Cellbag BioClear 10, BC10, pHOPT, DO
Presterilized, single-use bioreactor bags for non-invasive cell cultivation, designed for use with WAVE™ Bioreactor systems.
Accessories
| Port No. | Description | Associated products |
|---|---|---|
| 1 | 1/4 in × 7/16 in × 39 in C-Flex®, MPC female | Connector MPC male and tubing i.d. 1/4 in |
| 2 | 1/8 in × 1/4 in × 39 in C-Flex®, Luer female | Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001) |
| 3 | 3/16 in × 3/8 in × 2 in silicone, needleless sampling | Syringe with Luer male connection |
| 4 | 3/16 in × 3/8 in × 2 in silicone, Luer female | Connector Luer male and tubing i.d. 3/16 in |
| 5 | Tempwell for RTD probe | RTD temperature probe 28411667 |
| 6 | Air outlet filter, check valve |
Electrical heater for cellbag vent filter 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system |
| 7 | Air inlet filter | Tubing to connect inlet filter to system supplied with system |
| 8 | Oxywell2, for DOOPT probe | DOOPT-Probe 28411672 and Oxywell2 28412451 |
| 9 | Optical pH sensor embedded in bag | Bag sensor adaptor 28984189 |
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 6 | Electric Exhaust Filter Heater | 28411639 | 548.00 USD |
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Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Troubleshooting for Cellbags
| Possible cause | Suggested remedy |
|---|---|
Too high airflow |
Check that the airflow to the bioreactor does not exceed 0.5 Lpm. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.) |
Faulty relief valve |
Check that air is passing out of the pressure relief valve: attach a short length of tubing to the exhaust vent and immerse the tubing in water to a 1 cm depth. Bubbles should appear indicating flow. If no flow is observed, remove the pressure relief valve. The outlet filter may be plugged and removing the pressure relief valve may allow continued operation. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.) |
Faulty Cellbag |
If the Cellbag continues to overinflate, transfer the contents to another Cellbag. (The Cellbag should be inflated so that it requires force to squeeze it. However, it should not be so pressurized that creases form near the attachment points.)
|
| Possible cause | Suggested remedy |
|---|---|
Too low airflow |
Check that there is sufficient air flow to the inlet filter.
(An underinflated Cellbag will generate excessive foam and poor mixing.)
|
Inlet air supply wrongly connected |
Check that you have connected the inlet air supply line to the inlet filter. |
Missing pressure relief valve |
Check that the pressure relief valve is present on the exhaust filter. |
Obstructed flow paths |
Check that air inlet and out flow paths are unobstructed. |
Clogged exhaust filter |
If the exhaust filter is clogged then transfer the contents to another Cellbag. |
| Possible cause | Suggested remedy |
|---|---|
Poorly inflated Cellbag |
Check that the Cellbag is rigidly inflated. A poorly inflated bag will foam rapidly. |
Too high rocking rate |
If foam is still excessive after a few hours of operation, reduce the rocking rate. Make sure that the new rocking rate provides a sufficient dissolved oxygen concentration. |
Too big rock angle |
Reduce the rock angle. The bioreactor base units are factory set to ±6° from the horizontal. This angle has been found optimal for most cell lines. |