ReadyToProcess Capto Phenyl ImpRes 2.5 L (126/200)
These ReadyToProcess chromatography columns are prepacked with Capto Phenyl ImpRes chromatography resin and are excellent for intermediate and polishing in downstream protein purification. ReadyToProcess columns are validated high-performance bioprocessing columns that are supplied prepacked and ready for use.
FAQ
Same procedures as for packed conventional process columns. Equilibrate and store packed columns according to the instructions for the medium in the ReadyToProcess column user manual.
Same procedures as for packed conventional process columns. Please read the Application note: “Efficiency test of ReadyToProcess columns”, available in the Related Literature tab.
Product documentation—a user manual, including a certificate of conformance, is provided. The certificate of conformance shows conformance with 21CFR177, USP Class VI, and animal-free origin (or EMEA/410/01) for each wetted part material as well as traceability of the material. The product documentation contains a certificate of analysis showing packing performance as well as endotoxin and microbiology test results for the delivered column. The user manual is distributed with each column and the CoA can be downloaded from gelifesciences.com/certificates.
Validation guides—gives access to product information of the ReadyToProcess column including stability, validation, and quality as well as a brief description of the production process.
RSF—current files include additional information on each BioProcess chromatography resin. RSF addenda and RSF are available at gelifesciences.com/rsf
Rigorous transport studies have shown that there is no change in column performance after shipping. Beta testers confirm splendid performance. Shipping and drop tests are included in Regulatory Support File.
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Possible causes |
Suggested remedy |
|---|---|
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Column is clogged. |
1. Perform regeneration of the column |
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Bent tubing. |
Check that the flow path is not restricted. |
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Buffer viscosity too high. |
Check the viscosity of all buffers. Viscosity is a function of temperature. (Lower temperature gives higher viscosity.) Let low-temperature buffer reach operating temperature before starting the run. |
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Microbial growth in buffers. The buffer normally become opalescent due to microbial growth. |
Check buffers, especially those with phosphate, for microbial growth. Replace with fresh buffer if necessary. |
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Sample and collection vessels at different levels. |
Adjust the vessels to approximately the same level. |
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The prefilter might be blocked. |
Check the prefilter. |
| 1 L column | 2.5 L column | 5 L column | 10 L column | 20 L column | 32 L column |
|---|---|---|---|---|---|
| ~3 kg | ~6 kg | ~13 kg | ~25 kg | ~55 kg | ~96 kg* |
*Wheels for convenient handling.
Accessories
To isolate the column from the system use TC End Cap, 25 mm TC incl. gaskets 18-1001-25 and TC Clamp 25 mm.
For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82.
For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.
The ReadyToProcess 32 L (450/200) columns have an extra tube connected to the top outlet. This tube should be used to connect the column to the ÄKTA ready System.
| # | Product Name | Product Code | Price | |
|---|---|---|---|---|
| 5 | TC-Gasket 25 mm i.d. 15 mm | 44549288 | 209.00 USD |
Add to cart
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| 6 | TC clamp kit, 25 mm TC | 28404336 | 302.00 USD |
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| 7 | Blind Flange, including gasket, 25 mm | 18100125 | 82.80 USD |
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