Consumables

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# Product Name Product Code Price
Gel Filtration LMW Calibration Kit 28403841 835.37 USD Add to cart
Gel Filtration HMW Calibration Kit 28403842 835.37 USD Add to cart

Accessories

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# Product Name Product Code Price
Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 123.16 USD Add to cart
Filter Tool 18115320 166.05 USD Add to cart
Tricorn Storage/Shipping Device 18117643 139.40 USD Add to cart
Tricorn 10 Filter Kit 29053612 194.75 USD Add to cart

Running Prepacked Tricorn columns with ÄKTAexplorer, ÄKTAFPLC, ÄKTAprime plus, ÄKTApurifier and ÄKTAxpress.

 

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

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# Product Name Product Code Price
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 99.55 USD Add to cart
1 PEEK Tubing, 2 m, i.d. 0.75 mm, o.d. 1/16" 18111253 96.26 USD Add to cart
1 PEEK Tubing, 2 m, i.d. 0.5 mm, o.d. 1/16" 18111368 73.48 USD Add to cart
1 Tubing i.d. 0.25 mm, o.d. 1/16" 18112095 66.24 USD Add to cart
2 Fingertight Connector 1/16" Male for Tubing o.d. 1/16" 18111255 123.16 USD Add to cart

Running Prepacked Tricorn with ÄKTAmicro.

 

Make sure you have all connectors and tubing needed for running the column.

Depending on your system configuration and column length you may need extra long tubing to connect your column.

Please do not extend the tubing unnecessarily which results in a lower resolution.

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# Product Name Product Code Price
1 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 99.55 USD Add to cart
1 PEEK Tubing, 2 m, i.d. 0.15 mm, o.d.1/16" 18115659 446.00 USD Add to cart
2 Fingertight Narrow Head Connector, o.d. 1/16" 18114710 538.00 USD Add to cart

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# Product Name Product Code Price
1 TUBING FLANG 1,2X420 FINELINE 18110220 75.53 USD Add to cart
1 Outlet tubing P6000 18454601 58.06 USD Add to cart
1 Unflanged tubing i.d. 1.2 mm, o.d. 1.8 mm 19437002 622.00 USD Add to cart
3 Tubing cutter, for PEEK, EFTE, and FEP tubing i.d. 0.25, 0.5, 0.75, 1 and 1.6 mm 18111246 99.55 USD Add to cart
3 Tubing, 2 m i.d. 0.8 mm, o.d. 1.8 mm, ETFE 19743501 74.52 USD Add to cart
4 Pre-filter 6000 18458201 1,367.00 USD Add to cart
4 Filter kit 18458401 156.83 USD Add to cart
4 Holder Valve PSV-50 19199255 153.64 USD Add to cart
4 Solenoid Valve PSV-50 19199401 819.00 USD Add to cart
7 Fingertight Union 1/16" Male/M6 Female 18111258 135.19 USD Add to cart
8 UV Flow Cell, 2 mm 18111110 2,857.00 USD Add to cart
8 Fiber Detachment Tool 18111116 131.81 USD Add to cart
9 Filter 215 nm 11000733 808.00 USD Add to cart
9 Filter 260 nm 11000734 781.00 USD Add to cart
9 Filter 280 nm 11000735 628.00 USD Add to cart
9 Filter 405 nm 11000736 760.00 USD Add to cart
9 Empty Filter Holder 11000738 248.00 USD Add to cart
9 Monitor UVis-920 Excluding Filter and Flow Cell 11000754 10,200.00 USD Add to cart
9 Short optical fibre kit, 200 mm 18113485 970.00 USD Add to cart
9 Long optical fibre kit, 500 mm 18113486 716.00 USD Add to cart
9 UV flow cell, i.d. 8 mm 28966687 3,723.00 USD Add to cart
9 O-ring kit 28969705 162.00 USD Add to cart

Spare parts

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# Product Name Product Code Price
1 Filter Tool 18115320 166.05 USD Add to cart
1 Tricorn 10 Filter Kit 29053612 194.75 USD Add to cart

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

General advice to achieve good performance

Before using the column make sure that:

  • Correct system has been selected in UNICORN System Control
  • Correct wavelength has been set for UV/UPC monitor
  • All tubing has been properly connected and tubing is not longer than needed
  • All connectors are free from leakage, verified by passing a leakage test
  • No tubing is folded or twisted
  • Online filter, if used, is changed on a regular basis
  • Correct buffers are used for the chosen columns and proteins
  • All inlet tubing has been immersed in correct buffer solutions
  • Enough buffer has been prepared
  • Buffers have been equilibrated to the environment temperature
  • Buffers/eluents have been degassed if necessary (e.g., in RPC runs)
  • Suitable columns have been selected for the target proteins
  • Column meets pressure requirements for selected medium
  • Columns have been cleaned and prepared according to column instructions
  • Samples have been clarified by centrifugation and/or filtration prior to sample loading
  • Samples have been adjusted to binding buffer conditions
  • Auto sampler (if used) has been prepared according to user manual
  • The fraction collector has been filled with appropriate number of microtiter plates or tubes
  • Appropriate arrangement for waste handling has been prepared

 

 

Superdex 75, Superdex 200 Increase and Superose 6 Increase

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