Cellbag BioClear 10, DOOPT II and pHOPT

ID	Product Name
28412411	Cellbag BioClear 10
28412278	Cellbag BioClear 10
28412286	Cellbag BioClear 10
28412304	Cellbag BioClear 10
28412268	Cellbag BioClear 10
28412269	Cellbag BioClear 10
28412305	Cellbag BioClear 10 (Oxywell Version)
28412399	Cellbag BioClear 10 (Oxywell Version)
28412287	Cellbag BioClear 10 (Oxywell Version)
28412279	Cellbag BioClear 10 (Oxywell Version)
28412301	Cellbag BioClear 10 (Oxywell Version)
28412270	Cellbag BioClear 10 (Oxywell Version)
28412342	Cellbag BioClear 10 (Perfusion Version)
28412359	Cellbag BioClear 10 (Perfusion Version)
28412354	Cellbag BioClear 10 (Perfusion Version)
29176218	Cellbag BioClear 10 (Perfusion Version)
28412280	Cellbag BioClear 10 (Screw Cap Port)
28412288	Cellbag BioClear 10 (Screw Cap Port)
28412271	Cellbag BioClear 10 (Screw Cap Port)
28412397	Cellbag BioClear 10, (Oxywell Version)
28413020	Cellbag BioClear 10, BC10, DO, RM
28413019	Cellbag BioClear 10, BC10, DO, RM
28413018	Cellbag BioClear 10, BC10, DO, RM
28413017	Cellbag BioClear 10, BC10, DO, RM
28413016	Cellbag BioClear 10, BC10, DO, RM
28984080	Cellbag BioClear 10, BC10, pHOPT, DO
28984089	Cellbag BioClear 10, BC10, pHOPT, DO
28984079	Cellbag BioClear 10, BC10, pHOPT, DO
28984074	Cellbag BioClear 10, BC10, pHOPT, DO
28984090	Cellbag BioClear 10, BC10, pHOPT, DO
28984092	Cellbag BioClear 10, BC10, pHOPT, DO
28984091	Cellbag BioClear 10, BC10, pHOPT, DO
29018907	Cellbag BioClear 10, DOOPT II and pHOPT
29015212	Cellbag BioClear 10, DOOPT II and pHOPT
29015216	Cellbag BioClear 10, DOOPT II and pHOPT
29015214	Cellbag BioClear 10, DOOPT II and pHOPT
29015215	Cellbag BioClear 10, DOOPT II and pHOPT
29018906	Cellbag BioClear 10, DOOPT II and pHOPT
29015312	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015213	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015315	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015308	Cellbag BioClear 10, DOOPT II, pHOPT and perfusion
29015444	Cellbag BioClear 10, DOOPT II, pHOPT and Perfusion (Thick Filter)
29015309	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29015307	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29015313	Cellbag BioClear 10, DOOPT II, pHOPT and screwcap
29012355	Cellbag BioClear 10, pHOPT and perfusion
29012460	Cellbag BioClear 10, pHOPT and perfusion
29012360	Cellbag BioClear 10, pHOPT and perfusion
29012470	Cellbag BioClear 10, pHOPT and Perfusion (Standard Filter)
29012357	Cellbag BioClear 10, pHOPT and screwcap
29012353	Cellbag BioClear 10, pHOPT and screwcap

