-21-1 203-473-3 |
| HIC | Ethylene glycol | 100% | |
| RPC | Formic acid | 100% | 64-18-6 200-579-1 |
| HIC | Glycerol | 100% | 56-81-5 200-289-5 |
| Denaturing of proteins | Guanidinium hydrochloride | 6 M | |
| Cleaning of MAb binding media | Glycine | 2 M | 56-40-6 200-272-2 |
| CIP | Hydrochloric acid | 0.1 M | 7647-01-0 231-595-7 |
| CIP | Hydrochloric acid | 2 M | |
| Buffer preparation | Imidazole | 1 M | 288-32-4 206-019-2 |
| CIP | Isopropanol | 100% | 67-63-0 200-661-7 |
| RPC, CIP | Methanol | 100% | 74-93-1 200-659-6 |
| Reducing agent | Merkaptoethanole | 10% | 37482-11-4 253-523-3 |
| RPC | n-Propanol | 100% | 67-63-0 200-661-7 |
| CIP | Phosphoric acid | 10% | 7664-38-2 231-633-2 |
|
Potassium carbonate | Saturated | 584-08-7 209-529-3 |
| pH cell storage | Potassium chloride | Saturated | 7447-40-7 231-211-8 |
| Detergent | Sodium dodecyl sulfate (SDS) | 10% | 151-21-3 205-788-1 |
|
Sodium chloride | Saturated | 7647-14-5 231-598-3 |
| CIP | Sodium hydroxide | 2 M | 1310-73-2 215-185-5 |
| CIP | Sodium hydroxide | 1 M, 50°C | |
|
Sodium hydroxide + sodium hypochloride |
(1.0 M+500 ppm), 50°C |
|
| HIC | Sodium sulphate | Saturated | 7757-82-6 231-820-9 |
|
Trichloroacetic acid | 1% | 76-03-9 200-927-2 |
|
Trifluoroacetic acid | 1% | 176-05-1 200-929-3 |
| Detergent | Triton-X | 1% | 9002-93-1 (Triton X 100) |
| Detergent | Tween | 1% | 9005-64-5 (Tween 20) 500-018-3 |
| Buffer additive | Urea | 8M | 57-13-6 200-315-5 |
|
Water | 100% | 7732-18-5 231-791-2 |
How can I determine the delay volume?
Three different methods are described below:
Method I - Determining the delay volume of your system by measuring different retention volumes
| 1) Check that the pump is delivering the correct flow at 1 ml/min. If the measured flow rate differs the retention volumes need to be corrected. 2) Mount a small loop, e.g. 100 µl, and prepare a solution of 5% acetone in water to use as sample. 3) Fill the system with clean water. Run manually or make your own method. Start the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 1. 4) Re-configure your system. Dismount the tubing from the UV flow cell and insert a low dead volume connector, e.g. a female-female Valve connector, in the flow path (replaces the UV flow cell). Mount the “tubing end” of the frac outlet into the UV flow cell and mount a waste-tubing to the bottom of the UV flow cell. 5) Set Frac size to a very large volume, e.g. 100 ml, so that the valve is in the Frac position during the entire run. Start the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 2. Delay volume = Volume 2 – Volume 1. |
Method II - Determining the delay volume of your system with the help of another ÄKTA system
| This method is more accurate than the one described above, but demands one more system. 1) Check that the pump on your chosen ÄKTA system is delivering the correct flow at 1 ml/min. If the measured flow rate differs the retention volumes need to be corrected. 2) Mount a small loop, e.g. 100 µl, and prepare a solution of 2-5% acetone to use as sample. 3) Fill the system with clean water. Run manually or make your own method. Start the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 1. 4) Re-configure your system. In e.g. an ÄKTAprime system dismount the tubing from the UV flow cell to the Fraction collector including the restrictor, the Cond cell and the Frac valve. In the other ÄKTA system, e.g. ÄKTApurifier or ÄKTAexplorer, mount the “prime tubing kit” between the tubing to the UV flow cell and the UV flow cell. To do this a low dead volume connector, e.g. a female-female Valve connector, is needed. 5) In order to have the correct flow path the Frac valve in the “prime tubing kit” must be activated. This can be achieved be connecting it to the Valve B port of the P-900 pump in the ÄKTApurifier or ÄKTAexplorer system. The valve is activated by setting Pump B inlet to the B2 position. 6) Start the pump at 1 ml/min and inject the sample. The measured retention volume is called Volume 2. Delay volume = Volume 2 – Volume 1. |
Method III - Determining the delay volume by balancing eluted water
| Manually set the flow path to the direction of the fraction collector. Unscrew the tubing that is connected to inlet of the UV flow cell and insert a luer adaptor instead. Fill a syringe with water and inject water into flow cell unless it drops at the outlet of the fraction collector (in which case you have likely exceeded the pressure in the tubing which might be more than 4 bar, depending on configuration and flow restrictor used). Now fill the syringe with air (at least 20 ml because of compression) and displace the water. Collect eluting water in a small cup. Determine the system delay volume by balancing the cup before and after elution. Repeat two times for calculation of a mean value. Enter the mean value in "system settings" in UNICORN. |
I want to know the DIMENSIONS and WEIGHT of my system.
| System | Height (mm) |
Footprint (mm x mm) |
Weight (kg) |
Flow rate (ml/min) |
Pressure limit (MPa) |
|---|---|---|---|---|---|
| ÄKTA avant 25 | 660 | 860 x 710 | 116 | 0.001-25 | 20 |
| ÄKTA avant 150 | 660 | 860 x 710 | 116 | 0.001-150 (normal range) 0.001-300 (column packing flow) |
5 |
| ÄKTA pure | 630 | 535 x 470 | up to 53 kg | 0.001 to 25 (up to 50 during column packing) |
20 |
| ÄKTA pure 150 | 630 | 535 x 470 | up to 53 kg | 0.01 to 150 (up to 300 during column packing) |
5 |
| ÄKTAexplorer 10 | 620 | 500 x 460 | 75 | 0.001-10 | 25 |
| ÄKTAexplorer 100 | 620 | 500 x 460 | 75 | 0.01-100 | 10 |
| ÄKTAFPLC | 470 | 380 x 480 | 50 | 0.05-20 | 5 |
| ÄKTAmicro | 610 | 480 x 450 | 55 | 0.001-2 | 35 |
| ÄKTApilot | 900 | 750 x 540 | 114 | 4-400 (full gradients) 4-800 (limited gradients) |
2 |
| ÄKTAprime plus | 530 | 400 x 450 | 13 | 0.1-50 | 1 |
| ÄKTApurifier 10 | 620 | 500 x 460 | 75 | 0.001-10 | 25 |
| ÄKTApurifier 100 | 620 | 500 x 460 | 75 | 0.01-100 | 10 |
| ÄKTAxpress | 660 | 490 x 250 | 30 | 0.1-65 | 3 |
| Ettan LC | 610 | 480 x 450 | 55 | 0.001-2 | 35 |
| Ettan MDLC | 710 | 700 x 640 | 105 | 0.001-2 | 35 |
| Ettan microLC | 1150 | 650 x 500 | 77 | 0.001-2 |
35
|
| Ettan nanoLC | 1150 | 650 x 500 | 77 | 0.001-2 | 35 |
User Documentation on DVD
ÄKTA avant and UNICORN 6.1 Installation Guide
Getting Started with ÄKTA avant and UNICORN 6.1
ÄKTA avant and UNICORN 6.1 User Manual
UNICORN 6 Method Manual
UNICORN 6 Evaluation Manual
UNICORN 6 Technical and Administration Manual
Other
EC Declaration of conformity
Certificate of conformance
How can I CLEAN my system?
ÄKTA avant
Cleaning the system
The procedures described below are for system cleaning.
1. Clean the UV flow cell
Clean the UV flow cell every six months, or when required.
NOTICE: Keep UV flow cell clean. Do not allow solutions containing dissolved salts, proteins or other solid solutes to dry out in the flow cell. Do not allow particles to enter the flow cell, as damage to the flow cell may occur.
