FAQ

Accessories

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When running FlexStand Holder with peristaltic pump, the maximum pressure that can be achieved is 2 bar g (30 psi g).

The use of a permeate flow control is strongly recommended during upstream clarification of target material. 

A permeate flow control kit together with a pump for the flow rate is required for permeate flow control. Permeate flow control rate is dependent on the process flux (LMH) and surface area. 

Detailed information about Permeate control valve (56-4105-89) and Permeate flow control kit (56-4107-73), is available below.

Suitable peristaltic pump (56-4106-53) for permeate flow control, flow rates 5.4 ml/min - 2 liter/min. Information available below.

Countries using 220 V power, have to order power cords separately for the pump above and system.

Feed pump (11-0005-66 or 11-0005-67), Feed reservoir (56-4107-67 or 56-4107-70), Outlet pressure gauge for FS-03LVS (56-4106-04), Outlet pressure gauge for FS-03LVS-30 ( 56-4106-03) and Tubing (56-4106-19, 56-4106-22 or 56-4105-23) have to be ordered separately.

After use, store the cartridge with liquid inside using blank off cap, gasket and clamp on the feed and retentate ports.

Seal the permeate port with a short piece of tubing i.d. 9.5 mm, which is secured using snapper clamp or cable tie wrap. The end is closed with snapper clamp or cable tie wrap.

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# Product Name Product Code Price
2 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
3 Clamp 25 mm TC 56410665 161.46 USD Add to cart
4 Peristaltic Tubing, size 18, 3.1 m, i.d. 9.5 mm, bioprene 56410619 171.81 USD Add to cart
5 Tubing Valve for Tubing o.d. 9.5 mm–13 mm 56410589 77.62 USD Add to cart
Pressure Gauge 0-2 barg (0-30 psig) 50 mm (1.5") TC, back mount, autoclavable 56410603 998.00 USD Add to cart
Pressure Gauge 0-4 barg (0-60 psig) 50 mm (1.5") TC, back mount, autoclavable 56410604 998.00 USD Add to cart
Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, bioprene 56410622 590.00 USD Add to cart
Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, silicone 56410623 628.00 USD Add to cart
Clamp 25 mm TC 56410666 115.92 USD Add to cart
Clamp 64 mm (2") TC 56410670 87.98 USD Add to cart
Manual Permeate Control Kit, includes vacuum/pressure gauge, backpressure valve, supports, adapters, clamps, and gaskets 56410773 2,222.00 USD Add to cart
25 mm (0.5") TC Blank-Off Cap, polysulfone 56410989 61.06 USD Add to cart
Gasket 64 mm (2") TC i.d. 50 mm, silicone 56410998 108.68 USD Add to cart

When running FlexStand Holder with peristaltic pump, the maximum pressure that can be achieved is 2 bar g (30 psi g).

The use of a permeate flow control is strongly recommended during upstream clarification of target material. 

A permeate control valve or a permeate flow control kit together with a pump for the flow rate is required for permeate flow control. Permeate flow control rate is dependent on the process flux (LMH) and surface area. 

Detailed information about the Permeate flow control kit (56-4107-73), Permeate control valve (56-4105-89) and peristaltic pump, flow rates 5.4 ml/min - 2 liter/min (56-4106-53), is available below. 

For countries using 220 V power, power cords have to be ordered separately for the feed and permeate flow control pumps.

Feed pump (11-0005-66 or 11-0005-67), Feed reservoir (56-4107-67 or 56-4107-70), Outlet pressure gauge for FS-03LVS (56-4106-04), Outlet pressure gauge for FS-03LVS-30 ( 56-4106-03) and Tubing (56-4106-19) have to be ordered separately. Detailed information about these products is available below.

After use, store the cartridge with liquid inside using blank off cap, gasket and clamp on the feed and retentate ports.

Seal the permeate port with a short piece of tubing i.d. 9.5 mm, which is secured using snapper clamp, pinch valve or cable tie wrap. The end is closed with pinch clamp, snapper clamp or cable tie wrap.

