Spare parts
# | Product Name | Product Code | Price | |
---|---|---|---|---|
1 | Clamp with spring | 80614783 | 128.16 USD |
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|
3 | Gasket | 80613719 | 64.42 USD |
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|
# | Product Name | Product Code | Price | |
---|---|---|---|---|
1 | Wax paper sheet | 80613567 | 57.60 USD |
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|
2 | Gel seal | 80642143 | 38.86 USD |
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|
3 | Gel seal | 80642143 | 38.86 USD |
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|
8 | Wonder wedge, molded | 80612788 | 107.37 USD |
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|
Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Heavy background during silver staining
Possible cause | Suggested remedy |
---|---|
Acrylamide or bis acrylamide contains acrylic acid, a breakdown product |
Use reagents specified as electrophoresis grade in purity. |
Water is impure. |
Use only double-distilled water. |
Bromophenol blue doesn't sharpen into a concentrated zone in the stacking gel.
Possible cause | Suggested remedy |
---|---|
The pH of the stacking gel or running buffer is incorrect |
Check buffer stocks and casting recipe. |
Too high sodium or potassium concentration. |
Avoid using solutions with a high sodium or potassium concentration |
Issues with the acrylamide solution |
|
- |
For best results, allow a stacking gel height of 2.5 times the height of the sample in the well |
Tracking dye doesn’t sharpen into a concentrated zone in the stacking gel
Possible cause | Suggested remedy |
---|---|
Poor stacking |
Pour a taller stacking gel. (For best results, allow a stacking-gel height of 2.5 times the height of the sample in the well.) |
Reagent quality |
Dispose of outdated acrylamide solutions and use only the highest grade of acrylamide |
Sample preparation |
When preparing samples, avoid using solutions with high salt concentrations |
Brittle gel
Possible cause | Suggested remedy |
---|---|
Too much bisacrylamide. |
Crosslinker should be at 2.6 %C for standard SDS (Sodium dodecyl sulfate) gels where: |
Swirl patterns in gel
Possible cause | Suggested remedy |
---|---|
Too much catalyst: gel polymerized in < 10 min |
Reduce both APS (Ammonium persulphate) and TEMED by 25%. |
Not enough catalyst: gel polymerized in > 50 min |
Increase both APS (Ammonium persulphate) and TEMED by 50% |
Solutions not mixed |
Mix thoroughly after adding TEMED |
Gels adhere to glass plate when opening sandwich
Possible cause | Suggested remedy |
---|---|
Dirty plates e.g. fingerprints |
Soak plates in a strong laboratory detergent and rinse well in distilled water. Please use gloves. |
Plates are scratched |
Replace glass plates. To some extent the effect of scratches can be counteracted by treating plates with silanizing reagent such as Repel-Silane. |
Plates were stored with, or soaked together with, plates that were pre-treated with Bind-Silane. |
Always segregate Bind-Silanized plates. Bind-Silanized plates can "contaminate" untreated plates if placed in contact, or soaked together. Glass plates can be re-used after scraping off the polyacrylamide gel and thoroughly washing the glass plates with strong sodium hydroxide solution. |
Incomplete gel polymerization.
Possible cause | Suggested remedy |
---|---|
Poor chemicals |
1. Use only recent stock of the highest quality reagents. |
Solutions with extreme pH values (especially acidic) may not polymerize. |
- |
Oxygen is present |
Remove oxygen from the gel environment: Degas the monomer solution 5 to 10 minutes before pouring and then overlay the gel surface with water-saturated n-butanol. |
Gel solution has too low temperature |
Adjust the gel solution temperature to a minimum of 20 °C, especially for low %T (Total acrylamide concentration) gels. |
Too low concentration of TEMED or APS (Ammonium persulphate) |
Increase TEMED or APS concentration, or both |