Required material
The following material is required:
• Luer connector
• Waste container
• Syringe, 25-30 ml
• 10% surface active detergent solution (e.g., Decon 90, Deconex 11, or RBS 25)
• Distilled water
Instruction
Follow the instruction below to clean the UV flow cell. The UV flow cell can be either mounted or not mounted on the instrument during the cleaning procedure.
| Step | Action |
| 1 | Disconnect the fingertight connector and the tube from the top of the UV flow cell, and replace the fingertight connector with a Luer connector. |
|
 |
| 2 | Disconnect the tube from the bottom of the UV flow cell, and connect a waste tube to the UV flow cell. Immerse the waste tube in a waste container. |
| 3 | Fill a syringe with distilled water, and connect the syringe to the Luer connector. |
|
 |
| 4 | Squirt the distilled water through the UV flow cell in small amounts. Disconnect the syringe. |
| 5 | Fill a syringe with a 10% surface active detergent solution like Decon 90, Deconex 11, RBS 25 or equivalent, and connect the syringe to the Luer connector. |
| 6 | Squirt the detergent solution through the UV flow cell about five times. |
| 7 | Leave the detergent solution in the flow cell for at least 20 minutes. |
| 8 | Inject the detergent solution remaining in the syringe into the flow cell. Disconnect the syringe. |
| 9 | Fill a syringe with distilled water. Connect the syringe to the Luer connector. |
| 10 | Inject the distilled water into the UV flow cell to rinse the flow cell. Disconnect the syringe. |
| 11 | Disconnect the Luer connector from the top of the UV flow cell, and reconnect the fingertight connector with the tube. |
| 12 | Disconnect the waste tubing from the bottom of UV flow cell. Reconnect the piece of tubing from the Conductivity monitor to the bottom of the UV flow cell. |
2. Clean the instrument externally
Clean the the instrument externally when required. Do not allow spilled liquid to dry on the instrument.
Required material
The following material is required:
• Cloth
• Mild cleaning agent or 20% ethanol
Instruction
Follow the instruction below to clean the instrument externally.
| Step | Action |
| 1 | Check that no run is in progress. |
| 2 | Switch off the instrument. |
| 3 | Wipe the surface with a damp cloth. Wipe off stains using a mild cleaning agent or 20% ethanol. Wipe off the excess. |
| 4 | Let the instrument dry completely before using it. |
3. Perform System CIP, Cleaning-in-place
Perform a System CIP when required, for example between runs where different samples and buffers are used. This is important to prevent bacterial growth in the instrument.
Required material
The following material is required:
• Appropriate cleaning solutions (e.g., NaOH, buffer solution or distilled water).
• Syringe, 25-30 ml
Instruction
Follow the instruction in Section 7.6.2 Perform System CIP in the ÄKTA avant and UNICORN 6.1 User Manual.
4. Clean the Fraction collector
Maintenance interval
Clean the Fraction collector when required, for example if liquid has been spilled in the Fraction collector chamber.
Required material
The following material is required:
• Wash bottle
• Water or 20% ethanol
• Cloth
Instruction
Follow the instruction below to clean the interior of the Fraction collector. See the locations of the components of the Fraction collector in Section 3.4.1 Illustrations of the Fraction collector, in the ÄKTA avant and UNICORN 6.1 User Manual.
| Step | Action |
| 1 | In System Control, select Manual:Execute Manual Instructions:Fraction collection:Frac cleaning position. Click Execute. Result: The Dispenser head moves to cleaning position, and the Instrument display states System pause. |
| 2 | Open the Frac drawer and lift off the Cassette tray. |
| 3 | Wash the Cassettes and the Cassette tray with water and a mild cleaning agent. |
| 4 | Lift off the Waste funnel and wash it with water and a mild cleaning agent. Refit the Waste funnel. |
| 5 | In ÄKTA avant 150 only: Remove the Dispenser head cover and wash it with water and a mild cleaning agent. Refit the Dispenser head cover. |
| 6 | Wipe off the Frac chamber using a damp cloth. Wipe off stains using a mild cleaning agent or 20% ethanol. Note: Be careful not to damage the Arm flat cable. TIP: The Frac arm can easily be moved to facilitate cleaning of the Fraction collector. TIP: The Cassette tray positioning discs can easily be removed to facilitate cleaning of the Fraction collector. Make sure to refit the discs before the Cassette tray is inserted in the Frac chamber. |
| 7 | Wipe off the Dispenser head and its diode windows using a wash bottle with water or 20% ethanol and a cloth. ÄKTA avant 25 has four diode windows and ÄKTA avant 150 has two diode windows (see the illustrations below). Note: Be careful not to scratch the diode windows. |
| 8 | Let the Fraction collector dry completely before starting a run. |
| 9 | Close the Frac drawer. Result: Automatic scanning is performed. |
| 10 | In the System Control module, press the End icon in the toolbar. Result: The Dispenser head moves to home position, and the Instrument display states Ready. |
5. Clean the pH electrode
Clean the pH electrode when required. During cleaning, the pH electrode can be either installed in the pH valve, or not installed in the pH valve. The pH electrode has a limited longevity and should be replaced every six months or when the response time is slow, see Section 7.5.2 Replace the pH electrode, in the ÄKTA avant and UNICORN 6.1 User Manual. After cleaning has been performed, re-calibrate the pH monitor, see Section 7.7.1 Calibrate the pH monitor, in the ÄKTA avant and UNICORN 6.1 User Manual.
Required material
• Syringe, 25-30 ml
• Distilled water
• 0.1 M HCl and 0.1 M NaOH
Cleaning agents
Clean the pH electrode using one of the following procedures:
Salt deposits
Dissolve the deposits by immersing the electrode in:
• 0.1 M HCl
• 0.1 M NaOH
• 0.1 M HCl
each for five minutes. Rinse electrode tip in distilled water between each solution.
Oil or grease films
Wash the electrode tip in liquid detergent and water. If the films are known to be soluble in a particular organic solvent, wash with this solvent. Rinse the electrode tip in distilled water.
Protein deposits
Dissolve the deposit by immersing the electrode in a 1% pepsin solution in 0.1 M HCl for five minutes followed by thorough rinsing with distilled water. If these procedures fail to rejuvenate the electrode, try the following procedure.
Note: This procedure can be performed only when the pH electrode is not installed in the pH valve.
| Step | Action |
| 1 | Heat a 1 M KNO3 solution to 60°C–80°C. |
| 2 | Place the electrode tip in the heated KNO3 solution. |
| 3 | Allow the electrode to cool while immersed in the KNO3 solution before re-testing. |
6. Clean a pH electrode installed in the pH valve
| Step | Action |
| 1 | Open the System Control module and select System:Calibrate. Result: The Calibration dialog opens.  |
| 2 | Set Monitor to calibrate by selecting pH from the list. |
| 3 | Press the Prepare for calibration button. Result: The pH valve switches to the calibration position. |
| 4 | Fill a syringe with approximately 10 ml of chosen cleaning solution. Connect the syringe to the pH valve port Cal. Inject the liquid and wait for 5 minutes. Disconnect the syringe. |
| 5 | If several cleaning solutions are to be used, repeat step 4 with distilled water and then with the next solution. |
| 6 | As the last step in the cleaning procedure: • Fill a syringe with distilled water. • Connect the syringe to the pH valve port Cal. • Inject the water. • Disconnect the syringe. |
| 7 | Press the Close button. |
| 8 | Result: The pH valve switches back to the default position and the Calibration dialog closes. No calibration is performed. |
7. Clean the check valves of a pump head
Maintenance interval
Clean the check valves when required, for example if solids in the check valve cause irregular or low flow.
Required material
The following material is required:
• Adjustable wrench
• Methanol
• Distilled water
• Ultrasonic bath
Instruction
Follow the instruction below to remove and clean the check valves of the Sample pump or the system pumps.
| Step | Action |
| 1 | Switch off the instrument. |
| 2 | Disconnect the tubing from the pump head and disconnect the pump inlet tubing. Disconnect the tubing of the pump rinsing system. |
| 3 | Unscrew the purge valve by turning it counter-clockwise, and lift off the metal ring. |
| 4 | Unscrew the plastic nut of the upper check valve using an adjustable wrench, and gently lift off the upper check valve. |
| 5 | Unscrew the two white plastic screws located below each pump head. Pull the plastic connectors to the sides to release the inlet manifold. |
| 6 | Unscrew the lower check valve using an adjustable wrench. |
| 7 |  WARNING: Hazardous substances. When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe operation and maintenance of the system. Immerse the complete valves in methanol and place them in an ultrasonic bath for a few minutes. Repeat the ultrasonic bath with deionized water. |
| 8 | Refit the check valves. Follow the instructions below to fasten the cleaned check valve: • For black plastic valves: Tighten the nut until fully finger-tight and then use the adjustable wrench to tighten a further 90 degrees. • For metal valves: Tighten the nut until fully finger-tight and then use the adjustable wrench to tighten a further 90 degrees. |
| 9 | Refit the inlet manifold and reconnect the tubing to the pump head. |
8. Clean the conductivity flow cell
Clean the Conductivity flow cell when required.