Troubleshooting
Accessories

Accessories

Accessories for Cellbag DOOPT II and pHOPT version CB0100L10-31

Port No.	Description	Associated products
1	3/8 in × 5/8 in × 78 in C-Flex, ReadyMate	ReadyMate DAC 250 with 1/4 in barb 28936691 (50 pieces) and 28936612 (10 pieces) ReadyMate DAC 375 with 3/8 in barb 28936692 (50 pieces) and 28936687 (10 pieces) ReadyMate DAC 500 with 1/2 in barb 28936693 (50 pieces) and 28936688 (10 pieces) ReadyMate DAC 750 with 3/4 in barb 28936694 (50 pieces) and 28936689 (10 pieces) ReadyMate DAC 750 Mini TC 28936707 (50 pieces) and 28936695 (10 pieces) ReadyMate DAC 1500 TC 28956890 (50 pieces) and 28956889 (10 pieces) ReadyClamp 28936690 (25 pieces)
2	3/8 in × 5/18 in × 78 in C-Flex®, MPC male	Connector MPC female and tubing i.d. 3/8 in
3	1/8 in × 1/4 in × 2 in C-Flex® Y-connector	Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001)
4	3/16 in × 3/8 in × 2 in silicone, needleless sampling	Syringe with Luer male connnection
5	1/4 in × 7/16 in × 78 in C-Flex®, MPC male	Connector MPC female and tubing i.d.1/4 in
6	1/8 in × 1/4 in × 78 in C-Flex®, Luer female	Clave sampling valve on 6.4 mm o.d. C-Flex® tubing 28412412 (TK001)
7	Tempwell for RTD probe	RTD temperature probe 28411667
8	Air inlet filter	Tubing to connect inlet filter to system supplied with system
9	Air outlet filter, check valve	Electrical heaters for cellbag vent filters 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system
10	Air outlet filter, check valve	Electrical heater for cellbag vent filter 28411639, check valve WV050087 Tubing to connect outlet filter to system supplied with system
11	3/8 in × 5/18 in × 78 in C-Flex®, MPC male, silicon diptube	Connector MPC female and tubing i.d. 3/8 in
12	Optical pH sensor embedded in bag	Bag sensor adaptor 28984189
13	Optical DO sensor embedded in bag	Bag sensor adaptor 28984189

Something went wrong - we were not able to load your agreed MSA pricing. Please try refreshing the page.

#	Product Name	Product Code	Price
1	ReadyMate Disposable Aseptic Connector (DAC) 250 with 1/4" Barb, 10	28936612	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 375 with 3/8" Barb, 10	28936687	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 500 with 1/2" Barb, 10	28936688	496.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 with 3/4" Barb, 10	28936689	495.00 USD	Add to cart
1	ReadyClamp	28936690	574.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 250 with 1/4" Barb, 50	28936691	2,038.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 375 with 3/8" Barb, 50	28936692	2,059.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 500 with 1/2" Barb, 50	28936693	2,059.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 with 3/4" Barb, 50	28936694	2,041.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 Mini TC, 10	28936695	547.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 750 Mini TC, 50	28936707	2,254.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 1500 TC, 10	28956889	593.00 USD	Add to cart
1	ReadyMate Disposable Aseptic Connector (DAC) 1500 TC, 50	28956890	2,494.00 USD	Add to cart
9	Electric Exhaust Filter Heater	28411639	548.00 USD	Add to cart
10	Electric Exhaust Filter Heater	28411639	548.00 USD	Add to cart
12	Bag Sensor Adaptor 2.5m Assy	28984189	1,289.00 USD	Add to cart
13	Bag Sensor Adaptor 2.5m Assy	28984189	1,289.00 USD	Add to cart

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Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Troubleshooting for Wave Bioreactor System 200 and Wave Bioreactor System 500

The Cellbag appears to be overinflated

Possible cause	Suggested remedy
The outlet filter is closed off or blocked.	Make sure that the outlet filter is not closed off or blocked. Try opening the spare (middle) filter to depressurize the Cellbag. You can connect this filter to the Exhaust pump and continue operations. If foam is clogging the outlet filter, reduce the rocking speed or the rock angle. (The Cellbag should be inflated so that it requires force to squeeze it 2 inches H₂O ± 0.2 inches for WAVE Bioreactor 200 and 3 inches H₂O ± 0.2 inches for WAVE Bioreactor 500/1000). However, it should not be so pressurized that creases form near the attachment points. The inlet airflow will shut off automatically (PV=0) if the pressure exceeds pressure setpoint +1 inch H₂O gauge)
If your are using an Exhaust pump - the Exhaust pump is disconnected or turned off	Make sure that the correct Exhaust pump is connected to the respective outlet filter. Check to see that the Exhaust pump is running. (The Cellbag should be inflated so that it requires force to squeeze it 2 inches H₂O ± 0.2 inches for WAVE Bioreactor 200 and 3 inches H₂O ± 0.2 inches for WAVE Bioreactor 500/1000). However, it should not be so pressurized that creases form near the attachment points. The inlet airflow will shut off automatically (PV=0) if the pressure exceeds pressure setpoint +1 inch H₂O gauge

Possible cause

Suggested remedy

The outlet filter is closed off or blocked.