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# Product Name Product Code Price
2 Clamp 25 mm TC 56410665 161.46 USD Add to cart
3 QuixStand Accessory Kit 56410750 454.00 USD Add to cart
4 Peristaltic Tubing, size 18, 3.1 m, i.d. 9.5 mm, bioprene 56410619 171.81 USD Add to cart
5 Tubing Valve for Tubing o.d. 9.5 mm–13 mm 56410589 77.62 USD Add to cart
6 QuixStand Accessory Kit 56410750 454.00 USD Add to cart
6 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
Pressure Gauge 0-2 barg (0-30 psig) 50 mm (1.5") TC, back mount, autoclavable 56410603 998.00 USD Add to cart
Pressure Gauge 0-4 barg (0-60 psig) 50 mm (1.5") TC, back mount, autoclavable 56410604 998.00 USD Add to cart
Clamp 25 mm TC 56410666 115.92 USD Add to cart
Clamp 64 mm (2") TC 56410670 87.98 USD Add to cart
Manual Permeate Control Kit, includes vacuum/pressure gauge, backpressure valve, supports, adapters, clamps, and gaskets 56410773 2,222.00 USD Add to cart
25 mm (0.5") TC 9.5 mm (0.375") Barb 56410980 132.48 USD Add to cart
25 mm (0.5") TC Blank-Off Cap, polysulfone 56410989 61.06 USD Add to cart
Gasket 64 mm (2") TC i.d. 50 mm, silicone 56410998 108.68 USD Add to cart

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# Product Name Product Code Price
3 Drain Valve Kit for FS-01S, includes custom tee, sanitary plug valve, gasket, and clamp 56410772 1,910.00 USD Add to cart

When running FlexStand Holder with peristaltic pump, the maximum pressure that can be achieved is 2 bar g (30 psi g).

The use of a permeate flow control is strongly recommended during upstream clarification of target material. For permeate flow control you need a valve and pump for the flow rate required. Permeate flow control rate is dependent on the process flux (LMH) and surface area.

Suitable peristaltic pump (56-4106-53) for permeate flow control, flow rates 5.4 ml/min - 2 liter/min

 

Feed pump, feed reservoir, outlet pressure gauge and tubing have to be ordered separately.

Reservoirs (56-4107-67)

Feed pumps (11-0005-66)

Outlet pressure gauge for FS-03LVS (56-4106-04)

Outlet pressure gauge for FS-03LVS-30 (56-4106-03)

Tubing (56-4106-19)

 

FlexStand low volume holder can be converted to FlexStand standard holder, suitable for Housing 5-9, by using a conversion kit.

For countries using 220 V power, power cords have to be ordered separately for the feed and permeate flow control pumps.

After use, store the cartridge with liquid inside using blank off cap, gasket and clamp on the feed and retentate ports.

Seal the permeate port with a short piece of tubing i.d. 13 mm, which is secured using snapper clamp or cable tie wrap. The end is closed with snapper clamp, pinch valve or cable tie wrap.

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# Product Name Product Code Price
2 Gasket 50 mm (1.5") TC i.d. 38 mm, silicone 56410996 92.12 USD Add to cart
3 Clamp 50 mm (1.5") TC (Toggle Clamp) 56410669 78.66 USD Add to cart
4 Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, bioprene 56410622 590.00 USD Add to cart
4 Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, silicone 56410623 628.00 USD Add to cart
7 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
8 Clamp 25 mm TC 56410665 161.46 USD Add to cart
Pressure Gauge 0-2 barg (0-30 psig) 50 mm (1.5") TC, back mount, autoclavable 56410603 998.00 USD Add to cart
Pressure Gauge 0-4 barg (0-60 psig) 50 mm (1.5") TC, back mount, autoclavable 56410604 998.00 USD Add to cart
Peristaltic Tubing, size 18, 3.1 m, i.d. 9.5 mm, bioprene 56410619 171.81 USD Add to cart
Clamp 50 mm TC 56410667 117.99 USD Add to cart
Clamp 50 mm TC 56410668 78.66 USD Add to cart
Clamp 64 mm (2") TC 56410670 87.98 USD Add to cart
Manual Permeate Control Kit, includes vacuum/pressure gauge, backpressure valve, supports, adapters, clamps, and gaskets 56410773 2,222.00 USD Add to cart
25 mm (0.5") TC Blank-Off Cap, polysulfone 56410989 61.06 USD Add to cart
50 mm (1.5") TC Blank-Off Cap, polysulfone 56410990 89.01 USD Add to cart
Gasket 64 mm (2") TC i.d. 50 mm, silicone 56410998 108.68 USD Add to cart

When running FlexStand Holder with peristaltic pump, the maximum pressure that can be achieved is 2 bar g (30 psi g).

The use of a permeate flow control is strongly recommended during upstream clarification of target material. 

A permeate flow control kit together with a pump for the flow rate is required for permeate flow control. Permeate flow control rate is dependent on the process flux (LMH) and surface area. Detailed information about Permeate control valve (56-4105-88) and Permeate flow control kit (56-4107-73), is available below.