Required material
The following material is required:
• Luer connector
• Waste container
• Syringe, 25-30 ml
• 1 M NaOH
• Distilled water
Instruction
Follow the instruction below to clean the flow cell of the Conductivity monitor.
| Step | Action |
| 1 | Disconnect the fingertight connector and the tube from the top of the Conductivity monitor, and attach a Luer connector. |
| 2 | Disconnect the tube from the bottom of the Conductivity monitor, and connect a waste tube to the Conductivity monitor. Immerse the waste tubing in a waste container. |
| 3 | Disconnect the Conductivity monitor from the rails, or slide the Conductivity monitor to the right on the rails. |
| 4 | Fill a syringe with distilled water, and connect the syringe to the Luer connector. |
| 5 | Squirt distilled water through the Conductivity flow cell in small amounts. Disconnect the syringe. |
| 6 |  WARNING: Hazardous substances. When using hazardous chemical and biological agents, take all suitable protective measures, such as wearing protective glasses and gloves resistant to the substances used. Follow local and/or national regulations for safe operation and maintenance of the system. Fill a syringe with 1 M NaOH, and connect the syringe to the Luer connector. |
| 7 | Squirt 1 M NaOH through the Conductivity flow cell about five times. |
| 8 | Leave the liquid in the flow cell for at least 15 minutes. |
| 9 | Fill a syringe with distilled water. Connect the syringe to the Luer connector. |
| 10 | Inject the distilled water into the Conductivity flow cell to rinse the flow cell. Disconnect the syringe. |
| 11 | Disconnect the Luer connector from the top of the Conductivity flow cell, and reconnect the fingertight connector with tubing. |
I want to know the FLOW and PRESSURE RANGE of my system.
| System | Height (mm) |
Footprint (mm x mm) |
Weight (kg) |
Flow rate (ml/min) |
Pressure limit (MPa) |
|---|---|---|---|---|---|
| ÄKTA avant 25 | 660 | 860 x 710 | 116 | 0.001-25 | 20 |
| ÄKTA avant 150 | 660 | 860 x 710 | 116 | 0.001-150 (normal range) 0.001-300 (column packing flow) |
5 |
| ÄKTA pure | 630 | 535 x 470 | up to 53 kg | 0.001 to 25 (up to 50 during column packing) |
20 |
| ÄKTA pure 150 | 630 | 535 x 470 | up to 53 kg | 0.01 to 25 (up to 300 during column packing) |
5 |
| ÄKTAexplorer 10 | 620 | 500 x 460 | 75 | 0.001-10 | 25 |
| ÄKTAexplorer 100 | 620 | 500 x 460 | 75 | 0.01-100 | 10 |
| ÄKTAFPLC | 470 | 380 x 480 | 50 | 0.05-20 | 5 |
| ÄKTAmicro | 610 | 480 x 450 | 55 | 0.001-2 | 35 |
| ÄKTApilot | 900 | 750 x 540 | 114 | 4-400 (full gradients) 4-800 (limited gradients) |
2 |
| ÄKTAprime plus | 530 | 400 x 450 | 13 | 0.1-50 | 1 |
| ÄKTApurifier 10 | 620 | 500 x 460 | 75 | 0.001-10 | 25 |
| ÄKTApurifier 100 | 620 | 500 x 460 | 75 | 0.01-100 | 10 |
| ÄKTAxpress | 660 | 490 x 250 | 30 | 0.1-65 | 3 |
| Ettan LC | 610 | 480 x 450 | 55 | 0.001-2 | 35 |
| Ettan MDLC | 710 | 700 x 640 | 105 | 0.001-2 | 35 |
| Ettan microLC | 1150 | 650 x 500 | 77 | 0.001-2 |
35
|
| Ettan nanoLC | 1150 | 650 x 500 | 77 | 0.001-2 | 35 |
Flow path schemes
Flow path scheme ÄKTAavant 150
Inlet tubing
The table below shows the labels, diameters and the standard lengths of the inlet tubing. The inlet tubing is mounted on delivery.
| Label | Description | Tubing | Length (mm) |
|---|---|---|---|
| A1 - A7 | Inlets to Inlet valve A | PTFE, o.d. 3/16", i.d. 2.9 mm | 1500 |
| B1 - B7 | Inlets to Inlet valve B | PTFE, o.d. 3/16", i.d. 2.9 mm | 1500 |
| Q1 -Q4 | Inlets to Quaternary valve | PTFE, o.d. 1/8", i.d. 1.6 mm | 1500 |
| Buff | Buffer inlet to Sample inlet valve | PTFE, o.d. 3/16", i.d. 2.9 mm | 1500 |
| S1 - S7 | Inlets to Sample inlet valve | PTFE, o.d. 3/16", i.d. 2.9 mm | 1500 |
| InQA | From Quaternary valve to Inlet valve A | PTFE, o.d. 1/8", i.d. 1.6 mm | 110 |
| InQB | From Quaternary valve to Inlet valve B | PTFE, o.d. 1/8", i.d. 1.6 mm | 110 |
| InA | From Inlet valve A to System pump A | PTFE, o.d. 3/16", i.d. 2.9 mm | 220 |
| InB | From Inlet valve B to System pump B | PTFE, o.d. 3/16", i.d. 2.9 mm | 220 |
| InS | From Sample inlet valve B to Sample pump | PTFE, o.d. 3/16", i.d. 2.9 mm | 220 |
High pressure tubing
The table below shows the labels, diameters and the standard lengths of the high pressure tubing. The high pressure tubing is mounted on delivery.
| Label | Description | Tubing | Length (mm) |
|---|---|---|---|
| 1A1 | System pump A left to Restrictor A | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 1A2 | System pump A right to Restrictor A | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 2A | Restrictor A to Pressure monitor | PEEK, o.d. 1/16", i.d. 1.0 mm | 100 |
| 1B1 | System pump B left to Restrictor B | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 1B2 | System pump B right to Restrictor B | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 2B | Restrictor B to Pressure monitor | PEEK, o.d. 1/16", i.d. 1.0 mm | 100 |
| 1S1 | Sample pump left to Restrictor S | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 1S2 | Sample pump right to Restrictor S | PEEK, o.d. 1/16", i.d. 1.0 mm | 340 |
| 2S | Restrictor S to Pressure monitor | PEEK, o.d. 1/16", i.d. 1.0 mm | 100 |
| 3 | Pressure monitor to Mixer | PEEK, o.d. 1/16", i.d. 1.0 mm | 280 |
| 3S | Pressure monitor S to Injection valve | PEEK, o.d. 1/16", i.d. 1.0 mm | 485 |
| 4 | Mixer to Injection valve | PEEK, o.d. 1/16", i.d. 1.0 mm | 400 |
| InjEF | Bypass tubing in injection valve | PEEK, o.d. 1/16", i.d. 0,75 mm | 200 |
| 5 | Injection valve to Column valve | PEEK, o.d. 1/16", i.d. 1.0 mm | 180 |
| 6 | Column valve to UV monitor | PEEK, o.d. 1/16", i.d. 1.0 mm | 170 |
| 7 | UV monitor to Conductivity monitor | PEEK, o.d. 1/16", i.d. 1.0 mm | 100 |
| 8 | Conductivity monitor to pH valve | PEEK, o.d. 1/16", i.d. 1.0 mm | 165 |
| 9 | pH valve to Outlet valve | PEEK, o.d. 1/16", i.d. 1.0 mm | 235 |
| 1R | To Flow restrictor | PEEK, o.d. 1/16", i.d. 0.75 mm | 75 |
| 2R | From Flow restrictor | PEEK, o.d. 1/16", i.d. 0.75 mm | 75 |
| Frac | Outlet valve to fraction collector | PEEK, o.d. 1/16", i.d. 1.0 mm | 1310 |
Reference capillaries
The table below shows the labels, diameters and the standard lengths of the reference capillaries.
