FAQ
When should I use SDS-PAGE Clean-up kit ?
The SDS-PAGE Clean-up kit is primarily a concentration tool.
What is the difference between the 2-D Clean-Up Kit and the SDS-PAGE Clean-Up Kit?
Four of the reagents (Precipitant, Co-precipitant, Wash buffer and Wash additive) are identical. The SDS-PAGE Clean-Up Kit has three additional reagents (Buffer I, Buffer II and SDS-PAGE sample buffer). The additional reagents are for optimal solubilization in a small volume of SDS-PAGE-compatible sample buffer.
Troubleshooting
Find solutions to product related issues. For unlisted issues please contact local Cytiva service representation.
Select a symptom:
Possible cause | Suggested remedy |
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Pellet is too dry or a large amount of protein is present. |
Use additional buffer I, sonicate or use the PlusOne Sample Grinding Kit to facilitate resuspension. |
Possible cause | Suggested remedy |
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Insufficient washing with wash buffer. |
Add buffer I in increments of 0.5 μl until the solution turns blue. |