Suitable peristaltic pump for permeate flow control:
Flow rates 0.01 ml/min - 8.3 ml/min (18-1110-91). Permeate tubing i.d. 3.1 mm is suitable for the recommended pump.
Flow rates 5.4 ml/min - 2 liter/min (56-4106-53)

Feed pump (56-4106-53), Feed reservoir (56-4107-67), Outlet pressure gauge for FS-03LVS (56-4106-04), Outlet pressure gauge for FS-03LVS-30 ( 56-4106-03) and Tubing (56-4106-17) have to be ordered separately.

For countries using 220 V power, power cords have to be ordered separately for the pump, model no PRP-09WM, code no 56-4106-53.

After use, store the cartridge with liquid inside using blank off cap, gasket and clamp on the feed and retentate ports. Seal the permeate port with a short piece of tubing i.d. 3.1 mm, which is secured using cable tie wrap. The end is closed with snapper clamp or cable tie wrap.

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# Product Name Product Code Price
2 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
3 Clamp 25 mm TC 56410665 161.46 USD Add to cart
4 Peristaltic Tubing, size 16, 3.1 m, i.d. 3.1 mm, silicone 56410617 134.55 USD Add to cart
5 Tubing Valve for Tubing o.d. 6.4 mm 56410588 42.43 USD Add to cart
Peristaltic Pump P-1 18111091 3,793.00 USD Add to cart
Peristaltic Tubing, size 17, 3.1 m, i.d. 6.4 mm, bioprene 56410618 153.18 USD Add to cart
Clamp 25 mm TC 56410666 115.92 USD Add to cart
Clamp 64 mm (2") TC 56410670 87.98 USD Add to cart
Manual Permeate Control Kit, includes vacuum/pressure gauge, backpressure valve, supports, adapters, clamps, and gaskets 56410773 2,222.00 USD Add to cart
25 mm (0.5") TC Blank-Off Cap, polysulfone 56410989 61.06 USD Add to cart
Gasket 64 mm (2") TC i.d. 50 mm, silicone 56410998 108.68 USD Add to cart

Spare parts

The picture below shows FlexStand Low Void Volume Holder FS-03LVS. For differences between the FlexStand Low Void Volume Holders FS-03LVS and FS-03LVS-30 see the table below.

Model no Code no Description
FS-03LVS 56-4107-55 FlexStand low void volume cartridge holder with pressure gauge 0-4 bar g (0-60 psi g)
Model no PG-TCP60, Code no 56-4106-04
FS-03LVS-30 56-4109-44 FlexStand low void volume cartridge holder with pressure gauge 0-2 bar g (0-30 psi g)
Model no PG-TCP30, Code no 56-4106-03


The picture shows a FlexStand Low Void Volume Holder (Connections: 25 mm TC) with a cartridge (Feed and retentate connections: 50 mm TC) installed. Please note that the cartridge is not supplied with the holder. 

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# Product Name Product Code Price
1 Pressure Gauge 0-2 barg (0-30 psig) 50 mm (1.5") TC, back mount, autoclavable 56410603 998.00 USD Add to cart
1 Pressure Gauge 0-4 barg (0-60 psig) 50 mm (1.5") TC, back mount, autoclavable 56410604 998.00 USD Add to cart
2 Clamp 50 mm (1.5") TC (Toggle Clamp) 56410669 78.66 USD Add to cart
2 Gasket 50 mm (1.5") TC i.d. 38 mm, silicone 56410996 92.12 USD Add to cart
3 Clamp 25 mm TC 56410665 161.46 USD Add to cart
3 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
4 25 mm (0.5") TC Blank-Off Cap, polysulfone 56410989 61.06 USD Add to cart
4 50 mm (1.5") TC Blank-Off Cap, polysulfone 56410990 89.01 USD Add to cart
7 Peristaltic Tubing, size 16, 3.1 m, i.d. 3.1 mm, silicone 56410617 134.55 USD Add to cart
7 Peristaltic Tubing, size 17, 3.1 m, i.d. 6.4 mm, bioprene 56410618 153.18 USD Add to cart
7 Peristaltic Tubing, size 18, 3.1 m, i.d. 9.5 mm, bioprene 56410619 171.81 USD Add to cart
7 Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, bioprene 56410622 590.00 USD Add to cart
8 Elbow 25 mm (1/2") TC 56410703 107.64 USD Add to cart
8 25 mm (0.5") TC 9.5 mm (0.375") Barb 56410980 132.48 USD Add to cart
8 25 mm TC 13 mm (0.5") Barb 56410981 202.00 USD Add to cart
Clamp 50 mm TC 56410667 117.99 USD Add to cart
Clamp 50 mm TC 56410668 78.66 USD Add to cart

Seals and tubing are considered as consumables and should be replaced annually or when needed. Inspect the seals and tubing for wear regularly. Please keep a set of each in your stock to prevent long downtime. For code numbers refer to the table below.