| Label | Description | Tubing | Length (mm) |
|---|---|---|---|
| Ref 1 | Reference capillary | PEEK, o.d. 1/16", i.d. 0.25 mm | 400 |
Outlet tubing
The table below shows the labels, diameters and the standard lengths of the outlet tubing.
| Label | Description | Tubing | Length (mm) |
|---|---|---|---|
| Out1 - Out 32 | Outlets from Outlet valve | PTFE, o.d. 1/8", i.d. 1.6 mm | 1000 |
Waste tubing
The table below shows the labels, diameters and the standard lengths of the waste tubing. The waste tubing is mounted on delivery.
| Label | Description | Tubing | Length (mm) |
|---|---|---|---|
| W1 | System pump waste | o.d. 1/16", i.d. 1.0 mm | 1900 |
| W2 | Sample pump waste | o.d. 1/16", i.d. 1.0 mm | 1900 |
| W3 | pH valve waste | o.d. 1/16", i.d. 1.0 mm | 1900 |
| W | System waste | o.d. 1/16", i.d. 1.6 mm | 1900 |
| N/A | Top tray waste | o.d. 12 mm, i.d. 8 mm | 2500 |
| N/A | Frac front waste | o.d. 12 mm, i.d. 8 mm | 2100 |
| N/A | Frac back waste | o.d. 12 mm, i.d. 8 mm | 1600 |
|
Illustration of the liquid flow path
|
| UNICORN flow scheme During a run, the current liquid flow path is displayed in the Flow Scheme pane of the SystemControl module in UNICORN. Open flow paths are displayed in green and closed flow paths are displayed in grey. Red modules indicate alarms. Real-time data from monitors are also shown in the flow scheme. To view and/or issue instructions controlling a module, double-click the module in the flow scheme or right-click the module and select Instructions and the instruction of interest. The Manual Instructions dialog opens with the chosen instruction selected. To view the ports and the different flow paths of a valve, right-click the valve in the flow scheme and select Show Details. An illustration of ports and available flow paths opens. Illustration of UNICORN flow scheme The flow scheme displayed in UNICORN is shown in the illustration below.  Flow paths of Injection valve The Injection valve can be set to different positions that give different flow paths through the valve. The illustration and table below describe the different flow paths of the Injection valve. The method progressing flow, marked in the illustration below, defines the flow from which the volume base is calculated.   |
Accessories
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Jumper 1 IEC 1394 | 28956489 | 53.82 USD |
Add to cart
|
The illustration below shows the recommended positions for the extra air sensors.
Note: Only one extra air sensor can be installed.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Air Sensor L9-1.5 mm | 28956500 | 2,580.00 USD |
Add to cart
|
| 2 | Air Sensor L9-1.2 mm | 28956502 | 2,392.00 USD |
Add to cart
|
| Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 123.16 USD |
Add to cart
|
|
| PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" | 18111368 | 73.48 USD |
Add to cart
|
|
| FEP Tubing, 3 m, i.d. 1/16", o.d. 1/8" | 18112116 | 73.48 USD |
Add to cart
|
|
| Connector for 1/8" tubing | 18112117 | 199.76 USD |
Add to cart
|
|
| Ferrule for o.d. 1/8" Tubing | 18112118 | 145.94 USD |
Add to cart
|
|
| Adapter for air sensor | 28956342 | 171.81 USD |
Add to cart
|
Illustration of Fraction collector F9-R, connector panel, base unit and delivery arm.
| Part | Function |
|---|---|
| 1 | Lock knob |
| 2 | Delivery arm |
| 3 | Tubing connector |
| 4 | Tube sensor |
| 5 | Collection tubes |
| 6 | Tube rack |
| 7 | Base unit |
| 8 | Node switch |
| 9 | UniNet-9 communication and power supply |
| 10 | Central spindle |
| 11 | Drive sleeve |
| 12 | Delivery arm |
| 13 | Delivery arm |
| 14 | Tube adjustment cavity |
| 15 | Sensor control |
| 16 | Tubing holder |
| 17 | Tubing holder nut |
| 18 | Exposed tubing end length |
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| Tube Rack 40 × 30 mm, bowl, tube support, holder and guide | 18112467 | 609.00 USD |
Add to cart
|
|
| Tube Holder and Guide, 40 × 30 mm | 18112468 | 520.00 USD |
Add to cart
|
|
| Tube Rack Complete, 95 × 10–18 mm | 18305003 | 681.00 USD |
Add to cart
|
|
| Bowl | 18305103 | 205.00 USD |
Add to cart
|
|
| Tube Support | 18305402 | 154.21 USD |
Add to cart
|
|
| Tubing Holder | 18646401 | 99.36 USD |
Add to cart
|
|
| Drive sleeve | 19606702 | 133.07 USD |
Add to cart
|
|
| Tube Holder and Guide, 175 × 12 mm | 19724202 | 419.00 USD |
Add to cart
|
|
| Tube Rack 175 × 12 mm, bowl, tube support, holder and guide | 19868403 | 494.00 USD |
Add to cart
|
|
| Tube Holder and Guide, 95 × 10–18 mm | 19868902 | 275.00 USD |
Add to cart
|
|
| Fraction Collector F9-R | 29011362 | 4,270.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Mixer chamber 1.4 ml | 28956225 | 1,370.00 USD |
Add to cart
|
| 2 | Mixer chamber 5 ml | 28956246 | 1,370.00 USD |
Add to cart
|
| 3 | Mixer chamber 15 ml | 28980309 | 1,969.00 USD |
Add to cart
|
| 4 | O-ring 13.1x1.6 mm | 28953545 | 86.95 USD |
Add to cart
|
| 5 | O-ring 22.1 x 1.6 mm | 28981857 | 139.72 USD |
Add to cart
|
| 6 | Online filter kit | 18102711 | 144.90 USD |
Add to cart
|
Function of the I/O-box
The I/O-box E9 is used to interface other equipment in order to measure parameters such as refractive index, light scattering and fluorescence.
| Part | Description |
|---|---|
| 1 | I/O-box |
| 2 | D-sub connectors |
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| I/O-box E9 | 29011361 | 2,842.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Column Holder Rod | 28956270 | 1,017.00 USD |
Add to cart
|
| 2 | Tubing holder, spool, for small tubing | 28956274 | 83.84 USD |
Add to cart
|
| 3 | Column Holder | 28956282 | 138.69 USD |
Add to cart
|
| 4 | Tubing holder, comb | 28956286 | 83.84 USD |
Add to cart
|
| 5 | Flexible column holder | 28956295 | 1,480.00 USD |
Add to cart
|
| 6 | Column Clamp | 28956319 | 83.84 USD |
Add to cart
|
| 7 | Bottle holder | 28956327 | 306.00 USD |
Add to cart
|
| 8 | Adapter for air sensor | 28956342 | 171.81 USD |
Add to cart
|
| 9 | Tube holder | 28954329 | 52.78 USD |
Add to cart
|
| 10 | Rail Extension | 29011352 | 443.00 USD |
Add to cart
|
| 11 | Multi-Purpose Holder | 29011349 | 79.69 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | UniTag (sheet with 108 labels) | 28956491 | 96.26 USD |
Add to cart
|
The valves indicated in the figure below are mounted at delivery.
Additional valves used in optional configurations are also included in the order information list below the figure.