Make sure that the outlet filter is not closed off or blocked. Try opening the spare (middle) filter to depressurize the Cellbag. You can connect this filter to the Exhaust pump and continue operations. If foam is clogging the outlet filter, reduce the rocking speed or the rock angle.

(The Cellbag should be inflated so that it requires force to squeeze it 2 inches H₂O ± 0.2 inches for WAVE Bioreactor 200 and 3 inches H₂O ± 0.2 inches for WAVE Bioreactor 500/1000). However, it should not be so pressurized that creases form near the attachment points. The inlet airflow will shut off automatically (PV=0) if the pressure exceeds pressure setpoint +1 inch H₂O gauge)

If your are using an Exhaust pump - the Exhaust pump is disconnected or turned off

Make sure that the correct Exhaust pump is connected to the respective outlet filter. Check to see that the Exhaust pump is running.

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Cellbag appears to be underinflated.

Possible cause	Suggested remedy
Too low airflow	Check that there is sufficient air flow to the inlet filter. (An underinflated Cellbag will generate excessive foam and poor mixing. NOTICE! Rocking an underinflated Cellbag will cause rapid failure of the Cellbag and possible leakage. Do not rock the unit if you are getting a low bag pressure alarm (< 0.2 inches H₂O).)
Clogged inlet filter	If the inlet filter is clogged, use the spare filter as an inlet filter. (An underinflated Cellbag will generate excessive foam and poor mixing. NOTICE! Rocking an underinflated Cellbag will cause rapid failure of the Cellbag and possible leakage. Do not rock the unit if you are getting a low bag pressure alarm (< 0.2 inches H₂O).)

Possible cause

Suggested remedy

Too low airflow

Check that there is sufficient air flow to the inlet filter.

(An underinflated Cellbag will generate excessive foam and poor mixing.
NOTICE! Rocking an underinflated Cellbag will cause rapid failure of the Cellbag and possible leakage. Do not rock the unit if you are getting a low bag pressure alarm (< 0.2 inches H₂O).)

Clogged inlet filter

If the inlet filter is clogged, use the spare filter as an inlet filter.

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Excessive foaming in the Cellbag

Possible cause	Suggested remedy
Poorly inflated Cellbag	Check that the Cellbag is rigidly inflated. A poorly inflated Cellbag will foam rapidly. (Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)
Too high rocking rate	In many cases foam will subside after a few hours of operation. If foam is still excessive, reduce the rocking rate. Check that this rocking rate still provides a sufficient dissolved oxygen concentration. (Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)
Too big rock angle	Reduce the rock angle. Reducing the angle will reduce foam while being able to maintain a higher rock rate. The WAVE Bioreactor base units are factory set to ±5° from the horizontal. This angle has been found to be optimal for most cell lines. (Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)

Possible cause

Suggested remedy

Poorly inflated Cellbag

Check that the Cellbag is rigidly inflated. A poorly inflated Cellbag will foam rapidly.

(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)

Too high rocking rate

In many cases foam will subside after a few hours of operation. If foam is still excessive, reduce the rocking rate. Check that this rocking rate still provides a sufficient dissolved oxygen concentration.

(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)

Too big rock angle

Reduce the rock angle. Reducing the angle will reduce foam while being able to maintain a higher rock rate. The WAVE Bioreactor base units are factory set to ±5° from the horizontal. This angle has been found to be optimal for most cell lines.

(Some amount of foam is typical. However, foam should not cover more than 50% of the surface area.)

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