Superose™ 6 Increase 3.2/300

-

Confirm that the column is the cause (see below). If so, clean it according to the procedure described in section Cleaning-in-place (CIP) in the Instructions.

To confirm that the high back-pressure in the system is caused by the column, disconnect one piece of equipment at a time (starting at the fraction collector) with the pumps running. Check the pressure reading after each piece has been disconnected to determine the source of the back-pressure.

-

Note that small amounts of air will normally not affect the performance of the column. Run 3 to 4 CV well de-gassed eluent in an upflow direction at a flow rate of 0.04 ml/min at room temperature.

-

Carefully tighten the black end cap in both ends of the column by hand.

-

Do not use the column in a system if the black end caps are loose. This is because the liquid could leak into the space between the inner column and the outer shell. Carefully tighten the black end caps by hand.