Figure. Valves.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 4 | Injection valve V9H-Inj | 28979283 | 3,995.00 USD |
Add to cart
|
| 7 | Inlet valve V9H-IS | 28979279 | 6,220.00 USD |
Add to cart
|
| Inlet Valve V9H-A2 | 28979303 | 6,516.00 USD |
Add to cart
|
|
| Inlet Valve V9H-B2 | 28979315 | 6,516.00 USD |
Add to cart
|
|
| Sample Inlet Valve V9H-S2 | 28979320 | 6,516.00 USD |
Add to cart
|
|
| Inlet Valve V9H-X1 | 28979326 | 4,398.00 USD |
Add to cart
|
|
| Inlet Valve V9H-X2 | 28979328 | 4,398.00 USD |
Add to cart
|
|
| Column Valve V9H-C2 | 28979330 | 8,690.00 USD |
Add to cart
|
|
| Outlet Valve V9H-O2 | 28979332 | 3,999.00 USD |
Add to cart
|
|
| Outlet Valve V9H-O3 | 28979337 | 3,999.00 USD |
Add to cart
|
|
| Loop valve kit V9H-L | 29090689 | 6,306.00 USD |
Add to cart
|
|
| Versatile valve V9H-V | 29090691 | 3,159.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Wet side waste tray | 28956487 | 497.00 USD |
Add to cart
|
| 2 | Front side waste tray | 28956485 | 418.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Cassette tray | 28954209 | 310.00 USD |
Add to cart
|
| 2 | Cassette for 50 ml tubes | 28956402 | 197.68 USD |
Add to cart
|
| 3 | Cassette, for 15 ml tubes | 28956404 | 197.68 USD |
Add to cart
|
| 4 | Cassette for fraction collection in 8 mL tubes | 28956425 | 198.72 USD |
Add to cart
|
| 5 | Cassette, for 3 ml tubes | 28956427 | 198.72 USD |
Add to cart
|
| 6 | Cassette for fraction collection in deep-well plate | 28954212 | 194.58 USD |
Add to cart
|
| 7 | Rack, for 50 ml tubes | 28980319 | 774.00 USD |
Add to cart
|
The Extension box can be mounted in two ways.
Standing on top of or next to ÄKTA avant.
On a Rail extension rod (29-0113-52) on ÄKTA avant.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| Dummy Module | 28956493 | 1,226.00 USD |
Add to cart
|
|
| Rail Extension | 29011352 | 443.00 USD |
Add to cart
|
|
| Extension Box | 29110806 | 505.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Flow Restrictor FR-902 | 18112135 | 758.00 USD |
Add to cart
|
The illustrations show tubing connections for ÄKTA avant 150.
The illustration to the left refers to Tubing kit, AxiChrom 50 -70 / ÄKTA avant / desk, 28-9888-89.
The illustration to the right refers to Tubing kit, AxiChrom 50 - 70 / ÄKTA avant / floor, 28-9888-92.
| Pos. |
28-9888-89 Tubing kit, AxiChrom 50 - 70 / ÄKTA avant / desk |
28-9888-92 Tubing kit, AxiChrom 50 - 70 / ÄKTA avant / floor |
|---|---|---|
| 1 | i.d. 1.7 / L=1200 (x2) | i.d. 1.7 / L=1600 (x2) |
| 2 | i.d. 1.7 / L=800 (x1) | i.d. 1.7 / L=1200 (x1) |
| 3 | i.d. 2.9 / L=1600 (x1) | i.d. 2.9 / L=1600 (x1) |
| 4 | i.d. 1.7 / L=200 (x2) | i.d. 1.7 / L=200 (x2) |
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| Tubing kit AxiChrom 50-70 AKTA avant, desk | 28988889 | 610.00 USD |
Add to cart
|
|
| Tubing kit AxiChrom 50-70 AKTA avant, floor | 28988892 | 618.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Conductivity Monitor C9 | 28956495 | 3,110.00 USD |
Add to cart
|
| Conductivity monitor (C9n) | 29011363 | 2,909.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| pH electrode | 29387193 | 828.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | UV flow cell, 0.5 mm, for U9-M | 28979386 | 3,667.00 USD |
Add to cart
|
| 2 | UV flow cell, 2 mm, for U9-M | 28979380 | 3,530.00 USD |
Add to cart
|
| 3 | UV flow cell, 10 mm, for U9-M | 28956378 | 2,375.00 USD |
Add to cart
|
| UV flow cell, 5 mm, for U9-L | 18112824 | 2,622.00 USD |
Add to cart
|
|
| UV flow cell, 2 mm, for U9-L | 29011325 | 2,835.00 USD |
Add to cart
|
|
| UV Monitor U9-L | 29011360 | 6,564.00 USD |
Add to cart
|
Spare parts
Seals are considered as consumables and should be replaced annually or when needed. Please keep a set of each in your stock to prevent long downtime. For code numbers refer to the table below.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | P9H Check valve kit | 28979364 | 1,141.00 USD |
Add to cart
|
| 2 | P9H Piston kit 150 ml | 28979368 | 912.00 USD |
Add to cart
|
| 3 | P9H Seal kit 150 ml | 28979373 | 729.00 USD |
Add to cart
|
| 4 | O-Ring, 1 × 3 mm, PFR | 19003601 | 89.07 USD |
Add to cart
|
| 5 | Inlet filter holder kit | 11000407 | 167.67 USD |
Add to cart
|
| 6 | Inlet filter set | 11000414 | 109.71 USD |
Add to cart
|
| 7 | Rinse system tubing | 28956504 | 127.30 USD |
Add to cart
|
| 8 | Flow Restrictor FR-902 | 18112135 | 758.00 USD |
Add to cart
|
| 10 | O-ring 5.3 x 2.4 mm | 18111860 | 76.73 USD |
Add to cart
|
| 11 | Mixer chamber 1.4 ml | 28956225 | 1,370.00 USD |
Add to cart
|
| 12 | Mixer chamber 5 ml | 28956246 | 1,370.00 USD |
Add to cart
|
| 13 | O-ring 13.1x1.6 mm | 28953545 | 86.95 USD |
Add to cart
|
| 14 | Mixer chamber 15 ml | 28980309 | 1,969.00 USD |
Add to cart
|
| 15 | O-ring 22.1 x 1.6 mm | 28981857 | 139.72 USD |
Add to cart
|
| 16 | Online filter kit | 18102711 | 144.90 USD |
Add to cart
|
| 17 | UV flow cell, 0.5 mm, for U9-M | 28979386 | 3,667.00 USD |
Add to cart
|
| 18 | UV flow cell, 2 mm, for U9-M | 28979380 | 3,530.00 USD |
Add to cart
|
| 19 | UV flow cell, 10 mm, for U9-M | 28956378 | 2,375.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Reference capillary 1 | 28950749 | 136.97 USD |
Add to cart
|
| 2 | Reference capillary 2 | 28950750 | 136.97 USD |
Add to cart
|
| 3 | Replacement tubing kit for ÄKTA avant 150 | 28979446 | 2,416.00 USD |
Add to cart
|
| 4 | Tubing kit for ÄKTA avant 150 | 28980987 | 318.00 USD |
Add to cart
|
| 5 | Complete tubing tag kit | 28956608 | 680.00 USD |
Add to cart
|
| 6 | Rinse system tubing | 28956504 | 127.30 USD |
Add to cart
|
| 7 | Union 1/16 male/male, i.d. 0.5 mm | 28954326 | 164.56 USD |
Add to cart
|
| 8 | Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 123.16 USD |
Add to cart
|
| 9 | Connector for 1/8" tubing | 18112117 | 199.76 USD |
Add to cart
|
| 10 | Ferrule for o.d. 1/8" Tubing | 18112118 | 145.94 USD |
Add to cart
|
| 11 | Stop Plug 1/16" Male | 18111252 | 59.00 USD |
Add to cart
|
| 12 | Ferrules for o.d. 1/16" tubing connector | 18112706 | 159.39 USD |
Add to cart
|
| 13 | Tubing kit for ÄKTA avant 25 | 28980984 | 306.00 USD |
Add to cart
|
| 14 | BufferPro inlet tubing kit | 28980998 | 135.58 USD |
Add to cart
|
| 15 | Inlet/Outlet Tubing Tag Kit for ÄKTA avant 150 | 28981004 | 255.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Inlet filter holder kit | 11000407 | 167.67 USD |
Add to cart
|
| 2 | Inlet filter set | 11000414 | 109.71 USD |
Add to cart
|
| 3 | Jumper 1 IEC 1394 | 28956489 | 53.82 USD |
Add to cart
|
| 4 | Cassette tray | 28954209 | 310.00 USD |
Add to cart
|
| 5 | Cassette for 50 ml tubes | 28956402 | 197.68 USD |
Add to cart
|
| 6 | Cassette, for 15 ml tubes | 28956404 | 197.68 USD |
Add to cart
|
| 7 | Cassette for fraction collection in 8 mL tubes | 28956425 | 198.72 USD |
Add to cart
|
| 8 | Cassette, for 3 ml tubes | 28956427 | 198.72 USD |
Add to cart
|
| 9 | Cassette for fraction collection in deep-well plate | 28954212 | 194.58 USD |
Add to cart
|
| 10 | Rack, for 50 ml tubes | 28980319 | 774.00 USD |
Add to cart
|
| 11 | Column Holder Rod | 28956270 | 1,017.00 USD |
Add to cart
|
| 12 | Online filter kit | 18102711 | 144.90 USD |
Add to cart
|
| 13 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 99.55 USD |
Add to cart
|
| 14 | Complete tubing tag kit | 28956608 | 680.00 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Mains cable 220 V | 19244801 | 110.80 USD |
Add to cart
|
| 2 | Mains cable, 120 V | 19244701 | 71.49 USD |
Add to cart
|
| 3 | Column Holder | 28956282 | 138.69 USD |
Add to cart
|
| 4 | Bottle holder | 28956327 | 306.00 USD |
Add to cart
|
| 5 | Tubing holder, comb | 28956286 | 83.84 USD |
Add to cart
|
| 6 | Tubing holder, spool, for small tubing | 28956274 | 83.84 USD |
Add to cart
|
| 7 | Tube holder | 28954329 | 52.78 USD |
Add to cart
|
| 8 | Inlet filter holder kit | 11000407 | 167.67 USD |
Add to cart
|
| 9 | Column Clamp | 28956319 | 83.84 USD |
Add to cart
|
| 10 | New Purge Kit P-900 | 18112453 | 91.08 USD |
Add to cart
|
| 11 | Online filter kit | 18102711 | 144.90 USD |
Add to cart
|
| 12 | Mixer chamber 1.4 ml | 28956225 | 1,370.00 USD |
Add to cart
|
| 13 | Mixer chamber 15 ml | 28980309 | 1,969.00 USD |
Add to cart
|
| 14 | Wrench, 1/4" | 18111245 | 111.78 USD |
Add to cart
|
| 15 | UniTag (sheet with 108 labels) | 28956491 | 96.26 USD |
Add to cart
|
| 16 | Screw lid kit | 11000410 | 49.68 USD |
Add to cart
|
| 17 | Stop Plug 1/16" Male | 18111252 | 59.00 USD |
Add to cart