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# Product Name Product Code Price
1 Gasket 25 mm (0.5") TC i.d. 13 mm, silicone 56410994 78.66 USD Add to cart
2 Gasket 25 mm (0.75") TC i.d. 19 mm, silicone 56410995 78.66 USD Add to cart
3 Gasket 50 mm (1.5") TC i.d. 38 mm, silicone 56410996 92.12 USD Add to cart
4 Peristaltic Tubing, size 16, 3.1 m, i.d. 3.1 mm, silicone 56410617 134.55 USD Add to cart
5 Peristaltic Tubing, size 17, 3.1 m, i.d. 6.4 mm, bioprene 56410618 153.18 USD Add to cart
6 Peristaltic Tubing, size 18, 3.1 m, i.d. 9.5 mm, bioprene 56410619 171.81 USD Add to cart
7 Peristaltic Tubing, size 82, 7.6 m, i.d. 13 mm, bioprene 56410622 590.00 USD Add to cart

Troubleshooting

Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.

Permeate flow is not adequate (microfiltration membrane)

Feed pressure is too low

Feed pressure is too high

General advice for troubleshooting manual filtration systems

For Manual Filtration Systems (QuixStand, FlexStand, GrandStand, etc.), please check the following during troubleshooting:


Power supply is suitable for running system. Please ensure that mains power supply FlowTech LabTop Rotary Lobe Pumps (used in FlexStand, KvickLab and KvickFlow Systems) is unprotected power.

Do not allow any large hard particles to enter the fluid path, as this will seize (gall) the internal machined surfaces of the pump head. A 50-mesh screen (“y-strainer with removable screen basket) is recommended.

Flow to inlet of rotary lobe pumps is not blocked. Also ensure that there is a gravity head pressure or other means to deliver process fluid to the pump head, as these rotary lobe pumps do not have suction lift capability.

Tank outlet valve is open and the recirculation loop is not blocked. Also check that the flow diameter is not restricted or becomes more narrow than that of the flow diameter at the inlet side of the pump. Finally make sure that the flow rates required can be delivered by the diameter of tubing or pipe used in the systems

Samples volume and system working volume have been used in calculations of concentration factor and diafiltration beforehand

Process settings of Membrane Capacity, Flux, Shear (or volumetric cross flow rate) have been properly applied for both sample characteristics and membrane pore size or NMWC designation.

For peristaltic pump-driven systems make sure the correct tubing sizes and polymers match the manufacturer’s required specifications for that pump head.

Make sure that peristaltic tubing is replaced regularly to avoid excessive wear or tubing failure.

Dripping liquid

Unexpected retention of target molecule

Permeate flow is not adequate (ultrafiltration membrane)

Unexpected passage of target protein to the permeate side

Table of permeate connections requiring tubing only:

Cartridge Model Number Permeate connections o.d.
(2 per cartridge)
Recommended tubing size Order No
(Catalog Model Number)
xFP-x-x-3MA 0.25 inch (6.4mm) tubing nipple Size 17, i.d. 0.25 inch (6.4mm) 56-4106-18 (PTPM06-10)
xFP-x-x-3X2MA 0.25 inch (6.4mm) tubing nipple Size 17, i.d. 0.25 inch (6.4mm) 56-4106-18 (PTPM06-10)
xFP-x-x-4A 0.38 inch (9.5mm) tubing nipple Size 18, i.d. 0.38 inch (9.5mm) 56-4106-19 (PTPM09-10)
xFP-x-x-4MA 0.38 inch (9.5mm) tubing nipple Size 18, i.d. 0.38 inch (9.5mm) 56-4106-19 (PTPM09-10)
xFP-x-x-4X2MA 0.38 inch (9.5mm) tubing nipple Size 18, i.d. 0.38 inch (9.5mm) 56-4106-19 (PTPM09-10)
xFP-x-x-5A 0.5 inch (13mm) tubing nipple i.d. 0.5 inch (13mm) 56-4106-22 (PTPM12)
xFP-x-x-6A 0.5 inch (13mm) tubing nipple i.d. 0.5 inch (13mm) 56-4106-22 (PTPM12)
xFP-x-x-8A 0.5 inch (13mm) tubing nipple i.d. 0.5 inch (13mm) 56-4106-22 (PTPM12)
xFP-x-x-9A 0.5 inch (13mm) tubing nipple i.d. 0.5 inch (13mm) 56-4106-22 (PTPM12)

 

Leakage during integrity testing

Permeate pressure is dropping

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