|
| 18 | Fingertight Connector 1/16" Male for Tubing o.d. 1/16" | 18111255 | 123.16 USD |
Add to cart
|
| 19 | Fingertight connector 1/16" male, narrow | 28401081 | 108.65 USD |
Add to cart
|
| 20 | Connector 1/16" Male/Luer Female | 18111251 | 109.24 USD |
Add to cart
|
| 21 | Connector for 1/8" tubing | 18112117 | 199.76 USD |
Add to cart
|
| 22 | Ferrule for o.d. 1/8" Tubing | 18112118 | 145.94 USD |
Add to cart
|
| 23 | Connector 3/16" Male for Tubing o.d. 3/16" | 18111249 | 314.00 USD |
Add to cart
|
| 24 | Ferrules for 3/16" o.d. tubing connector | 18111248 | 130.53 USD |
Add to cart
|
| 25 | Connector 1/16" Male | 18112707 | 316.00 USD |
Add to cart
|
| 26 | Ferrules for o.d. 1/16" tubing connector | 18112706 | 159.39 USD |
Add to cart
|
| 27 | Reference capillary 1 | 28950749 | 136.97 USD |
Add to cart
|
| 28 | Tubing kit for ÄKTA avant 150 | 28980987 | 318.00 USD |
Add to cart
|
| 29 | Tubing kit for ÄKTA avant 25 | 28980984 | 306.00 USD |
Add to cart
|
| 30 | BufferPro inlet tubing kit | 28980998 | 135.58 USD |
Add to cart
|
| 31 | Inlet/Outlet Tubing Tag Kit for ÄKTA avant 150 | 28981004 | 255.00 USD |
Add to cart
|
| 32 | Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm | 18111246 | 99.55 USD |
Add to cart
|
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 1 | Flow Restrictor FR-902 | 18112135 | 758.00 USD |
Add to cart
|
| 3 | P9H Check valve kit | 28979364 | 1,141.00 USD |
Add to cart
|
| 3 | P9H Piston kit 150 ml | 28979368 | 912.00 USD |
Add to cart
|
| 3 | P9H Seal kit 150 ml | 28979373 | 729.00 USD |
Add to cart
|
| 4 | UV flow cell, 10 mm, for U9-M | 28956378 | 2,375.00 USD |
Add to cart
|
| 4 | UV flow cell, 2 mm, for U9-M | 28979380 | 3,530.00 USD |
Add to cart
|
| 4 | UV flow cell, 0.5 mm, for U9-M | 28979386 | 3,667.00 USD |
Add to cart
|
| 5 | Mixer chamber 1.4 ml | 28956225 | 1,370.00 USD |
Add to cart
|
| 5 | Mixer chamber 5 ml | 28956246 | 1,370.00 USD |
Add to cart
|
| 5 | Mixer chamber 15 ml | 28980309 | 1,969.00 USD |
Add to cart
|
| 6 | Conductivity Monitor C9 | 28956495 | 3,110.00 USD |
Add to cart
|
| 7 | Dummy Module | 28956493 | 1,226.00 USD |
Add to cart
|
| O-ring 22.1 x 1.6 mm | 28981857 | 139.72 USD |
Add to cart
|
Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Pressure monitors
Issues related to Pressure monitors
| Possible cause | Suggested remedy |
|---|---|
The cable between the Pressure monitors and the ICU is not connected. |
Remove the monitor and make sure that the cable is connected. |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
The monitors have lost their calibration. |
Calibrate the pressure monitors. See Section 7.6.2, in the User Manual. |
The temperature has changed. |
Wait until the temperature has stabilized and calibrate the pressure monitors. |
Incorrect installation of extra column valve. |
Check installation positions and tubing connections. |
| Possible cause | Suggested remedy |
|---|---|
Air bubbles are passing through or are trapped in the pump. |
Check that there is a sufficient volume of buffer present in the flasks. |
The check valve does not function correctly |
Remove any solids in the valves by cleaning the check valves according to the instructions in Section 7.5.7, in the User Manual. |
Piston seal is leaking |
Replace the piston seal according to the instructions in Sections 7.7.9, in the User Manual. |
Blockage of flow path |
Use the predefined method Prepare System to flush through to clear blockage. Check the column. It can be clogged if unfiltered buffers or samples are applied. To clean a column, see Section 7.5.3, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Dirty online filter in the mixer |
Change the online filter in the mixer. See Section 7.7.3, in the User Manual. |
Solids in the flow path |
To use the predefined method System CIP to clean the flow path, see Section 7.5.2, in the User Manual. To clean the column, see Section 7.5.3, in the User Manual. If persistent, replace the column. |
Column valve
Issues related to Column valve
| Possible cause | Suggested remedy |
|---|---|
Wrong Node ID. |
Check the module´s Node ID. If necessary, change the Node ID. See Section 9.3.4, in the User Manual |
The cable between the Column valve and the ICU is not connected. |
Remove the valve and make sure that the cable is connected. See Section 9.3.1, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Hardware error. |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. If this error is recurrent, generate a System error report and contact Service. To replace the valve, see Chapter 11, in the User Manual, for ordering information and Section 9.3.2, in the User Manual, for installation instructions. |
Hot surroundings. |
Decrease the room temperature. Maximum operating temperature is 35°C. |
The air intake is covered. |
Uncover the air intake. |
Outlet valve
Issues related to Outlet valve
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered. |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. To replace the valve, see Chapter 11, in the User Manual, for ordering information and Section 9.3.2, in the User Manual for installation instructions. |
| Possible cause | Suggested remedy |
|---|---|
The cable between the outlet valve and the ICU is not connected. |
Remove the valve and make sure that the cable is connected. See Section 9.3.1, in the User Manual. |
Wrong Node ID. |
Check the module´s Node ID. If necessary, change the Node ID. See Section 9.3.4, in the User Manual. |
Quarternary valve
Issues related to Quarternary valve
| Possible cause | Suggested remedy |
|---|---|
The sum of the flow from inlet Q1-Q4 is not equal to 100% of the total flow. |
In UNICORN, make sure that the sum of the flow from inlet Q1-Q4 is equal to 100% of the total flow. |
| Possible cause | Suggested remedy |
|---|---|
The switch valve is not opened and closed correctly |
Perform Performance test according to ÄKTAavant and UNICORN 6 Installation Guide. Check result and if necessary, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Hardware error |
Generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered. |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
Hardware error. |
Generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Broken valve. |
To replace the valve, contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Leaking tubing connectors. |
Check the tubing connectors. Tighten or replace the connectors. To order connectors, see Ordering information in Chapter 11, in the User Manual. |
Dummy modules
Issues related to Dummy modules
| Possible cause | Suggested remedy |
|---|---|
A dummy module is missing and the position is left empty. |
Assemble the missing dummy module, see Section 9.3.1, in the User Manual. |
General advice to achieve good performance
Before using the system make sure that:
- The correct system has been selected in UNICORN System Control
- The correct wavelength has been set for UV/UPC monitor
- All tubing has been properly connected
- All connectors are free from leakage
- No tubing is folded or twisted
- Online filter, if used, is changed on a regular basis
- Correct buffers are used for the chosen columns and proteins
- All inlet tubing has been immersed in correct buffer solutions
- Enough buffer has been prepared
- Buffers have been equilibrated to the environment temperature
- Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
- Suitable columns have been selected for the target proteins
- Columns have been cleaned and prepared according to column instructions
- Samples have been clarified by centrifugation and/or filtration prior to sample loading
- Samples have been adjusted to binding buffer conditions
- Auto sampler (if used) has been prepared according to user manual
- The fraction collector has been filled with appropriate number of microtiter plates or tubes
- Appropriate arrangement for waste handling has been prepared
pH monitor and pH valve
Issues related to pH monitor and pH valve
| Possible cause | Suggested remedy |
|---|---|
Calibration time out |
Check the connections between pH electrode and pH monitor. |
Bad pH electrode |
Regenerate the pH electrode. Place the electrode in deionized water for 30 minutes followed by 30 minutes in a buffer with pH 4. If persistent, replace the pH electrode. See Section 7.4.2, in the User Manual. |
Wrong mixer size for the used flow rate |
Use the recommended mixer size for the used flow rate. See Section 5.4.5, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
The cable between the pH valve and the ICU is not connected |
Remove the pH valve and make sure that the cable is connected. See Section 9.3.1, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
The temperature compensation of the pH monitor is turned off. |
Contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Waste tubing is twisted or blocked. |
Untwist the tubing. Change the tubing. |
| Possible cause | Suggested remedy |
|---|---|
Decreasing salt concentration in the electrode membrane due to osmosis to buffer |
Regenerate the pH electrode. Place the electrode in deionized water for 30 minutes followed by 30 minutes in a buffer with pH 4. |
UV monitor and UV detection unit
Issues related to UV monitor and UV detection unit
| Possible cause | Suggested remedy |
|---|---|
No UV flow cell is attached |
Attach UV cell. See Section 7.7.5, in the User Manual. |
UV flow cell is not correctly installed. |
Please verify that the UV cell is correctly installed. See Section 7.7.5, in the User Manual. |
The UV flow cell is broken |
Replace the cell. See Section 7.7.5, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Bad pump function |
Check that the pump is operating properly. See Section 8.5, in the User Manual for example of pump pressure curves. |
Poor mixing function |
Check the mixer chamber size and change the Check the function of the mixer. Place a stirrer bar in the palm of your hand. Hold the hand above the mixer. The stirrer should move when the mixer is activated. |
| Possible cause | Suggested remedy |
|---|---|
The detector is not correctly fitted |
Check that the detector is fitted correctly. See Section 7.7.5, in the User Manual If this error is recurrent, contact Service. |
Dirty optical fiber |
Clean the connectors. See Section 7.7.5, in the User Manual. |
Dirt on optical sensors in detector |
Remove visible dirt on detector photo diodes. |
Worn-out or broken lamp |
Contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Communication problem |
Contact Service |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
Wrong wavelength for current buffer |
Change wavelength or buffer. |
Dirt in the UV flow cell |
Clean the UV cell, see Section 7.4.1, in the User Manual. |
Dirty optical fiber connectors |
Clean the connectors. See Section 7.7.5, in the User Manual. |
Broken UV flow cell |
Replace UV flow cell. See Section 7.7.5, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Flow restrictor in off-line position |
Use the flow restrictor. Use the pH valve instruction to manually activate the flow restrictor (Flow path:pH valve:Restrictor in-line), or select the flow restrictor in the Method Settings phase of a method. |
Air in the UV flow cell |
Use the flow restrictor. |
Air in buffers |
De-gas if necessary. |
Impure buffers |
Check if the signal is noisy with water. |
Dirty optical fiber connectors |
Clean the connectors. See Section 7.7.5, in the User Manual. |
Dirt in the UV flow cell |
Perform a System CIP. See Section 7.5.2, in the User Manual. If necessary, manually clean the UV cell. See Section 7.4.1, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Wrong wavelength for current buffer |
Change wavelength or buffer. |
Dirty optical fiber connectors |
Clean the connectors. See Section 7.7.5, in the User Manual. |
Injection valve
Issues related to injection valve
| Possible cause | Suggested remedy |
|---|---|
Hardware error |
Generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered. |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. To replace the valve, see Chapter 11, in the User Manual, for ordering information and Section 9.3.2, in the User Manual for installation instructions. |
| Possible cause | Suggested remedy |
|---|---|
The cable between the Injection valve and the ICU is not connected. |
Remove the valve and make sure that the cable is connected. See Section 9.3.1, in the User Manual. |
Wrong Node ID. |
Check the module´s Node ID. If necessary, change the Node ID. See Section 9.3.4, in the User Manual. |
Conductivity monitor
Issues related to Conductivity monitor
| Possible cause | Suggested remedy |
|---|---|
Air in the conductivity flow cell. |
Use the flow restrictor.
Remove the air by flushing the flow cell with water or buffer. |
Leaking tubing connections |
Tighten the connectors. If necessary, replace the connectors. |
Dirty conductivity flow cell |
Clean the conductivity flow cell. See Section 7.5.8, in the User Manual |
Poor mixing function |
Check the mixer chamber size and change chamber if necessary. See Section 5.4.5, in the User Manual. Check the motor operation of the mixer. Place a magnet close to the mixer chamber during run. The magnet should be moving. Check that the mixer chamber is free from solids. To replace the online filter, see Section 7.7.3, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
The conductivity monitor needs to be calibrated |
Check calibration with a solution with known conductivity. Calibrate the conductivity monitor. See Section 7.6.3, in the User Manual. |
The ambient temperature may have decreased/increased |
Use a temperature compensation factor. The temperature compensation factor is found in System Control:System Settings:Conductivity. Instruction regarding the factor is also found in Section 7.6.3, in the User Manual. |
Dirty conductivity flow cell |
Clean the conductivity cell. See Section 7.5.8, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Air bubbles are passing through the flow cell |
Check for loose tubing connections. |
A charged particle has been detected |
Prepare the sample so that charged particles are eliminated. |
| Possible cause | Suggested remedy |
|---|---|
Poor mixing function |
Check that the correct mixer chamber size is used. See Section 5.4.5, in the User Manual, for recommendations. To change the mixer, see Section 7.7.2, in the User Manual. Check the motor operation of the mixer. Place a magnet close to the mixer chamber during run. The magnet should be moving. Check that the mixer chamber is free from solids. To open the mixer, see Section 7.7.3, in the User Manual. |
The column is not equilibrated |
Equilibrate the column. Use the method phase Equilibration. |
The temperature compensation factor is not |
Use a temperature compensation factor. The temperature compensation factor is found in System Control:System Settings:Conductivity. Instruction regarding the factor is also found in Section 7.6.3, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
The mixer chamber is too large |
Change to a mixer chamber with a smaller volume. See Section 5.4.5, in the User Manual, for recommendations. To change the mixer, see Section 7.7.2, in the User Manual. |
Bad pump function |
Make sure that the pump operates properly. See Section 8.5, in the User Manual for example of pump pressure curves. |
Make sure that the tubing has been washed properly. |
Dirt in the tubing |
| Possible cause | Suggested remedy |
|---|---|
This error could only occur when the temperature compensation is turned on. The error will occur when the temperature is outside the range 2°C to 40°C. |
Make sure the temperature of the calibration solution is within 2°C and 40°C. |
| Possible cause | Suggested remedy |
|---|---|
Internal errors |
See error log. Restart instrument and retry. If this problem recurs, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Solids in the conductivity flow cell |
Clean the conductivity cell. See Section 7.5.8, in the User Manual. |
Air in the conductivity flow cell |
Flush the conductivity flow cell with water. |
| Possible cause | Suggested remedy |
|---|---|
Bad pump function |
Check that the pump is operating properly. See Section 8.5, in the User Manual, for example of pump pressure curves. |
Air in the flow path |
Purge the pumps. See Section 5.5.2, in the User Manual and Section 5.5.3, in the User Manual. |
Poor mixing function |
Check that the correct mixer chamber size is used. See Section 5.4.5, in the User Manual, for recommendations. To change the mixer, see Section 7.7.2, in the User Manual. Check the motor operation of the mixer. Place a magnet close to the mixer chamber during run. The magnet should be moving. Check that the mixer chamber is free from solids. To open the mixer, see Section 7.7.3, in the User Manual. |
Inlet valves
Issues related to inlet valves
The inlet valves include Inlet valve A, Inlet valve B and sample inlet valve.
| Possible cause | Suggested remedy |
|---|---|
Hardware error |
Generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
The air intake is covered. |
Uncover the air intake. |
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
Wrong Node ID |
Check the module´s Node ID. If necessary, change the Node ID. See Section 9.3.4, in the User Manual. |
The cable between the inlet valve and the ICU is not connected |
Remove the valve and make sure that the cable is connected. See Section 9.3.1, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Broken valve. |
Replace the valve. |
| Possible cause | Suggested remedy |
|---|---|
Hardware error |
Restart the instrument with the power switch. If this error is recurrent, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Leaking tubing connectors. |
Check the tubing connectors. Tighten or replace the connectors. To order connectors, see Ordering information in Chapter 11, in the User Manual. |
Fraction collector
Issues related to Fraction collector
| Possible cause | Suggested remedy |
|---|---|
Too many commands are pending in the fraction collector |
The reason could be that too many fraction collector instructions have been sent. Wait for a while and try again. |
| Possible cause | Suggested remedy |
|---|---|
The cassette tray positioning discs in the frac chamber are missing |
Replace the cassette tray positioning discs in the frac chamber. See Section 7.5.4, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Hot surroundings |
Decrease the room temperature. Maximum operating temperature is 35°C. |
Hardware error |
Switch off the instrument and wait until the temperature has decreased. Restart the instrument. |
| Possible cause | Suggested remedy |
|---|---|
Dirty cassette type code reader |
Clean the dispenser head and its four diode windows using a cloth and a mild cleaning agent or 20% ethanol. See Section 7.5.4, in the User Manual, for more information. |
Dirty cassette type codes |
Clean the cassette type codes. See Section 7.5.4, in the User Manual, for more information. |
| Possible cause | Suggested remedy |
|---|---|
Air in the flow path |
Check the flow path for air. Fill system and purge pumps according to Section 5.5, in the User Manual. |
Dirty drop sync sensor diode windows |
Clean the drop sync sensor diode windows. See Section 7.5.4, in the User Manual. If this error is recurrent, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Too high flow rate |
Decrease the flow rate. |
Air in the flow path |
Check the flow path for air. Fill system and purge pumps according to Section 5.5, in the User Manual. |
Dirty drop sync sensor diode windows |
Clean the drop sync sensor diode windows. See Section 7.5.4, in the User Manual. |
| Possible cause | Suggested remedy |
|---|---|
Air in the flow path |
Check the flow path for air. Fill system and purge pumps according to Section 5.5, in the User Manual. |
Dirty drop sync sensor diode windows |
Clean the drop sync sensor diode windows. See Section 7.5.4, in the User Manual. If this error is recurrent, generate a System error report and contact Service. |
Too high flow rate |
Decrease the flow rate. |
| Possible cause | Suggested remedy |
|---|---|
The cable between the fraction collector and the ICU is not connected |
Generate a System error report and contact Service. |
A fuse in the instrument ICU is broken |
The ICU needs to be changed. Generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Salt crystals or protein residuals block the accumulator. |
Restart the instrument and perform an accumulator wash. |
Mechanical error |
If this error is recurrent, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Obstruction inside the fraction collector. |
Switch off the instrument and check for obstruction inside the fraction collector. Try to move the Frac arm by hand. Switch on the instrument. If this error is recurrent, generate a System error report and contact Service. |
| Possible cause | Suggested remedy |
|---|---|
Dirty drop sync sensor diode windows |
Clean the drop sync sensor diode windows. See Section 7.5.4, in the User Manual, for location of the drop sync sensor diode windows and cleaning instruction. If this error is recurrent, generate a System error report and contact Service. |
Maintenance
Maintenance instructions and procedures.
ÄKTA avant
Periodic maintenance program
This section lists the periodic maintenance activities that should be performed by the user of ÄKTA avant, as well as maintenance activities that should be performed when required.
Hazardous biological agents during run. When using hazardous biological agents, run System CIP and Column CIP to flush the entire system tubing with bacteriostatic solution (e.g., NaOH) followed by a neutral buffer and finally distilled water, before service and maintenance.
Hazardous chemicals during run. When using hazardous chemicals, run System CIP and Column CIP to flush the entire system tubing with distilled water, before service and maintenance.
Always use appropriate personal protective equipment during operation and maintenance.
Maintenance operations should be performed by the user at regular intervals
The following maintenance should be performed by the user of ÄKTA avant daily.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Calibrate the pH monitor | Section 7.7.1 Calibrate the pH monitor. |
The following maintenance should be performed by the user of ÄKTA avant weekly.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Calibrate pressure monitors | Section 7.7.2 Calibrate the pressure monitors. |
Change pump piston rinsing solution
The following maintenance should be performed by the user of ÄKTA avant weekly.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Change pump rinsing solution | Section 7.3.1 Change pump rinsing solution. |
The following maintenance should be performed by the user of ÄKTA avant monthly.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Check the flow restrictor | Section 7.4.1 Check the flow restrictor. |
The following maintenance should be performed by the user of ÄKTA avant every 6 months.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Clean the UV flow cell | Section 7.5.1 Clean the UV flow cell. |
| Replace pH electrode | Section 7.5.2 Replace the pH electrode. |
The following maintenance should be performed by the user of ÄKTA avant when required.
The detailed procedures can be found in the ÄKTA avant User Manual located in the related documents tab.
| Maintenance | See action |
| Clean the instrument externally | Section 7.6.1 Clean the instrument externally. |
| Perform System CIP | Section 7.6.2 Perform System CIP. |
| Perform Column CIP | Section 7.6.3 Perform Column CIP. |
| Clean the Fraction collector | Section 7.6.4 Clean the Fraction collector. |
| Replace tubing and connectors | Section 7.8.1 Replace tubing and connectors. |
| Storage of pH electrode | Section 7.6.5 Storage of the pH electrode. |
| Clean the pH electrode | Section 7.6.6 Clean the pH electrode. |
| Clean the conductivity flow cell | Section 7.6.8 Clean the conductivity flow cell. |
| Calibrate the Conductivity monitor | Section 7.7.3 Calibrate the Conductivity monitor. |
| Calibrate the UV monitor | Section 7.7.4 Calibrate the UV monitor. |
| Replace mixer | Section 7.8.2 Replace the mixer. |
| Replace inline filter | Section 7.3.2 Replace the inline filter. |
| Replace O-ring in mixer | Section 7.8.3 Replace the O-ring of the mixer. |
| Replace UV flow cell | Section 7.8.4 Replace the UV flow cell. |
| Replace flow restrictor | Section 7.8.5 Replace the flow restrictor. |
| Replace inlet filters | Section 7.8.6 Replace inlet filters. |
| Clean the check valves | Section 7.6.7 Clean the pump head check valves. |
| Replace check valves | Section 7.8.7 Replace the pump head check valves. |
| Replace pump piston seals | Section 7.8.8 Replace pump piston seals of Pump P9 or P9H. and Section 7.8.9 Replace pump piston seals of pump P9-S. |
| Replace pump pistons | Section 7.8.10 Replace pump pistons. |
| Replace pump rinsing system tubing | Section 7.8.11 Replace pump rinsing system tubing. |
| Replace valve modules | Section 7.8.12 Replace a valve module. |